DJ-1 associates with membrane trafficking system.
Project/Area Number |
23700389
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neuroscience in general
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Research Institution | Juntendo University |
Principal Investigator |
HATANO Taku 順天堂大学, 医学部, 准教授 (60338390)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | DJ-1 / 家族性パーキンソン病 / 膜輸送 / シナプトソーム / synaptophysin / Rab3A / Synaptophysin / FRET / Immunoisolation |
Research Abstract |
Parkinson's disease (PD) is a neurodegenerative disorder caused by loss of dopaminergic neurons. Although many reports have suggested that genetic factors are implicated in the pathogenesis of PD, molecular mechanisms underlying selective dopaminergic neuronal degeneration remain unknown. DJ-1 is a causative gene for autosomal recessive form of PARK7-linked early-onset PD. In this project, my colleagues and I revealed that DJ-1 distributes to the cytosol and membranous structures in a punctate appearance in cultured cells and in primary neurons obtained from mouse brain. Additionally, DJ-1 colocalizes with GM130, synaptophysin and Rab3A. Forster resonance energy transfer analysis revealed that a small portion of DJ-1 interacts with synaptophysin in living cells. Although the wild-type DJ-1 protein directly associates with membranes without an intermediary protein, the pathogenic L166P mutation of DJ-1 exhibits less binding to synaptic vesicles. These results indicate that DJ-1 associates with membranous organelles including synaptic membranes to exhibit its normal function.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] VPS35 mutation in Japanese patients with typical Parkinos's disease2012
Author(s)
Ando M, Funayama M, Li Y, Kashihara K, Murakami Y, Ishizu N, Toyoda C, Noguchi K, Hashimoto T, Nakano N, Sasaki R, Kokubo Y, Kuzuhara S, Ogaki K, Yamashita C, Yoshino H, Hatano T, Tomiyama H, Hattori N.
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Journal Title
Mov Disord
Volume: 27
Pages: 1413-7
Related Report
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[Journal Article] Young Japanese Expert Group for Parkison's Disease and Movement Disorders: YJ-EXPANDS. Validity and reliability assessment of a Japanese version of the Snaith-Hamilton pleasure scale2012
Author(s)
Nagayama H, Kubo S, Hatano T, Hamada S, Maeda T, Hasegawa T, Kadowaki T, Terashi H, Yoshioka M, Nomoto N, Kano O, Inoue M, Shimura H, Takahashi T, Uchiyama T, Watanabe H, Kaneko S, Takahashi T, Baba Y
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Journal Title
Intern Med
Volume: 51
Pages: 865-9
Related Report
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[Journal Article] LRRK2 I2020T mutation is associated with tau pathology2012
Author(s)
Ujiie S, Hatano T, Kubo SI, Imai S, Sato S, Uchihara T, Yagishita S, Hasegawa K, Kowa H, Sakai F, Hattori N.
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Journal Title
Parkinsonism Relat Disord
Volume: 18
Pages: 819-23
Related Report
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[Journal Article] Pramipexole reduces the prevalence of fatigue in patients with Parkinson's disease2011
Author(s)
Morita A, Okuma Y, Kamei S, Yoshii F, Yamamoto T, Hashimoto S, Utusmi H, Hatano T, Hattori N, Matsumura M, Takahashi K, Nogawa S, Watanabe Y, Miyamoto M, Hirata K.
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Journal Title
Intern Med
Volume: 50
Pages: 2163-8
NAID
Related Report
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[Journal Article] The effect of Ndrg2 expression on astroglial activation2011
Author(s)
Takeichi T, Takarada-Iemata M, Hashida K, Sudo H, Okuda T, Kokame K, Hatano T, Takanashi M, Funabe S, Hattori N, Kitamura O, Kitao Y, Hori O
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Journal Title
Neurochem International
Volume: 59
Pages: 21-7
NAID
Related Report
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[Journal Article] DJ-1 associates with synaptic membranes2011
Author(s)
Usami Y, Hatano T, Imai S, Kubo S, Sato S, Saiki S, Fujioka Y, Ohba Y, Sato F, Funayama M, Eguchi H, Shiba K, Ariga H, Shen J, Hattori N
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Journal Title
Neurobiol of Dis
Volume: 43
Pages: 651-62
Related Report
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[Journal Article] DJ-1 associates with synaptic membranes2011
Author(s)
Yukiko Usami, Taku Hatano, Satoshi Imai, Shin-ichiro Kubo, Shigeto Sato, Shinji Saiki, Yoichiro Fujioka, Yusuke Ohba, Fumiaki Sato, Manabu Funayama. Hiroto Eguchi, Kaori Shiba, Hiroyoshi Ariga, Jie Shen, Nobutaka Hattori
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Journal Title
Neurobiology of Disease
Volume: 43
Issue: 3
Pages: 651-662
DOI
Related Report
Peer Reviewed
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[Journal Article] The effect of Ndrg2 expression on astroglial activation.2011
Author(s)
Takeichi T, Takarada-Iemata M, Hashida K, Sudo H, Okuda T, Kokame K, Hatano T, Takanashi M, Funabe S, Hattori N, Kitamura O, Kitao Y, Hori O.
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Journal Title
Neurochemistry International
Volume: 59
Pages: 21-27
NAID
Related Report
Peer Reviewed
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