Development of intracellular glycated products monitoring systems for cellular functional regulation.

• Project/Area Number: 23700545
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo University of Technology
• Principal Investigator: MIKAMI Akane (SAKAGUCHI Akane) 東京工科大学, 応用生物学部, 助教 (70469782)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生体情報 / 計測 / 細胞機能制御 / 細胞内計測 / 糖尿病 / 糖化物 / 転写調節因子 / 生体情報・計測

Research Abstract

Novel transcriptional regulators for intracellular glycated products monitoring and transcriptional regulation systems in mammalian cells have been investigated. A putative glycated product-responsive regulator (GprR) was cloned and found to function as GprR. A regulator based on the GprR was then designed as novel transcriptional regulator which allows intracellular GPs monitoring in mammalian cells.

Research Products

• [Journal Article] Identification and functional analysis of fructosyl amino acid-binding protein from Gram-positive bacterium Arthrobacter sp (2013)
  • Author(s): Sakaguchi-Mikami A, Ferri S, Katayama S, Tsugawa W, Sode K
  • Journal Title: J Appl Microbiol Volume: 114(5) Issue: 5 Pages: 1449-56
  • DOI: 10.1111/jam.12152
  • Peer Reviewed
• [Journal Article] Cloning and Characterization of Fructosamine-6-Kinase from Arthrobacter aurescens (2013)
  • Author(s): Sakaguchi-Mikami A, Kameya M, Ferri S,Tsugawa W, Sode K
  • Journal Title: Appl BiochemBiotechnol Volume: 170 Issue: 3 Pages: 710-717
  • DOI: 10.1007/s12010-013-0229-8
  • Peer Reviewed
• [Journal Article] Construction of a novel glucose-sensing molecule based on a substrate-binding protein for intracellularsensing (2011)
  • Author(s): Sakaguchi-Mikami A, Yamazaki T, Tanigchi A, Sode K, Karube I.
  • Journal Title: Biotechnology and Bioengineering Volume: 108 Issue: 4 Pages: 725-33
  • DOI: 10.1002/bit.23006
  • Peer Reviewed
• [Presentation] 細胞内糖化物応答性転写調節因子の検索 (2013)
  • Author(s): 三上あかね、大矢修一、山崎智彦、軽部征夫
  • Organizer: 第93日本化学会春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス、滋賀
• [Presentation] 細胞内糖化物応答性転写調節因子の検索 (2013)
  • Author(s): 三上あかね・大矢修一・山崎智彦・軽部征夫
  • Organizer: 第 93 日本化学会春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス、滋賀
• [Presentation] 細胞内糖化物測定用転写調節因子の設計 (2012)
  • Author(s): 三上あかね、工藤芳、綾野晃一、山崎智彦、軽部征夫
  • Organizer: 第92回日本化学会春季年会
  • Place of Presentation: 慶応大学矢上・日吉キャンパス(神奈川)
  • Year and Date: 2012-03-25
• [Presentation] 新規糖化物応答性転写調節因子様タンパク質SocRのクローニング及び特性検討 (2012)
  • Author(s): 大矢修一、軽部征夫、三上あかね
  • Organizer: 第22回日本メイラード学会年会
  • Place of Presentation: 東京農工大学、東京
• [Presentation] 糖化タンパク質計測用新規分子認識素子の探索と計測 (2012)
  • Author(s): 三上あかね.
  • Organizer: 第22回日本メイラード学会年会
  • Place of Presentation: 東京農工大学、東京
• [Presentation] 細胞内糖化物測定用転写調節因子の設計 (2012)
  • Author(s): 三上 あかね、工藤芳、綾野晃一、山崎智彦、軽部征夫
  • Organizer: 第92回日本化学会春季年会
  • Place of Presentation: 慶応大学 矢上・日吉キャンパス (神奈川)
• [Presentation] Intracellular sensing and gene regulation using engineered transcriptional regulators (2011)
  • Author(s): T.Yamazaki, A. Mikami, K. Sode, A. Taniguchi, N. Hanagata
  • Organizer: Japan-China International Forum ofAdvanced Research on Biotechnology
  • Place of Presentation: 東京海洋大学品川キャンパス(東京)
  • Year and Date: 2011-11-10
• [Presentation] Engineered transcriptional regulators (2011)
  • Author(s): T.Yamazaki, A. Mikami, K. Sode , A. Taniguchi, N. Hanagata
  • Organizer: Japan-China International Forum of Advanced Research on Biotechnology
  • Place of Presentation: 東京海洋大学 品川キャンパス(東京)
• [Book] バイオセンサーのはなし (2012)
  • Author(s): 軽部征夫
  • Publisher: 日刊工業新聞社
• [Book] 『バイオセンサーのはなし』第3章11節’センサー細胞を用いた毒性評価’ (2012)
  • Author(s): 軽部征夫、編著 (３章１１節 執筆担当 三上あかね)
  • Publisher: 日刊工業新聞社