Protein carrier nanomachine
Project/Area Number |
23700539
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Kyushu University |
Principal Investigator |
MORI Takeshi 九州大学, 工学(系)研究科(研究院), 准教授 (70335785)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | タンパク質 / ナノメディスン / 細胞膜 / エンドサイトーシス / 免疫治療 / がん免疫治療 / デリバリー / レセプター / ドラッグデリバリー / 合成高分子 / 高分子電解質 |
Research Abstract |
Here, we synthesized dextrans modified with trivalent cationic or anionic groups. Aqueous solutions of the cationic and anionic dextrans were then mixed resulting in the formation of polyion complex nanogels (PIC-NGs), which have physically crosslinked salt bridges formed between the cationic and anionic groups. We have designed biotinylated polymers as synthetic receptors that have multiple alkyl groups for endocytotic delivery of target proteins. The polymers were stably attached to a cell surface via multivalent anchoring. The presented biotin was bound to streptavidin (SA) on the cell surface, and, via an endocytotic pathway, the cell rapidly internalized the biotinylated polymer/SA complex.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] A polyion complex nanogel2013
Author(s)
Masafumi Takeo, Takeshi Mori, Takuro Niidome, Shinichi Sawada, Kazunari Akiyoshi, Yoshiki Katayama
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Journal Title
Journal of Colloid and Interface Science
Volume: 390
Issue: 1
Pages: 78-84
DOI
Related Report
Peer Reviewed
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