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Functional analysis of the spinal motor circuits in zebrafish using optogenetics

Research Project

Project/Area Number 23700408
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neuroscience in general
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

KIMURA Yukiko  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特別協力研究員 (70581122)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords光遺伝学 / ゼブラフィッシュ / ロコモーション / 神経科学
Research Abstract

Optogenetics is a innovative technique which allows us to control neural activity of specific neurons by light. In this research, I planned to apply optogenetics to analyzing function of the spinal motor circuits in zebrafish. First, I succeeded in generating several transgenic zebrafish expressing optogenetic molecules in specific neurons. Next, I tried to analyze the function of several spinal interneurons by using of these transgenic fish. However, this analysis was difficult to perform due to some technical limitations. Instead of the spinal cord, I analyzed specific neurons in the hindbrain using optogenetics. I identified a group of neurons which is indispensable to swimming behavior. This results is important for understanding the neural circuits controlling locomotion in vertebrates.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] 光遺伝学ツールを用いたゼブラフィッシュの行動制御2014

    • Author(s)
      木村有希子、東島眞一
    • Journal Title

      細胞工学

      Volume: 33(3) Pages: 255-259

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons2013

    • Author(s)
      Satou, C., Kimura, Y., Hirata, H., Suster, M.L, Kawakami, K., and Higashijima, S.
    • Journal Title

      Development

      Volume: 140 Issue: 18 Pages: 3927-3931

    • DOI

      10.1242/dev.099531

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hindbrain V2a neurons in the excitation of spinal locomotor circuits during zebrafish swimming2013

    • Author(s)
      Kimura, Y., Satou, C., Fujioka, S., Shoji, W., Umeda, K., Ishizuka, T., Yawo, H., and Higashijima, S.
    • Journal Title

      Current Biology

      Volume: 23 Issue: 10 Pages: 843-849

    • DOI

      10.1016/j.cub.2013.03.066

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] オプトジェネティックツールを用いたゼブラフィッシュ運動系神経回路の解析2012

    • Author(s)
      東島眞一・木村有希子
    • Journal Title

      実験医学

      Volume: 30(16) Pages: 2588-2589

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Generation of Multiple Classes of V0 Neurons in Zebrafish Spinal Cord: Progenitor Heterogeneity and Temporal Control of Neuronal Diversity2012

    • Author(s)
      Chie Satou, Yukiko Kimura, Shin-ichi Higashijima
    • Journal Title

      The Journal of Neuroscience

      Volume: 32(5) Issue: 5 Pages: 1771-1783

    • DOI

      10.1523/jneurosci.5500-11.2012

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Hindbrain V2a neurons play critical roles in providing excitations to zebrafish spinal locomotor circuits during swimming2013

    • Author(s)
      Kimura Y, Satou C, Fujioka S, Shoji W, Umeda K, Ishizuka T, Yawo H, Higashijima S
    • Organizer
      JSCPB2013
    • Place of Presentation
      兵庫県イーグレひめじ
    • Related Report
      2013 Final Research Report
  • [Presentation] Hindbrain V2a neurons play critical roles in providing excitations to zebrafish spinal locomotor circuits during swimming2013

    • Author(s)
      Kimura Y, Satou C, Fujioka S, Shoji W, Umeda K, Ishizuka T, Yawo H, Higashijima S
    • Organizer
      JSCPB2013
    • Place of Presentation
      イーグレひめじ(兵庫県姫路市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Chx10-positive neurons in the hindbrain play essential roles during swimming in zebrafish2012

    • Author(s)
      Kimura Y, Satou C, Higashijima S
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      愛知県名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] ゼブラフィッシュ遊泳行動における後脳Chx10ニューロンの機能解析2012

    • Author(s)
      木村有希子、東島眞一
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪府大阪大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Chx10-positive neurons in the hindbrain play essential roles during swimming in zebrafish2012

    • Author(s)
      Kimura Y, Satou C, Higashijima S
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      愛知県 名古屋国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] ゼブラフィッシュ遊泳行動における後脳Chx10ニューロンの機能解析2012

    • Author(s)
      木村有希子,東島眞一
    • Organizer
      日本動物学会第83 回大会
    • Place of Presentation
      大阪府 大阪大学
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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