Induction of oligodendrocytes from mouse iPS cells for transplantationinto white matter injury and association with physiological low oxygen

• Project/Area Number: 23700471
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Nagoya City University
• Principal Investigator: MISUMI Sachiyo 名古屋市立大学, 大学院・医学研究科, 助教 (70529148)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: オリゴデンドロサイト / iPS 細胞 / 低酸素 / 細胞移植 / 無血清培養 / 脳室周囲白質障害 / ｵﾘｺﾞﾃﾞﾝﾄﾞﾛｻｲﾄ / iPS細胞 / マウスiPS細胞 / 磁気ビーズ

Research Abstract

To obtain appropriate donor cells for transplantation, we differentiated oligodendrocyte (OL) lineage cells from mouse iPS cells. iPS cell-derived oligodendrocyte precursor cells (OPC) were induced effectively under serum free condition by 7-step culture method. Then we removed SSEA-1 (+) cells with MACS to prevent teratoma formation, resulting in 97% reduction. Physiological low oxygen promoted OPC proliferation with no effects for cell differentiation in vitro. iPS-derived OPCs survived in immature rat brain after transplantation, suggesting these cells are suitable for donor cells.

Research Products

• [Journal Article] Differentiation of oligodendrocytes from mouse induced pluripotent stem cells without serum (2013)
  • Author(s): Misumi S, Nishigaki R, Ueda Y, Watanabe Y, Shimizu Y, Ishida A, Jung CG, Hida H
  • Journal Title: Transl Stroke Res Volume: 4(2) Issue: 2 Pages: 149-157
  • DOI: 10.1007/s12975-012-0250-1
  • Peer Reviewed
• [Journal Article] Differentiation of oligodendrocytes from mouse induced pluripotent stem cells without serum. (2013)
  • Author(s): Sachiyo Misumi
  • Journal Title: Translational Stroke Reserach Volume: 4 Pages: 149-157
  • Peer Reviewed
• [Journal Article] Minor neuronal damage and recovered cellular proliferation in the hippocampus after continuous unilateral forelimb restraint in normal rats (2011)
  • Author(s): Akimasa Ishida, Yoshitomo Ueda, Kazuto Ishida, Sachiyo Misumi, Tadashi Masuda, Masataka Fujita, Hideki Hida
  • Journal Title: J Neurosc Res Volume: 89 Issue: 3 Pages: 457-465
  • DOI: 10.1002/jnr.22566
  • Peer Reviewed
• [Presentation] 障害モデルへの細胞移植に向けたマウス iPS 細胞由来オリゴデンドロサイト前駆細胞の分化誘導 (2013)
  • Author(s): 三角吉代、鄭且均、飛田秀樹
  • Organizer: 第90回日本生理学会
  • Place of Presentation: タワーホール船堀 ・東京
  • Year and Date: 2013-03-27
• [Presentation] Induction of oligodendrocyte progenitors from mouse iPS cells for transplantation to neonatal white matter injury (2013)
  • Author(s): Sachiyo Misumi
  • Organizer: 日本生理学会
  • Place of Presentation: タワーホール船堀 （東京都）
• [Presentation] iPS 細胞からのオリゴデンドロサイト前駆細胞誘導と未熟ラット脳への移植 (2012)
  • Author(s): 三角吉代、上田佳朋、飛田秀樹ら
  • Organizer: 北米神経科学学科
  • Place of Presentation: ニューオリンズ・ アメリカ合衆国
  • Year and Date: 2012-10-15
• [Presentation] Induction to oligodendrocyte progentors from mouse iPS cells and graft into the immature brain (2012)
  • Author(s): Sachiyo Misumi
  • Organizer: Society for Neuroscience
  • Place of Presentation: Morial Convention Center （米国）
• [Presentation] マウスiPS細胞からｵﾘｺﾞﾃﾞﾝﾄﾞﾛｻｲﾄ前駆細胞への分化誘導と細胞障害抵抗性の検討 (2012)
  • Author(s): Sachiyo Misumi
  • Organizer: 中部生理学会
  • Place of Presentation: 自然科学研究機構 岡崎 カンファレンスセンター （愛知県）
• [Presentation] oligodendrocyte differentiation from mouse pluripotent stem cells (2011)
  • Author(s): Sachiyo Misumi, Ueda Yoshitomo, Masuda Tadashi, Fujita Masataka, Aoyama Mineyoshi, Asai Kiyofumi, Sawamoto Kazunobu, Hida Hideki
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜パシフィコ(神奈川県）
• [Presentation] Oligodendrocyte progenitor cells from mouse induced pluripotent stem cells: selection of A2B5 positive cells and transplantation into the brain (2011)
  • Author(s): Sachiyo Misumi, Yoshitomo Ueda, Masataka Fujita, Tadashi Masuda, Hideki Hida
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington convention center(アメリカ）