| Project/Area Number |
23700514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
|
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | トランスジェニックマウス / 顕微注入法 / Cre-loxP / PITT / コンディショナル発現 / コンディショナル / 部位特異的遺伝子挿入 / 顕微注入 |
|---|
| Research Abstract |
The aim of this study is to make our targeted transgenesis method (PITT) more versatile by developing a conditional and tissue-specific expression system applicable to it. For this purpose, we conducted: 1) generation of PITT mice exhibiting tissue-specific transgene expression, 2) development of Dre reporter mouse useful for evaluation of Dre expression, 3) development of new seed mouse line that allows to integrate floxed allele, and 4) development of inducible expression system using KRAB repressor.
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