The role of FGF signal in brain development

• Project/Area Number: 23700410
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Chiba University
• Principal Investigator: SATO Tatsuya 千葉大学, 大学院・医学研究院, 助教 (00568222)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経発生 / 形態形成 / 神経回路形成 / 脳 / 神経 / 発生 / 神経回路

Research Abstract

It was shown that the combination of in vivo electroporation andthe newest version of the tetracycline (Tet)-controlled gene regulatory (Tet-On) systemefficiently can induce gene expression in various types of neurons such as cerebralpyramidal neurons in mouse embryonic and postnatal tissues. Fate map analysis showedthat the Fgf8-expressing cells differentiate into the cerebellum. The marked cells aredetected in more posterior and lateral part of the cerebellum when the Fgf8-expressingcells are marked in earlier developmental stage.

Research Products

• [Journal Article] Inducible gene expression in postmitotic neurons by an in vivo electroporation-based tetracycline system (2013)
  • Author(s): Sato, T., Muroyama, Y. and Saito, T
  • Journal Title: Journal of Neuroscience Methods Volume: 214 Issue: 2 Pages: 170-176
  • DOI: 10.1016/j.jneumeth.2013.01.014
  • Peer Reviewed
• [Presentation] Nepro is required for the maintenance of neural progenitor cells in the early neocortex (2012)
  • Author(s): Muroyama, Y., Sato, T. and Saito, T
  • Organizer: Neocortical Organization, 1st International Symposium/59th NIBB Conference
  • Place of Presentation: 岡崎カンファレンスセンター、岡崎
  • Year and Date: 2012-03-12
• [Presentation] Nepro is Required for the Maintenance of Neural Progenitor Cells in the Early Neocortex (2012)
  • Author(s): Saito, T.
  • Organizer: Neocortical Organization, 1st International Symposium/59th NIBB Conference
  • Place of Presentation: 岡崎カンファレンスセンター（岡崎）
• [Presentation] Functional analysis of Nepro, a gene required for the maintenance of neocortex neural progenitor cells (2011)
  • Author(s): Sato, T., Muroyama, Y. and Saito, T
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2011-09-16
• [Presentation] Functional analysis of Nepro, a gene required for the maintenance of neocortex neural progenitor cells (2011)
  • Author(s): Sato, T.
  • Organizer: 日本神経科学大会
  • Place of Presentation: パシフィコ横浜（横浜）