Creation and analysis of amygdala-specific gene knockout mice utilizing a dominant-negative mutant of Cre
| Project/Area Number |
23700488
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIYAMA Yuji 東京大学, 医科学研究所, 助教 (90456195)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Cre recombinase / Amygdala / dominant-negative / hyperactive / dominant negative / 部位特異的遺伝子欠損 / PhiC31o / 扁桃体 / ドミナント・ネガティブ / 情動 |
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| Research Abstract |
The purpose of this research is to develop a method to create mice in which the deletion of any gene of interest is highly restricted to the amygdala that subserves emotional functions. For this purpose, we tried to utilize site-specific DNA recombinase PhiC31O and a dominant-negative mutant of Cre to establish highly regional specificity. We determined that phiC31O was effective in mammalian central nervous system in conjunction with the Cre recombinase. And we created and analyzed the transgenic mice expressing Y324F mutant of Cre recombinase to verify its dominant-negative function.
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Report
(4 results)
Research Products
(13 results)
