Analysis of the activity-dependent neurogenesis in the visual system of adult zebrafish

• Project/Area Number: 23700394
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Waseda University
• Principal Investigator: TANAKA Hideomi 早稲田大学, 日欧研究機構, 招聘研究員 (10392028)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経幹細胞 / adult neurogenesis / 神経科学 / 脳・神経 / 再生医学 / 発生・分化

Research Abstract

Gene expression in the adult zebrafish optic tectum after ennucleation was examined using zebrafish genome array(Affymetrix). 58 out of 89 genes (decrease group), 228 out of 320 genes (increase group), 30 out of 46 genes (intermediate group) were successfully annotated. 43 genes in the decrease group were subcloned. Most of candidate genes express ubiquitously in the optic tectum, and reproducible left-right asymmetry expression after ennucleation was not observed.

Research Products

• [Journal Article] Identification and expression analysis of the zebrafish homologs of the ceramide synthase gene family (2013)
  • Author(s): Brondolin M, Berger S, Reinke M, Tanaka H, Ohshima T, Fuβ B, Hoch M.
  • Journal Title: Dev. Dyn. Volume: 242 Pages: 189-200
  • Peer Reviewed
• [Journal Article] Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Tanaka H, Morimura R, Ohshima T.
  • Journal Title: Dev. Biol. Volume: 370 Pages: 223-236
  • Peer Reviewed
• [Journal Article] Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Hideomi Tanaka
  • Journal Title: Dev. Biol. Volume: 370 Issue: 2 Pages: 223-236
  • DOI: 10.1016/j.ydbio.2012.07.032
  • Peer Reviewed
• [Presentation] Setting up of zebrafish culture system in LIMES and current collaboration (2012)
  • Author(s): Hideomi Tanaka
  • Organizer: German-Japanese Joint Summer Symposium on Health Span Dynamics, The 12th Joint Symposium of Waseda University and University of Bonn
  • Place of Presentation: Bonn, Germany
• [Presentation] Dpysl2 (CRMP2),Dpysl3 (CRMP4), Cdk5 and DYRK2 are required for the positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Hideomi Tanaka, Rii Morimura, Toshio Ohshima
  • Organizer: 10th International Conference Zebrafish Development and Genetics
  • Place of Presentation: Madison,Wisconsin
• [Presentation] Phosphorylation of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) by Cdk5 and DYRK2 is required for the positioning of Rohon-beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Hideomi Tanaka, Rii Morimura, Toshio Ohshima
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Phosphorylation of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) by Cdk5 and DYRK2 is required for the positioning of Rohon-beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Hideomi Tanaka
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場, 福岡, 日本
• [Presentation] Setting up of zebrafish culture system in LIMES and current collaboration (2012)
  • Author(s): Hideomi Tanaka
  • Organizer: German-Japanese Joint Summer Symposium on Health Span Dynamics ,The 12th Joint Symposium of Waseda University and University of Bonn
  • Place of Presentation: Univ. Bonn, LIMES, Bonn, Germany
• [Presentation] Dpysl2(CRMP2),Dpysl3(CRMP4),Cdk5 and DYRK2 are required for the positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish (2012)
  • Author(s): Hideomi Tanaka
  • Organizer: 10th International Conference Zebrafish Development and Genetics
  • Place of Presentation: Univ. Wisconsin, Madison, Wisconsin, USA
• [Presentation] Role of Dpysl (CRMP) family in cellular integrity of roof plate and determination of the position of Rohon-Beard neuron (2011)
  • Author(s): Hideomi Tanaka
  • Organizer: German-Japanese Joint Symposium for Diamond Researchers
  • Place of Presentation: Bonn, Germany
• [Presentation] Adult neurogenesis in zebrafish optic tectum (2011)
  • Author(s): Hideomi Tanaka
  • Organizer: 2011 international joint meeting of Bonn University, Waseda University, University of Yamanashi and Tokyo University of Agriculture and Technology (TUAT)
  • Place of Presentation: Tokyo, Japan
• [Presentation] Role of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) in cellular integrity of roof plate and determination of the position of Rohon-Beard neuron (2011)
  • Author(s): Hideomi Tanaka, Rii Morimura, Hitoshi Okamoto, Toshio Ohshima
  • Organizer: 7th Europian Zebrafish Genetics and Development Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Analysis of neurogenesis in adult zebrafish optic tectum (2011)
  • Author(s): Hideomi Tanaka, Yoko Ito, Hitoshi Okamoto, Toshio Ohshima
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biologists
  • Place of Presentation: Dresden, Germany
• [Presentation] Role of Dpysl (CRMP) family in cellular integrity of roof plate and determination of the position of Rohon-Beard neuron (2011)
  • Author(s): Hideomi Tanaka
  • Organizer: German-Japanese Joint Symposium for Diamond Researchers
  • Place of Presentation: Univ. Bonn, Germany
• [Presentation] Role of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) in cellular integrity of roof plate and determination of the position of Rohon-Beard neuron (2011)
  • Author(s): Hideomi Tanaka, Rii Morimura, Hitoshi Okamoto, Toshio Ohshima
  • Organizer: 7th Europian Zebrafish Genetics and Development Meeting
  • Place of Presentation: Edinburgh International Conference Centre, Scotland
• [Presentation] Adult neurogenesis in zebrafish optic tectum (2011)
  • Author(s): Hideomi Tanaka
  • Organizer: 2011 international joint meeting of Bonn University, Waseda University, University of Yamanashi and Tokyo University of Agriculture and Technology (TUAT)
  • Place of Presentation: 東京農工大学、東京
• [Presentation] Gene expression analysis of the neural stem/progenitor cells in adult zebrafish optic tectum after eye removal (2011)
  • Author(s): Hideomi Tanaka, Yoko Ito, Hitoshi Okamoto, Toshio Ohshima
  • Organizer: 第44回 日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター、那覇
• [Presentation] Geneexpression analysis of the neural stem/progenitor cells in adult zebrafish optic tectum after eye removal (2010)
  • Author(s): Hideomi Tanaka, Yoko Ito, Hitoshi Okamoto, Toshio Ohshima
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 那覇
• [Remarks] 大島研究室 早稲田大学先進理工学部 生命医科学科
  • URL: http://www.ohshima.biomed.sci.waseda.ac.jp/