Intracellular transport of DISC1/Neuregulin-1 and schizophrenia

• Project/Area Number: 23700443
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University
• Principal Investigator: MORI Daisuke 名古屋大学, 医学系研究科, COE 特任講師 (00381997)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 分子 / 細胞 / 神経生物学 / 統合失調症 / DISC1 / ニューレグリン / Neuregulin-1

Research Abstract

We hypothesized that DISC1 would be involved in vesicular transport of various proteins and mRNAs which maintain neuronal function. Previously, we have identified direct interaction of DISC1 and Neuregulin-1, which is a ligand of ErbB3 and ErbB4. To understand the sense of their association, we have attempted to analyze their common interacting proteins. As a result of the comprehensive screenings, we have identified AP family proteins, which have essential roles for intracellular vesicle trafficking. Moreover, we have established a new experimental system that enables us to quantify the secretion of processed Neuregulin-1 by immunoblotting. We found that the processed Neuregulin-1 was decreased in cultured hippocampal neuron from DISC1-deficient mice. 交付決定額

Research Products

• [Journal Article] Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse (2011)
  • Author(s): 黒田啓介、森大輔、貝淵弘三 他21名
  • Journal Title: Human Molecular Genetics誌 Volume: 20巻 Pages: 4666-4683
  • Peer Reviewed
• [Journal Article] Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse (2011)
  • Author(s): K Kuroda, S Yamada, M Tanaka, M Iizuka, H Yano, D Mori, D Tsuboi, T Nishioka, T Namba, Y Iizuka, S Kubota, T Nagai, D Ibi, R Wang, A Enomoto, M Isotani-Sakakibara, N Asai, K Kimura, H Kiyonari, T Abe, A Mizoguchi,M Sokabe, M Takahashi, K Yamada, K Kaibuchi
  • Journal Title: Human molecular genetics Volume: 20 Issue: 23 Pages: 4666-4683
  • DOI: 10.1093/hmg/ddr400
  • Peer Reviewed
• [Presentation] Molecular regulation of pro-Neuregulin intracellular trafficking (2012)
  • Author(s): 森大輔、貝淵弘三
  • Organizer: ニューレグリン前駆体の細胞内輸送機構ポスター演題日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-18
• [Presentation] ニューレグリン前駆体の細胞内輸送 (2012)
  • Author(s): 森大輔
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋国際会議場（名古屋）