Simultaneous visualization of visual responses and mono-synaptic circuits
| Project/Area Number |
23700419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
MORI Takuma 生理学研究所, 生体情報研究系, 助教 (70545798)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 神経回路網 / 神経トレーサー / ウイルスベクター / 神経回路 / 視覚 |
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| Research Abstract |
I developed a new glycoprotein-deleted rabies virus based on a human vaccine strain, HEP-Flury. I visualized monosynaptic neuronal circuits of neocortical layer 2/3 pyramidal neurons using the virus. I electroporated plasmids into pyramidal cells in cortical layer 2/3 of mouse and injected EnvA-pseudotyped rabies virus. These neurons receive direct synaptic inputs from layers 2/3, 4 and 5. The virus enabled to observe the detail of the neuronal morphology. I analyzed the morphological characteristics of these presynaptic neurons.
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Report
(3 results)
Research Products
(6 results)
