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The gene expression analysis in kidney-like structure compared to Embryonic kidney and adult kidney

Research Project

Project/Area Number 23700537
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionOkayama University

Principal Investigator

KITAMURA Shinji  岡山大学, 岡山大学病院, 助教 (70467752)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords腎臓 / 再生 / 臓器構築 / 遺伝子発現 / 臓器再生 / 幹細胞
Research Abstract

We have succeeded to reconstitute the renal structure from renal stem/ progenitor cells in Vitro. However, it is unknown whether the gene expression in kidney-like structure from renal stem/progenitor cells, in embryonic kidney and in adult kidney is close or not. Here we performed to elucidate the concordance rate between the gene expression in kidney-like structure and the gene expression in embryonic kidney and adult kidney by DNA analysis. The gene expression of kidney-like structure was very close to the embryonic kidney, and was on the time course of kidney development. However, it should be analyze more detail.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (15 results) (of which Invited: 1 results) Book (2 results) Remarks (2 results)

  • [Journal Article] 腹膜透析患者への腹膜機能重症化防止を目指した腹膜再生療法2013

    • Author(s)
      喜多村真治、辻憲二、堀元直哉、杉山斉、槇野博史
    • Journal Title

      the journal of Japanese Society for Dialysis Therapy

      Volume: Vol.46, (2) Pages: 163-5

    • NAID

      10031161745

    • Related Report
      2012 Final Research Report
  • [Journal Article] Right hypoplastic kidney2012

    • Author(s)
      Ayu Ogawa, Shinji Kitamura,Kazunori Nakayama, Hitoshi Sugiyama, Naoya Morisada
    • Journal Title

      Kazumoto Iijima and Hirofumi Makino. Kidney In

      Volume: 82(9) Pages: 1037-1037

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The frequency of Fabry disease with the E66Q variant in the α-galactosidase A gene in Japanese dialysis patients: a case report and a literature review2012

    • Author(s)
      Kikumoto Y, Sugiyama H, Morinaga H, Inoue T, Takiue K, Kitagawa M, Saito D, Takatori Y, Kinomura M, Kitamura S, Akagi S, Sada K, Nakao K, Maeshima Y, Kitayama H, Makino H
    • Journal Title

      Clin Nephrol

      Volume: 78(3) Pages: 224-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A case of focal segmental glomerulosclerosis in an adult patient with hypogammaglobulinemia superimposed on membranoproliferative glomerulonephritis in childhood2012

    • Author(s)
      Tsuji K, Uchida HA, Ono T, Inoue T, Shinagawa K, Kitamura S, Maeshima Y, Sugiyama H, Makino H
    • Journal Title

      BMC Nephrol

      Volume: 13(1) Pages: 46-46

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of adiponectin by aberrantly glycosylated IgA1 in glomerular mesangial cells in vitro and in vivo.2012

    • Author(s)
      Inoue T, Sugiyama H, Kitagawa M, Takiue K, Morinaga H, Ogawa A, Kikumoto Y, Kitamura S, Maeshima Y, Makino H
    • Journal Title

      PLoS One

      Volume: 7(3)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis2012

    • Author(s)
      Takiue K, Sugiyama H, Inoue T, Morinaga H, Kikumoto Y, Kitagawa M, Kitamura S, Maeshima Y, Wang DH, Masuoka N, Ogino K, Makino H
    • Journal Title

      BMC Nephrol

      Volume: 13(1) Pages: 14-14

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of adiponectin by aberrantly glycosylated IgA1 in glomerular mesangial cells in vitro and in vivo.2012

    • Author(s)
      Inoue T, Sugiyama H, Kitagawa M, Takiue K, Morinaga H, Ogawa A, Kikumoto Y, Kitamura S, Maeshima Y, Makino H
    • Journal Title

      PLoS One.

      Volume: 7(3) Issue: 3 Pages: e33965-e33965

    • DOI

      10.1371/journal.pone.0033965

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis.2012

    • Author(s)
      Takiue K, Sugiyama H, Inoue T, Morinaga H, Kikumoto Y, Kitagawa M, Kitamura S, Maeshima Y, Wang DH, Masuoka N, Ogino K, Makino H.
    • Journal Title

      BMC Nephrol.

      Volume: 13(1) Issue: 1 Pages: 14-14

    • DOI

      10.1186/1471-2369-13-14

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] High glucose increases metallothionein expression in renal proximal tubular epithelial cells2011

    • Author(s)
      Ogawa, D., et al
    • Journal Title

      Exp.Diabetes Res.

      Volume: 2011 Pages: 534872-534872

    • DOI

      10.1155/2011/534872

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 腎臓幹/前駆細胞からの In Vitro における三次元的な腎臓構造再構築2012

    • Author(s)
      喜多村真治
    • Organizer
      第三回分子腎フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2012-09-01
    • Related Report
      2012 Final Research Report
  • [Presentation] a cluster of adult kidney stem/progenitor cells reconstitute 3-dimensional nephron structures in vitro2012

    • Author(s)
      喜多村真治
    • Organizer
      第11回 日本再生医療学会学術集会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2012-06-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 成体腎臓幹/ 前駆細胞を使用した腎臓再構築の機能解析:尿細管機能を中心に2012

    • Author(s)
      喜多村真治
    • Organizer
      日本再生医療学会学術集会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2012-06-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 成体腎臓幹/前駆細胞からの腎臓構造再構築とその解析2012

    • Author(s)
      喜多村真治
    • Organizer
      日本動物実験代替法学会
    • Place of Presentation
      慶應義塾大学薬学部 芝共立キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] 成体腎臓幹/前駆細胞を使用した腎臓再構築の機能解析:尿細管機能を中心に2012

    • Author(s)
      喜多村真治、辻憲二、槇野博史
    • Organizer
      第55回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 免疫抑制剤による腎臓の発生・再生過程に及ぼす検討2011

    • Author(s)
      堀元直哉、喜多村真治、辻憲二、槇野博史
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-06-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 包括的な腎臓領域再生治療~臨床応用に向けて2011

    • Author(s)
      喜多村真治
    • Organizer
      日本医工学治療学会学術集会
    • Place of Presentation
      岡山コンベンションセンター、岡山市
    • Year and Date
      2011-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 腹膜透析を含めた腹膜再生療法の開発2011

    • Author(s)
      喜多村真治、杉山斉、槇野博史
    • Organizer
      日本医工学治療学会
    • Place of Presentation
      岡山コンベンションセンター、岡山市
    • Year and Date
      2011-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト腹膜中皮細胞を使用した腹膜再生療法の開発2011

    • Author(s)
      喜多村真治、杉山斉、槇野博史
    • Organizer
      日本透析医学会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Ifosmaide投与後に慢性経過で腎障害をきたした3例2011

    • Author(s)
      氏家はる代、喜多村真治、宮本聡、北川正史、前島洋平、杉山斉、槇野博史
    • Organizer
      日本腎臓学会西部学術集会
    • Place of Presentation
      徳島
    • Related Report
      2011 Research-status Report
  • [Presentation] 免疫抑制剤による腎臓の発生・再生過程に及ぼす検討2011

    • Author(s)
      堀元直哉、喜多村真治、辻憲二、槇野博史
    • Organizer
      日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] A cluster of adult kidney stem/progenitor cells reconstitutes the 3-dimensional nephron structure in vitro.

    • Author(s)
      喜多村真治
    • Organizer
      日本再生医療学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 成体腎臓幹/ 前駆細胞を使用した腎臓再構築の機能解析:尿細管機能を中心に

    • Author(s)
      喜多村真治
    • Organizer
      日本再生医療学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 腎臓幹/前駆細胞からのIn Vitroにおける三次元的な腎臓構造再構築

    • Author(s)
      喜多村真治
    • Organizer
      第三回分子腎フォーラム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 成体腎臓幹/前駆細胞からの腎臓構造再構築とその解析

    • Author(s)
      喜多村真治
    • Organizer
      日本動物実験代替法学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Book] 先天代謝異常症候群(第2 版)下-病因・病態研究、診断・治療の進歩-XIV 結合組織異常 アルポート症候群、別冊先天代謝異常症候群(下)2012

    • Author(s)
      大橋俊孝、喜多村真治
    • Publisher
      日本臨床社
    • Related Report
      2012 Final Research Report
  • [Book] 端医療に関する医療ニーズ/製品開発戦略と臨床で使わなくなる(であろう)薬剤・製品 予測、分担:腎臓領域における再生医療実用化の手ごたえと使わなくなるであろう薬剤・機器・治療法2012

    • Author(s)
      喜多村真治
    • Publisher
      技術情報協会誌
    • Related Report
      2012 Final Research Report
  • [Remarks] 腎臓の機能評価の基礎及び臨床 喜多村 真治日本たばこ産業株式会社 医薬総合研究所 安全性研究所 秦野、神奈川県、平成 24 年 11 月 21 日

    • Related Report
      2012 Final Research Report
  • [Remarks] 腎疾患再生医療の方向性喜多村真治鳥取大学染色工学センター、平成 23 年 4月15日

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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