Analysis of the regulation mechanism of the progenitor cell division pattern along developmental stages

• Project/Area Number: 23700378
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Nagoya University
• Principal Investigator: OKAMOTO Mayumi 名古屋大学, 医学系研究科, COE 特任助教 (30551965)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経幹細胞 / 非対称分裂 / interkinetic nuclear migration / basal突起 / マイクロアレイ / 神経発生

Research Abstract

We found that knockdown of the cell-surface molecule TAG-1, which is enriched basally in early mouse cerebral walls, causes retraction of progenitors’ basal processes. Basally disconnected stem-like progenitors failed to undergo basalward INM and overcrowded in the periventricular space. Surprisingly, the overcrowded progenitors left the apical surface and migrated into basal neuronal territories. Although the heterotopic progenitors unexpectedly remained stem-like, sequentially producing neurons until late embryonic period, histogenesis was severely disrupted. Thus, progenitors’ morphology-dependent INM is essential to prevent nuclear/somal overcrowding, thereby ensuring normal brain histogenesis.

Research Products

• [Journal Article] Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken (2012)
  • Author(s): Kawaue, T., Okamoto, M., Matsuyo, A., Inoue, J., Ueda, Y., Tomonari, S., Noji, S., Ohuchi, H
  • Journal Title: Biology Open Volume: 1 Issue: 11 Pages: 1083-1093
  • DOI: 10.1242/bio.20121396
  • Peer Reviewed
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control (2013)
  • Author(s): Okamoto, M
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松・香川国際会議場(高松市)
  • Year and Date: 2013-03-29
• [Presentation] Interkinetic nuclear migration through TAG-1-assisted progenitor elongation prevents neuroepithelial overcrowding and ensures neocortical histogenesis (2013)
  • Author(s): Okamoto, M
  • Organizer: 第6回神経発生討論会
  • Place of Presentation: 理化学研究所(和光市)
  • Year and Date: 2013-03-14
• [Presentation] Interkinetic nuclear migration prevents neuroepithelial overcrowding and ensures neocortical histogenesis (2013)
  • Author(s): 岡本麻友美
  • Organizer: 新学術領域「動く細胞と場のクロストークによる秩序の生成」第2回若手の会
  • Place of Presentation: ホテル ラフォーレ修善寺(伊豆)
  • Year and Date: 2013-03-07
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control (2013)
  • Author(s): 岡本麻友美
  • Organizer: 第5回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ(大府市)
  • Year and Date: 2013-02-01
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control' (2013)
  • Author(s): Okamoto, M
  • Organizer: The 23rd CDB Meeting Building multicellular systems from cellular cross-talk
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(神戸市)
  • Year and Date: 2013-01-22
• [Presentation] Interkinetic nuclear migration through TAG-1-assisted progenitor elongation prevents neuroepithelial overcrowding and ensures neocortical histogenesis (2013)
  • Author(s): Mayumi Okamoto
  • Organizer: 第６回神経発生討論会
  • Place of Presentation: 理化学研究所 和光キャンパス（埼玉県和光市）
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis (2012)
  • Author(s): Okamoto, M.
  • Organizer: GCOE第4回国際シンポジウム「神経-腫瘍の医学生物学シンポ図3 TAG-1ノックダウンによりbasal突起を失った神経幹細胞の細胞産生能図4 TAG-1ノックダウンによる大脳組織形成の異常 ジウム」
  • Place of Presentation: ウェスティンナゴヤキャッスルホテル(名古屋市)
  • Year and Date: 2012-11-16
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis (2012)
  • Author(s): Okamoto, M
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans (USA)
  • Year and Date: 2012-10-14
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis (2012)
  • Author(s): Okamoto, M
  • Organizer: 第3回名古屋大学・生理研合同シンポジウム
  • Place of Presentation: 第3回名古屋大学・生理研合同シンポジウム
  • Year and Date: 2012-09-29
• [Presentation] TAG1 の消失はapical progenitorの形態と核移動の異常を引き起こし、apical表面での細胞の混みは異所的な細胞産生と皮質の形成異常を導く (2012)
  • Author(s): 岡本麻友美
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2012-09-21
• [Presentation] The basal process of neural stem cells helps interkinetic nuclear migration and neuron/progenitor territorialization (2012)
  • Author(s): Okamoto, M
  • Organizer: ISSCR, International Society for Stem Cell Research 10th Annual Meeting
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2012-06-15
• [Presentation] TAG1 shapes neural progenitor cells and regulates interkinetic nuclear migration and histogenesis in the developing neocortex (2012)
  • Author(s): Okamoto, M
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2012-05-29
• [Presentation] Studying nascent daughter cells' neighborship to understand the mechanism of cell fate choices in the neocortical neurogenesis (2012)
  • Author(s): 岡本麻友美
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(甲府市)
  • Year and Date: 2012-03-26
• [Presentation] 大脳発生過程において神経前駆細胞の形と動きを規定する「場」:初期誕生のニューロンたちの役割 (2012)
  • Author(s): 岡本麻友美
  • Organizer: 新学術領域「動く細胞と場のクロストークによる秩序の生成」の「若手の会」
  • Place of Presentation: 地方職員共済組合有馬保養所瑞宝園(神戸市)
  • Year and Date: 2012-03-17
• [Presentation] The basal process of neural stem cells helps interkinetic nuclear migration and neuron/progenitor territrialization (2012)
  • Author(s): 岡本麻友美
  • Organizer: 第5回神経発生討論会
  • Place of Presentation: 第5回神経発生討論会
  • Year and Date: 2012-03-15
• [Presentation] Early progenitor cells' basal process morphology is regulated by subplate neurons and essential for interkinetic nuclear migration and overall neocortical histogenesis (2012)
  • Author(s): Okamoto, M
  • Organizer: 第1回国際シンポジウム/ The 59th NIBB Confernce "Neocortical Organization"
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(岡崎市)
  • Year and Date: 2012-03-10
• [Presentation] The regulation of neural stem cells' morphology and behavior during cortical development (2012)
  • Author(s): Okamoto, M.
  • Organizer: 第4回NAGOYAグローバルリトリート
  • Place of Presentation: 第4回NAGOYAグローバルリトリート
  • Year and Date: 2012-02-24
• [Presentation] 発生過程の大脳における運命決定相互作用:誕生直後の近隣関係性の探索 (2012)
  • Author(s): 岡本麻友美
  • Organizer: 定量生物学の会 第四回年会
  • Place of Presentation: 名古屋大学野依記念学術交流館(名古屋市)
  • Year and Date: 2012-01-08
• [Presentation] Studying nascent daughter cells' neighborship to better understand the mechanism of cell fate determination in the neocortical and retinal neurogenesis (2011)
  • Author(s): Okamoto, M
  • Organizer: GCOE第3回国際シンポジウム
  • Place of Presentation: ミッドランドホール(名古屋市)
  • Year and Date: 2011-12-08
• [Presentation] 大脳の分化過程における運命決定相互作用:誕生直後の娘細胞の近隣関係性を知るための全細胞タイムラプスイメージング (2011)
  • Author(s): 岡本麻友美
  • Organizer: 第71回日本解剖学会中部支部学術集会
  • Place of Presentation: 名古屋大学医学部講堂(名古屋市)
  • Year and Date: 2011-10-15
• [Presentation] 神経分化過程における細胞の運命決定相互作用:誕生直後の娘細胞の近隣関係性のイメージング (2011)
  • Author(s): 岡本麻友美
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-16
• [Presentation] Studying nascent cells' neighborship to better understand the mechanism of cell-fate determination in neocortical neurogenesis (2011)
  • Author(s): Okamoto, M
  • Organizer: The Cambridge Neural Stem Cell Symposium
  • Place of Presentation: Cambridge (UK)
  • Year and Date: 2011-09-05
• [Presentation] 神経分化過程の細胞運命決定メカニズムをより理解するための、誕生直後の娘細胞の近隣関係性の探索 (2011)
  • Author(s): 岡本麻友美
  • Organizer: 第2回名古屋大学医学系研究科・生理学研究所合同シンポジウム
  • Place of Presentation: 名古屋大学医学部講堂(名古屋市)
  • Year and Date: 2011-08-20
• [Presentation] 大脳前駆細胞において発生時期特異的に発現する遺伝子の機能解析 (2011)
  • Author(s): Okamoto, M
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県宜野湾市)
  • Year and Date: 2011-05-18
• [Presentation] TAG1 shapes neural progenitor cells and regulates interkinetic nuclear migration and histogenesis in the developing neocortex
  • Author(s): Mayumi Okamoto
  • Organizer: 第45回日本発生生物学会･第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場(神戸市)
• [Presentation] The basal process of neural stem cells helps interkinetic nuclear migration and neuron/progenitor territorialization
  • Author(s): Mayumi Okamoto
  • Organizer: ISSCR, International Society for Stem Cell Research 10th Annual Meeting
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] TAG1の消失はapical progenitorの形態と核移動の異常を引き起こし、apical表面での細胞の混みは異所的な細胞産生と皮質の形成異常を導く
  • Author(s): 岡本麻友美
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans, USA
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: 第3回名古屋大学・生理研合同シンポジウム
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター(岡崎市)
• [Presentation] TAG1 deficiency-induced shortening and resultant abnormal nucleokinesis of apical progenitors causes periventricular cellular congestion and heterotopic cytogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: GCOE第4回国際シンポジウム｢神経ｰ腫瘍の医学生物学シンポジウム｣
  • Place of Presentation: ウェスティンナゴヤキャッスルホテル(名古屋市)
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control'
  • Author(s): Mayumi Okamoto
  • Organizer: The 23rd CDB Meeting Building multicellular systems from cellular cross-talk
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(神戸市)
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control'
  • Author(s): 岡本麻友美
  • Organizer: 第5回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ (愛知県大府市)
• [Presentation] Interkinetic nuclear migration prevents neuroepithelial overcrowding and ensures neocortical histogenesis
  • Author(s): 岡本麻友美
  • Organizer: 新学術領域「動く細胞と場のクロストークによる秩序の生成」第2回若手の会
  • Place of Presentation: ホテル ラフォーレ修善寺（伊豆市）
• [Presentation] Interkinetic nuclear migration based on neuroepithelial-cell elongation ensures brain histogenesis through 'crowd control'
  • Author(s): Mayumi Okamoto
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松（高松市）
• [Presentation] 大脳前駆細胞において発生時期特異的に発現する遺伝子の機能解析
  • Author(s): Mayumi Okamoto
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県宜野湾市)
• [Presentation] Studying nascent cells’ neighborship to better understand the mechanism of cell-fate determination in neocortical neurogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: The Cambridge Neural Stem Cell Symposium
  • Place of Presentation: Cambridge (UK)
• [Presentation] 神経分化過程における細胞の運命決定相互作用：誕生直後の娘細胞の近隣関係性のイメージング
  • Author(s): 岡本麻友美
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] 大脳の分化過程における運命決定相互作用：誕生直後の娘細胞の近隣関係性を知るための全細胞タイムラプスイメージング
  • Author(s): 岡本麻友美
  • Organizer: 第71回日本解剖学会中部支部学術集会
  • Place of Presentation: 名古屋大学医学部講堂(名古屋市)
• [Presentation] Studying nascent daughter cells' neighborship to better understand the mechanism of cell fate determination in the neocortical and retinal neurogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: GCOE第3回国際シンポジウム
  • Place of Presentation: ミッドランドホール(名古屋市)
• [Presentation] 発生過程の大脳における運命決定相互作用：誕生直後の近隣関係性の探索
  • Author(s): 岡本麻友美
  • Organizer: 定量生物学の会 第四回年会
  • Place of Presentation: 名古屋大学野依祈念学術交流館(名古屋市)
• [Presentation] The regulation of neural stem cells' morphology and behavior during cortical development
  • Author(s): Mayumi Okamoto
  • Organizer: 第4回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ(大府市)
• [Presentation] Early progenitor cells' basal process morphology is regulated by subplate neurons and essential for interkinetic nuclear migration and overall neocortical histogenesis
  • Author(s): Mayumi Okamoto
  • Organizer: 第1回国際シンポジウム/The 59th NIBB Confernce "Neocortical Organization"
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(岡崎市)
• [Presentation] The basal process of neural stem cells helps interkinetic nuclear migration and neuron/progenitor territrialization
  • Author(s): 岡本麻友美
  • Organizer: 第5回神経発生討論会
  • Place of Presentation: 福井県県民ホール(福井市)
• [Presentation] 大脳発生過程において神経前駆細胞の形と動きを規定する「場」：初期誕生のニューロンたちの役割
  • Author(s): 岡本麻友美
  • Organizer: 新学術領域「動く細胞と場のクロストークによる秩序の生成」の「若手の会」
  • Place of Presentation: 地方職員共済組合有馬保養所瑞宝園(神戸市)
• [Presentation] Studying nascent daughter cells’ neighborship to understand the mechanism of cell fate choices in the neocortical neurogenesis
  • Author(s): 岡本麻友美
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(甲府市)