Molecular and cell biological analyses of mechanism of synaptic plasticity

• Project/Area Number: 23700395
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics
• Principal Investigator: KURUSU Mitsuhiko 国立遺伝学研究所, 構造遺伝学研究センター, 助教 (50413985)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Emiko 国立遺伝学研究所, 構造遺伝学研究センター, 准教授 (20173891)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: FGF / シナプス / 神経筋接合部 / ショウジョウバエ / 神経

Research Abstract

Although FGF signaling has been known to induce presynaptic differentiation in mammalian synaptic development, it remains unclear whether FGF signaling has a role in postsynaptic development. Here we report that Drosophila FGF signaling is required for the postsynaptic development of neuromuscular junctions. Two Drosophila FGF ligands, Pyramus and Thisbe, are expressed in neurons and muscles and a FGF receptor, Heartless, is localized in close proximity to postsynaptic GluRIIA clusters. Mutant analyses found that FGF signaling positively regulates G1uRI1A localization on postsynaptic sites. Moreover, GluRIIA expression is also controlled transcriptionally by FGF signaling. These results suggest that Drosophila FGF signaling is a regulator of Glutamate receptor expression in postsynaptic NMJ development.

Research Products

• [Journal Article] Developmental changes inexpression,subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation (2012)
  • Author(s): Kurusu, M., Katsuki, T., Zinn, K., Suzuki, E.
  • Journal Title: Developmental Biology Volume: 366 (2) Pages: 204-217
  • Peer Reviewed
• [Journal Article] Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation. (2012)
  • Author(s): Kurusu, M.,Katsuki, T., Zinn, K., Suzuki, E.
  • Journal Title: Developmental Biology Volume: 366 Issue: 2 Pages: 204-217
  • DOI: 10.1016/j.ydbio.2012.04.006
  • Peer Reviewed
• [Journal Article] Unc-51/ATG1 controls axonal and dendritic development via kines in-mediated vesicle transport in the Drosophila brain (2011)
  • Author(s): Mochizuki, H., Toda, H., Ando, M., Kurusu, M., Tomoda, T., Furukubo-Tokunaga, K.
  • Journal Title: PLoS One Volume: 6(5)
  • Peer Reviewed
• [Presentation] FGF signal ingregulates post synaptic development in the Drosophila neuromuscular junction (2012)
  • Author(s): 小林百合、来栖光彦、鈴木えみ子
  • Organizer: 日本ショウジョウバエ研究集会
  • Place of Presentation: 東京慈恵会医科大学
  • Year and Date: 2012-10-13
• [Presentation] ショウジョウバエFGFシグナルによる後シナプス分化の制御 (2012)
  • Author(s): 小林百合、来栖光彦、鈴木えみ子
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-19
• [Presentation] FGF signaling regulates post synaptic development in the Drosophila neuromuscular junction. (2012)
  • Author(s): 小林百合、来栖光彦
  • Organizer: The 10th Japanese Drosophila Research Conference
  • Place of Presentation: 東京慈恵医科大学
• [Presentation] Intrinsic control of spatiotemporal change in N-cadherin expression during neuronalmaturation (2011)
  • Author(s): 来栖光彦
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] Intrinsic control of spatiotemporal change in N-cadherin expression during neuronal maturation (2011)
  • Author(s): 来栖光彦
  • Organizer: 日本神経科学学会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
• [Remarks] 神経分化に伴う細胞接着分子N-cadherinの機能変化とその発現制御
  • URL: http://www.nig.ac.jp/Research-Highlights/992/1067.html