The analysis of cortical circuit in the awakening brain.

• Project/Area Number: 23700404
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SOHYA Kazuhiro 独立行政法人理化学研究所, 大脳皮質回路可塑性研究チーム, 研究員 (80415207)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 二光子レーザー走査型顕微鏡 / カルシウムイメージング / 大脳皮質視覚野 / 方位(傾き)選択性 / in vivo(6)光反応 / 蛍光タンパク質 / GABAergic Neuron(抑制性神経細胞) / 二光子励起 / GFP / GABAニューロン / 覚醒脳 / 方位選択性 / 眼優位可塑性 / ＧＦＰ / in vivo

Research Abstract

In this study, I applied in vivo two-photon functional calcium imaging of VGAT-Venus transgenic rats/mice, in which GABAergic neurons express Venus, a yellow fluorescent protein to the unanesthetized condition. And I analyzed the difference visual response properties between excitatory and inhibitory neurons in the awakening brain function

Research Products

• [Journal Article] Reciprocal Homosynaptic and Heterosynaptic Long-Term Plasticity of Corticogeniculate Projection Neurons in Layer VI of the Mouse Visual Cortex (2013)
  • Author(s): Arami MK, Sohya K, Sarihi A, Jiang B, Yanagawa Y, Tsumoto T
  • Journal Title: The Journal of Neuroscience Volume: (33) Pages: 7787-7798
  • Peer Reviewed
• [Journal Article] Cell type-specific, presynaptic LTP of inhibitory synapses on fast-spiking GABAergic neurons in the mouse visual cortex (2012)
  • Author(s): Sarihi A, Mirnajafi-Zadeh J, Jiang B, Sohya K, Safari MS, Arami MK, Yanagawa Y, Tsumoto T
  • Journal Title: The Journal of Neuroscience Volume: (32) Pages: 13189-13199
  • Peer Reviewed
• [Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδtrans-synaptic interaction (2012)
  • Author(s): Takahashi H, Katayama K, Sohya K, Miyamoto H, Prasad T, Matsumoto Y, Ota M, Yasuda H, Tsumoto T, Aruga J, Craig AM
  • Journal Title: Nature Neuroscience Volume: (15) Pages: 389-398
  • Peer Reviewed
• [Journal Article] Cell type-specific, presynaptic LTP of inhibitory synapses on fast-spiking GABAergic neurons in the mouse visual cortex. (2012)
  • Author(s): Sarihi A, Mirnajafi-Zadeh J, Jiang B, Sohya K, Safari MS, Arami MK, Yanagawa Y, Tsumoto T.
  • Journal Title: The Journal of Neuroscience Volume: 32 Pages: 13189-13199
  • Peer Reviewed
• [Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδ trans-synaptic interaction (2012)
  • Author(s): Hideto Takahashi, Kei-ichi Katayama, Kazuhiro Sohya, Hiroyuki Miyamoto, Tuhina Prasad, Yoshifumi Matsumoto, Maya Ota, Hiroki Yasuda, Tadaharu Tsumoto, Jun Aruga, Ann Marie Craig
  • Journal Title: Nature Neuroscience Volume: 15 Issue: 3 Pages: 389-398
  • DOI: 10.1038/nn.3040
  • Peer Reviewed
• [Presentation] Long-term synaptic plasticity at cortico-geniculate projection neurons in layer VI of the mouse visual cortex (2012)
  • Author(s): Masoumeh kourosh Arami, Abdolrahman Sarihi, Bin Jiang, Kazuhiro Sohya, Shu-Ting Yin, Tadaharu Tsumoto
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2012-09-21
• [Presentation] Comparison of neural activity in the visual cortex of rats between in awake and anesthetized conditions by two-photon functional imaging (2012)
  • Author(s): Rui Kimura, Kazuhiro Sohya, Hirofumi Ozeki, Teppei Ebina, Yuchio Yanagawa, Tadaharu Tsumoto
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2012-09-18
• [Presentation] 二光子励起機能的カルシウムイメージング法を用いた大脳皮質視覚野神経回路網の解析 (2012)
  • Author(s): 惣谷和広
  • Organizer: 第37回日本睡眠学会定期学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-29
• [Presentation] 二光子顕微鏡を用いた覚醒、麻酔下における視覚応答特性の比較 (2012)
  • Author(s): 木村 塁、惣谷 和広、尾関 宏文、蝦名 鉄平、柳川 右千夫、津本 忠治
  • Organizer: 日本神経科学学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Long-term synaptic plasticity at cortico-geniculate projection neurons in layer VI of the mouse visual cortex (2012)
  • Author(s): Masoumeh kourosh Arami、Abdolrahman Sarihi、Bin Jiang、Kazuhiro Sohya、Shu-Ting Yin、Tadaharu Tsumoto
  • Organizer: 日本神経科学学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 二光子例機能的カルシウムイメージング法を用いた大脳皮質視覚野神経回路網の解析 (2012)
  • Author(s): 惣谷和広
  • Organizer: 日本睡眠学会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] Mechanism underlying LTP of inhibitory synapses to GABAergic neurons in layer II/III of the mouse visual cortex (2011)
  • Author(s): Abdolrahman Sarihi, Javad Mirnajafi-Zadeh, Bin Jiang, Kazuhiro Sohya, Yuchio Yanagawa,Tadaharu Tsumoto
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Year and Date: 2011-09-17
• [Presentation] Three-dimensional analysis of visually responsive Parvalbumin-positive neurons in the mouse visual cortex (2011)
  • Author(s): Teppei Ebina, Kazuhiro Sohya, Rui Kimura, Yin Shu-Ting, Hirofumi Ozeki, Yuchio Yanagawa, Hiroshi Kameda, Takeshi Kaneko, Tadaharu Tsumoto
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2011-09-16
• [Presentation] Homosynaptic LTP and heterosynaptic LTD in layer VI of the mouse visual cortex induced by white matter or layer II/III stimulation (2011)
  • Author(s): Masoumeh Kourosh Arami, Abdorahman Sarihi, Bin Jiang, Kazuhiro Sohya, Tadaharu Tsumoto
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2011-09-15
• [Presentation] 二光子励起機能的Ca2+イメージング法を用いた大脳皮質視覚野神経回路網の解析 (2011)
  • Author(s): 惣谷和広
  • Organizer: 第一回睡眠研究会
  • Place of Presentation: 岡崎カンファレンスセンター
• [Presentation] Homosynaptic LTP and heterosynaptic LTD in layer VI of the mouse visual cortex induced by white matter or layer II/III stimulation (2011)
  • Author(s): Masoumeh Kourosh Arami, Abdorahman Sarihi, Bin Jiang, Kazuhiro Sohya, Tadaharu Tsumoto
  • Organizer: 日本神経科学学会大会（Neuroscience2011）
  • Place of Presentation: パシフィコ横浜
• [Presentation] マウス大脳皮質視覚野における視覚応答性Parvalbumin陽性ニューロンクラスターの三次元解析 (2011)
  • Author(s): 蝦名 鉄平、 惣谷 和広、 木村 塁、 Yin Shu-Ting、 尾関 宏文、 柳川 右千夫、 亀田 浩司、 金子 武嗣、 津本 忠治
  • Organizer: 日本神経科学学会大会（Neuroscience2011）
  • Place of Presentation: パシフィコ横浜
• [Presentation] マウス視覚野2/3層のGABAニューロンへの抑制性シナプス長期増強のメカニズム (2011)
  • Author(s): Abdolrahman Sarihi、 Javad Mirnajafi-Zadeh、 Bin Jiang、 惣谷 和広、 柳川 右千夫、 津本 忠治
  • Organizer: 日本神経科学学会大会（Neuroscience2011）
  • Place of Presentation: パシフィコ横浜
• [Presentation] 二光子励起機能的Ca2+イメージング法を用いた大脳皮質視覚野神経回路網の解析 (2011)
  • Author(s): 惣谷和広
  • Organizer: 第一回睡眠研究会(招待講演)
  • Place of Presentation: 岡崎コンファレンスセンター
• [Book] 生物物理 (2012)
  • Author(s): 惣谷和広、蝦名鉄平、津本忠治
• [Book] 生物物理 (2012)
  • Author(s): 惣谷和広、蝦名鉄平、津本忠治
  • Total Pages: 6
  • Publisher: 生物物理学会