| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Septins are a family of conserved GTP/GDP-binding proteins implicated in a variety of cellular functions such as cell cycle control and cytokinesis. We performed a screening to indentify interacting molecules for Sept4 and Sept14. By using a method combining affinity column chromatography with shotgun liquid chromatography tandem mass spectrometry, we obtained several candidates including Drebrin-1 and Cofilin-1, regulator proteins of actin cytoskeleton. As it was previously reported that Cofilin-1 is involved in cortical neuronal migration, we examined the functional involvement of Drebrin-1 for neuronal migration. Knockdown experiments by in utero electroporation showed that reduction of Drebrin-1 caused inhibition of neuronal migration. Biochemical analyses revealed the direct interaction between Sept4 and Drebrin-1, Sept14 and Cofilin-1, respectively. These results suggest that Sept4-Sept14 complex involved in neuronal migration by regulating the dynamics of actin cytoskeleton.
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