| Project/Area Number |
23700361
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 無細胞翻訳系 / 人工細胞 / エマルション / ガラクトシダーゼ / グルクロニダーゼ / 蛍光検出 / 速度論 / PURE system / 細胞サイズ / 区画化 / 細胞内反応 / 微小反応場 |
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| Research Abstract |
The cell contents are encapsulated within a compartment, the volume of which is a fundamental physical parameter that may affect intracompartmental reactions. However, there have been few studies to elucidate whether and how volume changes alone ca n affect the reaction kinetics. Here, we prepared artificial cell-like compartments with different volumes, and encapsulated the in vitro protein synthesis system. The effect of the compartment size on the protein synthesis reaction was investigated. We found a reaction that proceeds faster in smaller compartments and revealed the mechanism of the acceleration. This result provided an example of how small compartment can be beneficial for the intracompartmental reactions.
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