Detecting mechanism of tastant concentrations by a kinetic model for action potentials and generator potentials.

• Project/Area Number: 23700469
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: OHTUBO Yoshitaka 九州工業大学, 生命体工学研究科(研究院), 准教授 (00380725)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 味蕾細胞 / 活動電位 / 電位依存性チャネル / パッチクランプ法 / single cell RT-PCR / 免疫染色法 / 免疫染色 / リアルタイムPCR

Research Abstract

Action potentials in taste receptor cells play an important role in taste transmissions to neighboring cells including taste nerve fibers. I show that the shape of action potentials differs among cell types and that taste bud cells express voltage-gated channels and their genes in a cell-type-dependent manner. The discussion is made on the role of these voltage-gated currents in the transmission of taste responses.

Research Products

• [Journal Article] Cell-type-dependent action potentials and voltage-gated currents in mouse fungiform taste buds (2014)
  • Author(s): Kimura K, Ohtubo Y, Tateno K, Takeuchi K, Kumazawa T, Yoshii K.
  • Journal Title: European Journal of Neuroscience Volume: 39(1) Pages: 24-34
• [Journal Article] Cell-type-dependent action potentials and voltage-gated currents in mouse fungiform taste buds. (2014)
  • Author(s): Kimura K, Ohtubo Y, Tateno K, Takeuchi K, Kumazawa T, Yoshii K.
  • Journal Title: European Journal of Neuroscience Volume: 39(1) Issue: 1 Pages: 24-34
  • DOI: 10.1111/ejn.12388
  • Peer Reviewed
• [Journal Article] Subtype-dependent postnatal development of taste receptor cells in mouse fungiform taste buds. (2012)
  • Author(s): Ohtubo Y, Iwamoto M, Yoshii K.
  • Journal Title: European Journal of Neuroscience Volume: 未定 Issue: 11 Pages: 1661-1671
  • DOI: 10.1111/j.1460-9568.2012.08068.x
  • Peer Reviewed
• [Journal Article] Network model of chemical-sensing system inspired by mouse taste buds. (2011)
  • Author(s): Tateno K, Igarashi J, Ohtubo Y, Nakada K, Miki T, Yoshii K.
  • Journal Title: Biological Cybernetics Volume: 105 (1) Issue: 1 Pages: 21-27
  • DOI: 10.1007/s00422-011-0447-5
  • Peer Reviewed
• [Presentation] Action potential shapes for a change in TEA-insensitive current ratio in mouse taste bud cell model (2013)
  • Author(s): Tateno K, Ohtubo Y, Kimura K, Takeuchi K, Kumazawa T, Yoshii K.
  • Organizer: FENS Featured Regional Meeting
  • Place of Presentation: Prague, Czech Republic(Final Programme p50, PI-D-105)
• [Presentation] Aging effects on electrophysiological properties of taste receptor cells in ~20-month-old mice (2013)
  • Author(s): Takeuchi K, Ohtubo Y, Yoshii K.
  • Organizer: FENS Featured Regional Meeting
  • Place of Presentation: Prague, Czech Republic(Final Programme p50, PI-D-106)
• [Presentation] Postnatal development of taste buds in mouse soft palates (2013)
  • Author(s): Ohtubo Y, Hashiguchi Y, Yamasaki T, Yoshii K.
  • Organizer: The 47th Annual Meeting of the Japanese Association for the Study of Taste and Smell
  • Place of Presentation: Sendai, Japan(Program and Abstracts, p 86, P-011)
• [Presentation] Aging of voltage-gated currents in mouse fungiform taste bud cells (2013)
  • Author(s): Ohtubo Y, Takeuchi K, Yoshii K.
  • Organizer: The Journal of Physiological Sciences, Proceedings of the 90th Annual Meeting
  • Place of Presentation: Tokyo, Japan(Volume 63, supplement 1, p S253, 3PK-079)
• [Presentation] Action potential shapes for a change in TEA-insensitive current ratio in mouse taste bud cell model. (2013)
  • Author(s): Tateno K, Ohtubo Y, Kimura K, Takeuchi K, Kumazawa T, Yoshii K.
  • Organizer: FENS Featured Regional Meeting
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Aging effects on electrophysiological properties of taste receptor cells in ~20-month-old mice. (2013)
  • Author(s): Takeuchi K, Ohtubo Y, Yoshii K.
  • Organizer: FENS Featured Regional Meeting
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Postnatal development of taste buds in mouse soft palates. (2013)
  • Author(s): Ohtubo Y, Hashiguchi Y, Yamasaki T, Yoshii K.
  • Organizer: The 47th Annual Meeting of the Japanese Association for the Study of Taste and Smell
  • Place of Presentation: Sendai, Japan
• [Presentation] Aging of voltage-gated currents in mouse fungiform taste bud cells. (2013)
  • Author(s): Y. Ohtubo, K. Takeuchi, K. Yoshii
  • Organizer: The Journal of Physiological Sciences, Proceedings of the 90th Annual Meeting
  • Place of Presentation: Tokyo, Japan
• [Presentation] Cell type dependence of voltage-gated sodium channel currents in fungiform taste bud cells (2012)
  • Author(s): Ohtubo Y, Yoshii K.
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain(Programme p184)
• [Presentation] Action potentials and voltage-gated currents of mouse taste bud cells (2012)
  • Author(s): Yoshii K, Kimura K, Ohtubo Y, Kumazawa T.
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain(Programme p184)
• [Presentation] Cell type dependence of voltage-gated sodium channel currents in fungiform taste bud cells. (2012)
  • Author(s): Y. Ohtubo, K. Yoshii
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain
• [Presentation] Action potentials and voltage-gated currents of mouse taste bud cells. (2012)
  • Author(s): K. Yoshii, K. Kimura, Y. Ohtubo, K. Kumazawa
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain
• [Presentation] Bullfrog taste nerve responses under hyperosmotic conditions. (2012)
  • Author(s): T. Kumazawa, K. Mashiyama, Y. Ohtubo, K. Yoshii
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain
• [Presentation] Postnatal development of taste bud cells in mouse fungiform papillae (2011)
  • Author(s): Ohtubo Y, Iwamoto M, Yoshii K.
  • Organizer: 8th International Brain Research Organization (IBRO) World congress of neuroscience
  • Place of Presentation: Florence, Italy(Program book p118, C242)
• [Presentation] Postnatal development of taste bud cells in mouse fungiform papillae. (2011)
  • Author(s): Ohtubo Y, Iwamoto M, Yoshii K.
  • Organizer: 8th International Brain Research Organization (IBRO) World congress of neuroscience
  • Place of Presentation: Florence, Italy
• [Presentation] Selective expression of a muscarinic acetylcholine receptor M3 on type III cells in mouse fungiform taste buds. (2011)
  • Author(s): Mori Y, Ohtubo Y, Yoshii K.
  • Organizer: 8th International Brain Research Organization (IBRO) World congress of neuroscience
  • Place of Presentation: Florence, Italy