Creation and analysis of amygdala-specific gene knockout mice utilizing a dominant-negative mutant of Cre

• Project/Area Number: 23700488
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Fusional basic brain science
• Research Institution: The University of Tokyo
• Principal Investigator: KIYAMA Yuji 東京大学, 医科学研究所, 助教 (90456195)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Cre recombinase / Amygdala / dominant-negative / hyperactive / dominant negative / 部位特異的遺伝子欠損 / PhiC31o / 扁桃体 / ドミナント・ネガティブ / 情動

Research Abstract

The purpose of this research is to develop a method to create mice in which the deletion of any gene of interest is highly restricted to the amygdala that subserves emotional functions. For this purpose, we tried to utilize site-specific DNA recombinase PhiC31O and a dominant-negative mutant of Cre to establish highly regional specificity. We determined that phiC31O was effective in mammalian central nervous system in conjunction with the Cre recombinase. And we created and analyzed the transgenic mice expressing Y324F mutant of Cre recombinase to verify its dominant-negative function.

Research Products

• [Journal Article] Lemur kinase 3 deficiency causes pronounced locomotor hyperactivity and impairs endocytic trafficking (2014)
  • Author(s): Inoue T, Hoshina N, Nakazawa T, Kiyama Y, Kobayashi S, Abe T, Yamamoto T, Manabe T and Yamamoto T
  • Journal Title: J. Neurosci. Volume: 34 Pages: 5927-5937
• [Journal Article] LMTK3 deficiency causes pronounced locomotor hyperactivity and impairs endocytic trafficking (2014)
  • Author(s): Takeshi Inoue, Naosuke Hoshina, Takanobu Nakazawa, Yuji Kiyama, Shizuka Kobayashi, Takaya Abe, Toshifumi Yamamoto, Toshiya Manabe, and Tadashi Yamamoto
  • Journal Title: Journal of Neuroscience Volume: 未定
  • Peer Reviewed
• [Journal Article] NMDAR2B tyrosine phosphorylation is involved in thermal nociception (2012)
  • Author(s): Delawary M., Tezuka T., Kiyama Y., Yokoyama K., Wada E., Wada K., Manabe T., Yamamoto T., Nakazawa T.
  • Journal Title: Neurosci. Lett. Volume: 516(2) Pages: 270-273
• [Journal Article] NMDAR2B tyrosine phosphorylation is involved in thermal nociception (2012)
  • Author(s): Delawary M, Tezuka T, Yamamoto T. (9名)
  • Journal Title: Neurosci Lett Volume: Vol.516 Issue: 2 Pages: 270-273
  • DOI: 10.1016/j.neulet.2012.04.007
  • Peer Reviewed
• [Journal Article] Age-dependent regulation of depression-like behaviors through modulation of adrenergic receptor α1A subtype expression revealed by the analysis of interleukin-1 receptor antagonist knockout mice (2011)
  • Author(s): Wakabayashi C., Kiyama Y., Kunugi H., Manabe T., Iwakura Y.
  • Journal Title: Neuroscience Volume: 192 Pages: 475-484
• [Journal Article] Age-dependent regulation of depression-like behaviors through modulation of adrenergic receptor α1A subtype expression revealed by the analysis of interleukin-1 receptor antagonist knockout mice (2011)
  • Author(s): C.Wakabayashi
  • Journal Title: Neuroscience Volume: 192 Pages: 475
  • DOI: 10.1016/j.neuroscience.2011.06.031
  • Peer Reviewed
• [Presentation] The adhesion molecule cadherin 11 is essential for acquiring normal hearing ability (2013)
  • Author(s): 城山優治
  • Organizer: 東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学伊藤国際学術研究センター
• [Presentation] 細胞接着因子Cadherin11は正常な聴覚機能獲得に必須である (2012)
  • Author(s): 城山優治、吉川弥生、竹市雅俊、山岨達也、真鍋俊也
  • Organizer: 第35回日本分子生物学会退会
  • Place of Presentation: マリンメッセ福岡(福岡市)
• [Presentation] 細胞接着分子cadherin 11は正常な聴覚機能獲得に必須である (2012)
  • Author(s): 城山優治 吉川弥生 竹市雅俊 山岨達也 真鍋俊也
  • Organizer: 第３５回日本分子生物学会大会
  • Place of Presentation: マリンメッセ福岡（福岡）
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/NeuronalNetwork/Neuronal_Network/publications.htm
• [Remarks] 東京大学医科学研究所 基礎医科学部門 神経ネットワーク分野
  • URL: http://www.ims.u-tokyo.ac.jp/NeuronalNetwork/Neuronal_Network/Index.html
• [Remarks] 東京大学医科学研究所
  • URL: http://www.ims.u-tokyo.ac.jp/imsut/jp/
• [Remarks] 東京大学医科学研究所 基礎医科学部門 神経ネットワーク分野
  • URL: http://www.ims.u-tokyo.ac.jp/NeuronalNetwork/Neuronal_Network/Index.html