Mode of action of Septin-molecular complex
Project/Area Number |
23700463
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Nagoya University |
Principal Investigator |
SHINODA Tomoyasu 名古屋大学, 医学(系)研究科(研究院), 助教 (80505652)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 神経発生 / 大脳皮質形成 / 大脳皮質発生 |
Research Abstract |
Septins are a family of conserved GTP/GDP-binding proteins implicated in a variety of cellular functions such as cell cycle control and cytokinesis. We performed a screening to indentify interacting molecules for Sept4 and Sept14. By using a method combining affinity column chromatography with shotgun liquid chromatography tandem mass spectrometry, we obtained several candidates including Drebrin-1 and Cofilin-1, regulator proteins of actin cytoskeleton. As it was previously reported that Cofilin-1 is involved in cortical neuronal migration, we examined the functional involvement of Drebrin-1 for neuronal migration. Knockdown experiments by in utero electroporation showed that reduction of Drebrin-1 caused inhibition of neuronal migration. Biochemical analyses revealed the direct interaction between Sept4 and Drebrin-1, Sept14 and Cofilin-1, respectively. These results suggest that Sept4-Sept14 complex involved in neuronal migration by regulating the dynamics of actin cytoskeleton.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.2013
Author(s)
Okamoto M, Namba T, Shinoda T, Kondo T, Watanabe T, Inoue Y, Takeuchi K, Enomoto Y, Ota K, Oda K, Wada Y, Sagou K, Saito K, Sakakibara A, Kawaguchi A, Nakajima K, Adachi T, Fujimori T, Ueda M, Hayashi S, Kaibuchi K, Miyata T
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Journal Title
Nature Neuroscience
Volume: 16
Issue: 11
Pages: 1556-66
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 神経上皮偽重層化構造の維持機構2014
Author(s)
Tomoyasu Shinoda, Ryo Higuchi, Yugo Fukazawa, Mayumi Okamoto, Arata Nagasaka, Kumiko Ota, Takashi Miura, Masaharu Nagayama, Toshihiko Fujimori, Takaki Miyata
Organizer
第七回神経発生討論会
Place of Presentation
大阪大学吹田キャンパス、大阪府
Related Report
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