| Project/Area Number |
23700511
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Ritsumeikan University (2012)
Tottori University (2011) |
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Principal Investigator |
HIGAKI Shogo 立命館大学, 研究機構立命館グローバル・イノベーシ ョン, ポストドクトラルフェロー (70595256)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 魚類 / 始原生殖細胞 / 低温保存 / ガラス化 / 実験動物 / ゼブラフィッシュ / ネッタイツメガエル / 始原生殖細部 |
|---|
| Research Abstract |
Through this study, suitable vitrification solution for cryopreserving zebrafish primordial germ cells was developed. Differentiation ability of the primordial germ cells cryopreserved with the established solution was confirmed. In the absence of other promising cryopreservation methods for zebrafish embryos and oocytes, the established cryopreservation method of primordial germ cells can be applicable for cryobanking and restoration of genetic resources of zebrafish.
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