| Project/Area Number |
23700524
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
MAEDA Eijiro 北海道大学, 大学院・工学研究院, 助教 (20581614)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | バイオメカニクス / メカノバイオロジー / 腱・腱細胞 / カルシウム応答 / メカノトランスダクション / 細胞間情報伝達 / 物質輸送 / MEMS / 腱・靭帯 / 力学刺激 |
|---|
| Research Abstract |
A cell culture device mimicking in vivo environment of tenocytes hasbeen developed, using MEMS fabrication techniques and PDMS. It was demonstratedusing the device that intracellular calcium ion concentration was elevated in response tophysiological mechanical stimulation(4%tensile strain or O.1 mPa fluid shear stress), inparticular that a significantly greater number of tenocytes increased the ion level whensubjected to a combined stimulation of such tensile strain and fluid shear stress, comparedto a control level. In addition, a quantitative evaluation of intercellular gap communicationwas succeeded using the current experimental model using a FLIP protocol.
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