Axonal regeneration via regulation of point contacts

• Project/Area Number: 23700376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: University of Toyama
• Principal Investigator: KUBOYAMA TOMOHARU 富山大学, 和漢医薬学総合研究所, 助教 (10415151)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞接着 / 脊髄損傷 / 軸索再生 / プロテオグリカン / プロテインキナーゼA / パキシリン / p21-activated kinase / コンドロイチン硫酸プロテオグリカン / dystrophic endball / 細胞接着班

Research Abstract

I previously clarified that enhancing of point contact dynamics promoted axonal regeneration in cultured system that mimicked failure of axonal regeneration in the injured central nervous system. In this study, I showed that protein kinase A, p21-activated kinase and paxillin were involved in the above regeneration mechanisms. This study proposes novel targets for axonal regeneration in spinal cord injury, etc.

Research Products

• [Journal Article] New reliable scoring system, Toyama Mouse Score, to evaluate locomotor function following spinal cord injury in mice (2014)
  • Author(s): Shigyo M, Tanabe N, Kuboyama T, Choi S-H, Tohda C
  • Journal Title: BMC Res Notes Volume: 7(1) Pages: 332-332
  • Peer Reviewed
• [Journal Article] Effects of Ashwagandha (roots of Withania somnifera) on neurodegenerative diseases (2014)
  • Author(s): Kuboyama T, Tohda C, Komatsu K
  • Journal Title: Biol Pharm Bull Volume: 37(6) Pages: 892-897
  • NAID: 130004147346
  • Peer Reviewed
• [Journal Article] New perspective in pharmacology brought by studying traditional medicine (2014)
  • Author(s): Tohda C, Kuboyama T
  • Journal Title: Nihon Yakurigaku Zasshi Volume: 143(2) Pages: 73-77
  • NAID: 130003391427
  • Peer Reviewed
• [Journal Article] Effects of Ashwagandha (roots of Withania somnifera) on neurodegenerative diseases. (2014)
  • Author(s): Kuboyama T, Tohda C, Komatsu K.
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 37
  • NAID: 130004147346
  • Peer Reviewed
• [Journal Article] Paxillin phosphorylation counteracts proteoglycan-mediated inhibition of axon regeneration (2013)
  • Author(s): Tomoharu Kuboyama, Xueting Luo, Kevin Park, Murray G. Blackmore, Takuro Tojima, Chihiro Tohda, John L. Bixby, Vance P. Lemmon, Hiroyuki Kamiguchi
  • Journal Title: Experimental Neurology Volume: 248 Pages: 157-169
  • DOI: 10.1016/j.expneurol.2013.06.011
  • Peer Reviewed
• [Journal Article] A novel compound, denosomin, ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin. (2013)
  • Author(s): Teshigawara K, Kuboyama T, Shigyo M, Nagata A, Sugimoto K, Matsuya Y, Tohda C.
  • Journal Title: British Journal of Pharmacology Volume: 168 Issue: 4 Pages: 903-919
  • DOI: 10.1111/j.1476-5381.2012.02211.x
  • Peer Reviewed
• [Journal Article] Screening on functions from some Chinese herbs for spinal cord injury (2012)
  • Author(s): Wang XH, Zhu H, Konno K, Tohda C, Kazuma K, Kuboyama T, Miyanaga S
  • Journal Title: Chinese Journal of Experimental Traditional Medical Formulae Volume: 18(12) Pages: 246-248
  • Peer Reviewed
• [Journal Article] Diosgenin is an exogenous activator of 1,25D3-MARRS/Pdia3/ERp57 and improves Alzheimer’s disease pathologies in 5XFAD mice. (2012)
  • Author(s): Tohda C, Urano T, Umezaki M, Nemere I, Kuboyama T.
  • Journal Title: Sci. Rep., Volume: 2 Issue: 1 Pages: 535-535
  • DOI: 10.1038/srep00535
  • Peer Reviewed
• [Journal Article] Screening on functions from some Chinese herbs for spinal cord injury. (2012)
  • Author(s): Wang XH, Zhu H, Konno K, Tohda C, Kazuma K, Kuboyama T, Miyanaga S
  • Journal Title: Chinese Journal of Experimental Traditional Medical Formulae Volume: 18 Pages: 246-248
  • Peer Reviewed
• [Journal Article] Current and future therapeutic strategies for functional repair of spinal cord injury (2011)
  • Author(s): Tohda C
  • Journal Title: Pharmacology & Therapeutics Volume: 132 Issue: 1 Pages: 57-71
  • DOI: 10.1016/j.pharmthera.2011.05.006
  • Peer Reviewed
• [Journal Article] Kamikihi-to (KKT) rescues axonal and synaptic degeneration associated with memory impairment in a mouse model of Alzheimer's disease, 5XFAD (2011)
  • Author(s): Tohda C, Nakada R, Urano T, Okonogi A, Kuboyama T
  • Journal Title: Int. J. Neurosci Volume: 121(12) Issue: 12 Pages: 641-648
  • DOI: 10.3109/00207454.2011.602809
  • Peer Reviewed
• [Journal Article] Current and future therapeutic strategies for functional repair of spinal cord injury. (2011)
  • Author(s): Tohda C, Kuboyama T.
  • Journal Title: Pharmacology & Therapeutics Volume: 132 Pages: 57-71
  • Peer Reviewed
• [Presentation] DR-induced cognitive enhancement in Alzheimer's disease model mice, 5XFAD, and underlying mechanisms (2014)
  • Author(s): Yang Z., Kuboyama T., Tohda C.
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] Extracellular vimentin promotes axonal growth via IGF1R (2014)
  • Author(s): Shigyo M., Kuboyama T., Tohda C.
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] Improvement of motor dysfunction in acute and chronic phases in spinal cord injury mice by a crude drug-derived compound (2014)
  • Author(s): Tanabe N., Kuboyama T., Kazuma K., Konnno K., Tohda C.
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] Clathrin-mediated endocytosis is a key target for amyloid β-induced axonal degeneration and memory impairment (2014)
  • Author(s): Kuboyama T., Lee Y., Nishiko H., Tohda C.
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] Extracellular vimentin facilitates axonal growth by interacting with IGF1R (2014)
  • Author(s): Shigyo M., Kuboyama T., Chihiro T.
  • Organizer: 第57回日本神経化学大会
  • Place of Presentation: 奈良
• [Presentation] DR improves memory impairment in Alzheimer's disease model mice, 5XFAD, and attenuates amyloid β-induced axonal atrophy (2014)
  • Author(s): Yang Z., Kuboyama T., Tohda C.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] New reliable scoring system, Toyama Mouse Score, to evaluate locomotor function following spinal cord injury in mice (2014)
  • Author(s): Shigyo M., Tanabe N., Kuboyama T., Coi S.-H., Chihiro T.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] The recovery of motor function by a crude drug and its active constituent in spinal cord injured mice (2014)
  • Author(s): Tanabe N., Kuboyama T., Kazuma K., Konnno K., Tohda C.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Inhibition of clathrin-mediated endocytosis prevents amyloid β-induced growth cone collapse, axonal atrophy and memory impairment (2014)
  • Author(s): Kuboyama T., Lee Y., Nishiko H., Tohda C.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 日本および韓国で用いられている加味帰脾湯の記憶増強作用の比較 (2014)
  • Author(s): 渡り英俊、執行美智子、田辺紀生、東田道久、 Ki-Ho Cho、島田豊、柴原直利、久保山友晴、東田千尋
  • Organizer: 第31回和漢医薬学学会学術大会
  • Place of Presentation: 千葉
• [Presentation] 脊髄損傷後の機能回復に低分子量タンパク質Racが関与する (2013)
  • Author(s): 甲田将章、上山健彦、執行美智子、久保山友晴、東田千尋、齋藤尚亮、甲村英二
  • Organizer: 日本脳神経外科学会第72回学術総会
  • Place of Presentation: 横浜
• [Presentation] 脊髄損傷の運動機能改善効果を持つ漢方生薬の研究 (2013)
  • Author(s): 田辺紀生、久保山友晴、数馬恒平、紺野勝弘、東田千尋
  • Organizer: 第30回和漢医薬学会学術大会
  • Place of Presentation: 金沢
• [Presentation] Ashwagandha成分 Sominoneの誘導体Denosominのアストロサイトを介した脊髄損傷改善作用 (2013)
  • Author(s): 執行美智子、久保山友晴、東田千尋
  • Organizer: 第30回和漢医薬学会学術大会
  • Place of Presentation: 金沢
• [Presentation] Endocytosis-mediated axonal growth cone degeneration induced by amyloid β (2013)
  • Author(s): Kuboyama T, Nishiko H, Tohda C
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Diosgenin is an exogenous activator of 1,25D3-MARRS (Pdia3/ERp57) and improves memory dysfunction and axonal degeneration in Alzheimer's disease model 5XFAD mice (2013)
  • Author(s): Tohda C, Urano T, Umezaki M, Kuboyama T
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] A novel compound, Denosomin, promotes axonal growth via astrocytes secreting vimentin after spinal cord injury (2013)
  • Author(s): Shigyo M, Teshigawara K, Kuboyama T, Nagata A, Sugimoto K, Matsuya Y, Tohda C
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Investigation of crude drugs effective for spinal cord injured mice (2013)
  • Author(s): Tanabe N, Kuboyama T, Miyanaga S, Kazuma K, Konno K, Tohda C
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] 新規化合物 denosominはアストロサイトからの vimentin放出を促進して脊髄損傷マウスの運動機能を回復させる (2013)
  • Author(s): 久保山友晴、執行美智子、東田千尋
  • Organizer: 平成25年度神経糖鎖生物学夏の斑会議
  • Place of Presentation: 守山、滋賀
• [Presentation] Diosgenin is an exogenous activator of 1,25D3-MARRS (Pdia3/ERp57) and improves memory dysfunction and axonal degeneration in Alzheimer’s disease model 5XFAD mice (2013)
  • Author(s): Tohda C, Urano T, Umezaki M, Kuboyama T
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Investigation of crude drugs effective for spinal cord injured mice. (2013)
  • Author(s): Tanabe N, Kuboyama T, Miyanaga S, Kazuma K, Konno K, Tohda C
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Ashwagandha成分Sominoneの誘導体Denosominのアストロサイトを介した脊髄損傷改善作用 (2013)
  • Author(s): 執行美智子、久保山友晴、東田千尋
  • Organizer: 第30回和漢医薬学会学術大会
  • Place of Presentation: 金沢
• [Presentation] 脊髄損傷後の機能回復に低分子量タンパク質Racが関与する (2013)
  • Author(s): 甲田将章、上山健彦、執行美智子、久保山友晴、東田千尋、齋藤尚亮、甲村英二
  • Organizer: 日本脳神経外科学会 第72回学術総会
  • Place of Presentation: 横浜
• [Presentation] A novel compound, denosomin ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin (2012)
  • Author(s): Kuboyama T
  • Organizer: 1st Joint Symposium Between Natural Products Research Institute, College of Pharmacy, Seoul National University and Institute of Natural Medicine, University of Toyama
  • Place of Presentation: Toyama
  • Year and Date: 2012-12-15
• [Presentation] Diosgenin is an exogenous activator of 1,25D3-MARRS (Pdia3/ERp57) and improves memory dysfunction and axonal degeneration in Alzheimer's disease model 5XFAD mice (2012)
  • Author(s): Tohda C, Urano T, Umezaki M, Nemere I, Kuboyama T
  • Organizer: Neuroscience2012
  • Place of Presentation: New Orleans, LA, U.S.A.
• [Presentation] Denosomin improves SCI via axonal growth by increasing astrocyte-secreted vimentin (2012)
  • Author(s): Shigyo M, Kuboyama T, Teshigawara K, Sugimoto K, Matsuya Y, Tohda C
  • Organizer: Neuroscience2012
  • Place of Presentation: New Orleans, LA, U.S.A.
• [Presentation] A novel compound, Denosomin induces vimentin-secretion from astrocytes, resulting in promotion of axon elongation (2012)
  • Author(s): Kuboyama T, Teshigawara K, Shigyo M, Sugimoto K, Matsuya Y, Tohda C
  • Organizer: Neuroscience2012
  • Place of Presentation: New Orleans, LA, U.S.A.
• [Presentation] Novel mechanism of functional recovery from spinal cord injury (2012)
  • Author(s): Tohda C, Kuboyama T, Teshigawara K, Shigyo M
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto
• [Presentation] 阻害性プロテオグリカンによる軸索再生阻害機構の解明 (2012)
  • Author(s): 久保山友晴、Vance Lemmon、Jerry Silver、東田千尋、上口裕之
  • Organizer: 2012年度包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台
• [Presentation] コンドロイチン硫酸プロテオグリカン濃度勾配による軸索再生阻害のシグナル伝達機構 (2012)
  • Author(s): 久保山友晴
  • Organizer: 第2回「神経糖鎖生物学」領域会議
  • Place of Presentation: 淡路、兵庫
• [Presentation] Diosgenin is an exogenous activator of 1,25D3-MARRS (Pdia3/ERp57) and improves memory dysfunction and axonal degeneration in Alzheimer’s disease model 5XFAD mice (2012)
  • Author(s): Tohda C, Urano T, Umezaki M, Nemere I, Kuboyama T
  • Organizer: Neuroscience2012
  • Place of Presentation: New Orleans, LA, U.S.A.
• [Presentation] Novel mechanism of functional recovery from spinal cord injury (2012)
  • Author(s): Tohda C, Kuboyama T, Teshigawara K, Shigyo M
  • Organizer: 4th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都市
  • Invited
• [Presentation] A novel compound, denosomin ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin (2012)
  • Author(s): Kuboyama T
  • Organizer: 1st Joint Symposium Between Natural Products Research Institute, College of Pharmacy, Seoul National University and Institute of Natural Medicine, University of Toyama
  • Place of Presentation: 富山市
  • Invited
• [Presentation] コンドロイチン硫酸プロテオグリカン濃度勾配による軸索再生阻害のシグナル伝達機構 (2012)
  • Author(s): 久保山友晴
  • Organizer: 第2回「神経糖鎖生物学」領域会議
  • Place of Presentation: 淡路市
• [Presentation] 阻害性プロテオグリカンによる軸索再生阻害機構の解明 (2012)
  • Author(s): 久保山友晴、Vance Lemmon、Jerry Silver、東田千尋、上口裕之
  • Organizer: 2012年度 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台市
• [Presentation] コンドロイチン硫酸プロテオグリカンによる軸索再生阻害のシグナル伝達機構 (2011)
  • Author(s): 久保山友晴
  • Organizer: 軸索再生連絡会議
  • Place of Presentation: 和光、埼玉
• [Presentation] Kamikihi-to improves memory impairment and axon degeneration in a mouse model of Alzheimer's disease 5XFAD (2011)
  • Author(s): Nakada R, Kuboyama T, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀、石川
• [Presentation] Denosomin enhances axonal regrowth associated with motor function recovery in spinal cord injury (2011)
  • Author(s): Shigyo M, Nagata A, Teshigawara K, Kuboyama T, Matsuya Y, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀、石川
• [Presentation] 1-deoxy-nor-sominone (Denosomin) improves hindlimb dysfunction in spinal cord injury and promotes neurite outgrowth through astroglia-mediated signaling (2011)
  • Author(s): Teshigawara K, Kuboyama T, Matsuya Y, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀、石川
• [Presentation] Signal transduction underlying chondroitin sulfate proteoglycan-mediated inhibition of axon regeneration (2011)
  • Author(s): Kuboyama T, Silver J, Tohda C, Kamiguchi H
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀、石川
• [Presentation] Searching for natural medicines that improve spinal cord injury (2011)
  • Author(s): Wang X, Kuboyama T, Miyanaga S, Kazuma K, Konno K, Satake M, Tohda C
  • Organizer: 第28回和漢医薬学会学術大会
  • Place of Presentation: 富山
• [Presentation] Signal transduction underlying chondroitin sulfate proteoglycan-mediated inhibition of axon regeneration (2011)
  • Author(s): Kuboyama T, Silver J, Tohda C, Kamiguchi H
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀市
• [Presentation] 1-deoxy-nor-sominone (Denosomin) improves hindlimb dysfunction in spinal cord injury and promotes neurite outgrowth through astroglia-mediated signalling (2011)
  • Author(s): Teshigawara K, Kuboyama T, Matsuya Y, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀市
• [Presentation] Denosomin enhances axonal regrowth associated with motor function recovery in spinal cord injury (2011)
  • Author(s): Shigyo M, Nagata A, Teshigawara K, Kuboyama T, Matsuya Y, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀市
• [Presentation] Kamikihi-to improves memory impairment and axon degeneration in a mouse model of Alzheimer’s disease, 5XFAD (2011)
  • Author(s): Nakada R, Kuboyama T, Tohda C
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 加賀市
• [Remarks] 神経機能学分野研究業績
  • URL: http://www.inm.u-toyama.ac.jp/arcem/gyouseki.html