Modulation by proteinase-activated receptor of TRP regulation of nociceptive transmission in the adult rat spinal dorsal horn
| Project/Area Number |
23700470
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | TRPチャネル / 膠様質 / 興奮性シナプス伝達 / 抑制性シナプス伝達 / プロテアーゼ受容体 / 植物由来物質 / パッチクランプ法 / 痛覚情報伝達 / 神経生理学 / 脊髄 / パッチクランプ / 脊髄後角 |
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| Outline of Final Research Achievements |
The spinal dorsal horn lamina II (substantia gelatinosa; SG) plays an important role in the modulation of nociceptive transmission. Some plant-derived chemical compounds are known to activate the TRP channels in the periphery. In the present study, their effects on synaptic transmission in SG neurons were examined by using the whole-cell patch-clamp technique. As a result, it was revealed that piperine (derived form black pepper), (-)-carvone (derived form spearmint), 1,4-cineole (derived form eucalyptus) increase the frequency of spontaneous excitatory postsynaptic current (EPSC) by activating TRPV1 in a reversible and concentration-dependent manner. On the other hand, eugenol (derived form laurel), zingerone (derived form ginger), carvacrol (derived form oregano) (+)-carvone (derived form caraway), 1,8-cineole (derived form eucalyptus) increased the frequency of spontaneous EPSC by activating TRPA1 in a reversible and concentration-dependent manner.
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Report
(5 results)
Research Products
(163 results)
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[Presentation] Inhibition by various aroma-oil chemicals of compound action potentials in the frog sciatic nerve.2013
Author(s)
Sena Ohtsubo, Akitomo Matsushita, Tsugumi Fujita, Hiroki Kawasaki, Chang-Yu Jiang, Qing-Tian Luo, Qin Kang, Sadahiko Masuko, Eiichi Kumamoto
Organizer
Neuroscience 2013 - Society for Neuroscience the 43rd Annual Meeting
Place of Presentation
The San Diego Convention Center (San Diego, CA, USA)
Related Report
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[Presentation] 1,8-および1,4-シネオールは成熟ラット脊髄膠様質ニューロンのグルタミン酸作動性の自発性興奮性シナプス伝達を促進する.2013
Author(s)
藤田亜美, 徐 年香, 蒋 昌宇, 羅 清甜, 康 欽, 八坂敏一, 大坪瀬奈, 松下晋大, 熊本栄一
Organizer
第36回日本神経科学大会
Place of Presentation
国立京都国際会館 (京都府京都市)
Related Report
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