Elucidation of memory mechanisms in primate brain: A combinatorial study using viral vector-based optogenetics and high-field functional MRI
| Project/Area Number |
23700489
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOHEI Ohashi 東京大学, 医学(系)研究科(研究院), 特任講師 (20597629)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 光遺伝学 / オプトジェネティクス / 霊長類 / 大脳皮質 / 視床 / 高磁場fMRI / アデノ随伴ウイルスベクター / レンチウイルスベクター / 記憶 |
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| Research Abstract |
The aim of the present study was to elucidate the role of the neurons in the macaque inferior temporal cortex during the encoding and retrieval of memories by using optogenetic control of the neuronal activities. During the research period, I accomplished the following four works. 1. optogenetic control of cerebellar Purkinje cells in vivo and elucidation of causal relationships between cerebellar Purkinje cells in the lobule VI and blood pressure, 2. development of a novel glass-coated optrode for monkey optogenetics, 3. functional analysis of seven promoters in rat and monkey motor cortex after lentiviral vector-mediated gene transfer, 4. application of optogenetic techniques to rat and monkey thalamus and optimization of experimental conditions of an opt-fMRI.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Selective visualization of rodent and macaque neural circuits using transsynaptic and stationary markers driven by a 2A-based bicistronic lentiviral vector2011
Author(s)
Ohashi, Y., Tsubota, T., Sato, A., Koyano, K.W., Tamura, K., Matsuyama, M., Yaguchi, M., Wang, N., Miyashita, Y.
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Journal Title
Neuroscience Research
Volume: 71supplement
Pages: e411-e411
DOI
Related Report
Peer Reviewed
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[Journal Article] A new glass-coated tungsten optrode enclosing multiple optic fibers for fluorescence measurement, optogenetic photo-stimulation, and single-unit recording in deep brain regions2011
Author(s)
Keita Tamura, Yohei Ohashi, Tadashi Tsubota, Daigo Takeuchi, Toshiyuki Hirabayashi, Masae Yaguchi, Ningqun Wang, Yasushi Miyashita
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Journal Title
Neuroscience Research
Volume: Vol. 71, Suppl.
Pages: e310-e311
DOI
Related Report
Peer Reviewed
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