Understanding CaMKII phosphorylation by in vitro reconstitution experiments and model simulation
| Project/Area Number |
23700371
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neuroscience in general
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| Research Institution | Kyoto University (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | CaMKII / 再構成実験 / NMDA受容体 / シナプス可塑性 / システム生物学 / シミュレーション |
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| Research Abstract |
Ca2+/Calmodulin-dependent protein kinase II (CaMKII) has been shown to play a major role in establishing memories through complex molecular interactions including phosphorylation of multiple synaptic targets. Here we reconstituted Ca2+/Calmodulin-dependent CaMKII autophosphorylation in the presence of protein phosphatase 1 in vitro, and found that CaMKII phosphorylation shows a switch-like response with history dependence (hysteresis) only in the presence of an N-methyl-D-aspartate (NMDA) receptor-derived peptide. This hysteresis may be crucial for induction and maintenance of long-term synaptic plasticity at synapses.
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Report
(4 results)
Research Products
(20 results)
