Chromatin Dynamics Associated with Neuronal Differentiation

• Project/Area Number: 23700447
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Osaka University
• Principal Investigator: SUGO Noriyuki 大阪大学, 大学院・生命機能研究科, 助教 (20372625)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 発生・分化・老化 / DNA 修復 / 1分子生理・生化学 / 神経科学 / 脳・神経 / 発生・分化 / 発現制御 / ナノバイオ

Research Abstract

Neuronal differentiation during cortical development is regulated by dynamics of chromatin containing DNA and transcription factors. Here we examined spatiotemporalinteractions between fluorescent-tagged transcription factors and their target sites in living cells using single-molecule imaging techniques. The transcription factor binding to its target sequences showed longer residence time compared to that binding to unrelated sequences in the nucleus.

Research Products

• [Presentation] Single-Molecule Imaging Reveals Dynamics of CREB Transcription Factor in Living Cells (2013)
  • Author(s): Noriyuki Sugo, Masatoshi Morimatsu, Yoshiyuki Arai, Yoshinori Kousoku, Aya Ohkuni, Taishin Nomura, Toshio Yanagida, Nobuhiko Yamamoto
  • Organizer: The 23rdCDB Meeting Building multi cellular systems from Cellular Cross-Talk
  • Place of Presentation: 神戸
  • Year and Date: 2013-01-22
• [Presentation] 山本亘彦1分子イメージングによる転写因子 CREB の動態解析 (2012)
  • Author(s): 菅生紀之、森松賢順、新井由之、香束剛章、大國紋、野村泰伸、柳田敏雄
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Single-Molecule Imaging of Transcription Factor CREB in Living Cells (2012)
  • Author(s): Noriyuki Sugo, Masatoshi Morimatsu, Yoshiyuki Arai, Yoshinori Kousoku, Aya Ohkuni, Taishin Nomura, Toshio Yanagida, Nobuhiko Yamamoto
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-15
• [Presentation] 1分子イメージングによる転写因子 CREBの核内動態解析 (2012)
  • Author(s): 菅生紀之、森松賢順、新井由之、香束剛章、大國紋、野村泰伸、柳田敏雄、山本亘彦
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-09
• [Presentation] Histone deacetylase 9 (HDAC9) regulates thalamocortical axon branching by shuttling from the nucleus to cytoplasm in an activity-dependent fashion (2011)
  • Author(s): Alchini, R., Sugo, N., Yamamoto, N.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] Nucleocytoplasmic translocation of the histone deacetylase 9 (HDAC9) regulates thalamocortical axon branching (2011)
  • Author(s): Alchini, R., Sugo, N., Yamamoto, N.
  • Organizer: Cortical Development
  • Place of Presentation: CHANIA, GREECE
• [Presentation] Histone deacetylase 9 (HDAC9) regulates thalamocortical axon branching by shuttling from the nucleus to cytoplasm in an activity-dependent fashion (2011)
  • Author(s): Alchini, R., Sugo, N., Yamamoto, N.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Nucleocytoplasmic translocation of the histone deacetylase 9 (HDAC9) regulates thalamocortical axon branching (2001)
  • Author(s): Alchini, R., Sugo, N., Yamamoto, N
  • Organizer: Cortical Development
  • Place of Presentation: Chania, Greece
  • Year and Date: 2001-05-20
• [Presentation] １分子イメージングによる転写因子CREBの核内動態解析
  • Author(s): 菅生紀之
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Single-molecule imaging of transcription factor CREB in living cells
  • Author(s): Noriyuki Sugo
  • Organizer: Neuroscience2012
  • Place of Presentation: New Orleans, USA
• [Presentation] １分子イメージングによる転写因子CREBの動態解析
  • Author(s): 菅生紀之
  • Organizer: 第３５回分子生物学会年会
  • Place of Presentation: 福岡
• [Remarks] 大阪大学大学院生命機能研究科細胞分子神経生物学研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks] 大阪大学大学院生命機能研究科細胞分子神経生物学研究室（山本研究室）
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/