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Analysis of reverse transmigration of monocytes across endothelium as the anti-atherogenic system.

Research Project

Project/Area Number 23700546
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionKawasaki Medical School

Principal Investigator

HASHIMOTO Ken  川崎医科大学, 医学部, 講師 (80341080)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords動脈硬化 / 血管内皮細胞 / 単球 / 逆浸潤 / TRPV2 / 内皮細胞 / 浸潤 / 血管内皮
Research Abstract

Pathophysiological significance of momocyte reverse transmigration from subendothelium back into the blood stream during atherogenesis is unclear. We established the quantitative evaluation system of reverse transmigration, and revealed that high dose of IL-1β selectively impaired the process. This suggests that in strong inflammatory conditions like atherosclerosis, the clearance activity of pathogenic substances in subendothelium is impaired due to suppressed reverse transmigration. Meanwhile, mechanical stimulations like membrane stretch are generated at the border of migrating monocytes and endothelial cells. We evaluated the role of TRPV2, previously reported mechano-sensitive cation channels, and demonstrated its necessity for cells to move.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (8 results)

  • [Journal Article] Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic β -Adrenergic Stimulation.2013

    • Author(s)
      Sumita Yoshikawa W, Nakamura K, Miura D, Shimizu J, Hashimoto K, Kataoka N, Toyota H, Okuyama H, Miyoshi T, Morita H, Fukushima Kusano K, Matsuo T, Takaki M, Kajiya F, Yagi N, Ohe T, Ito H.
    • Journal Title

      Circ J

      Volume: 77(3) Pages: 741-8

    • NAID

      10031138966

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic β-Adrenergic Stimulation2013

    • Author(s)
      Sumita Yoshikawa W, Nakamura K, Miura D, Shimizu J, Hashimoto K, Kataoka N, Toyota H, Okuyama H, Miyoshi T, Morita H, Fukushima Kusano K, Matsuo T, Takaki M, Kajiya F, Yagi N, Ohe T, Ito H.
    • Journal Title

      Circulation Journal

      Volume: 77 Issue: 3 Pages: 741-748

    • DOI

      10.1253/circj.CJ-12-0779

    • NAID

      10031138966

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium influx through the TRPV1 channel of endothelial cells (ECs) correlates with a stronger adhesion between monocytes and ECs.2012

    • Author(s)
      Himi N
    • Journal Title

      Adv Med Sci.

      Volume: 57 Issue: 2 Pages: 224-229

    • DOI

      10.2478/v10039-012-0044-4

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic β-Adrenergic Stimulation2012

    • Author(s)
      W.S. Yoshikawa, K. Nakamura, D. Miura, J. Shimizu, K. Hashimoto, N. Kataoka, et al.
    • Journal Title

      Circulation Journal

      Volume: 77 Issue: 1 Pages: 741-748

    • DOI

      10.1007/s12576-011-0181-8

    • NAID

      10031138966

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Live-cell visualization of the trans-cellular mode of monocyte transmigration across the vascular endothelium, and its relationship with endothelial PECAM-1.2011

    • Author(s)
      Hashimoto K, Kataoka N, Nakamura E, Hagihara K, Okamoto T, Kanouchi H, Mohri S, Tsujioka K, Kajiya F.
    • Journal Title

      J Physiol Sci

      Volume: 62(1) Pages: 63-9

    • NAID

      10030351477

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cadherins in potential link between atherosclerosis and cancer2011

    • Author(s)
      Hashimoto K, Kataoka N, Mohri S, Kajiya F
    • Journal Title

      Int J Cardiol

      Volume: 149(3) Pages: 397-397

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cadherins in potential link between atherosclerosis and cancer.2011

    • Author(s)
      Hashimoto K
    • Journal Title

      Int J Cardiol.

      Volume: 149(3) Issue: 3 Pages: 397-397

    • DOI

      10.1016/j.ijcard.2011.03.022

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Monocyte trans-endothelial migration augments subsequent transmigratory activity with increased PECAM-1 and decreased VE-cadherin at endothelial junctions.2011

    • Author(s)
      Hashimoto K
    • Journal Title

      Int J Cardiol.

      Volume: 149(2) Issue: 2 Pages: 232-239

    • DOI

      10.1016/j.ijcard.2010.12.018

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Monocyte trans-endothelial migration augments subsequent transmigratory activity with increased PECAM-1 and decreased VE-cadherin at endothelial junctions.2010

    • Author(s)
      Hashimoto K, Kataoka N, Nakamura E, Hagihara K, Hatano M, Okamoto T, Kanouchi H, Minatogawa Y, Mohri S, Tsujioka K, Kajiya F.
    • Journal Title

      Int J Cardiol

      Volume: 149(2) Pages: 232-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 発生段階の心筋細胞の分化・分裂機構の解明に向 けた予備的解析(Preliminary analysis of differentiation and proliferation program of cardiomyocytes in developmental stages.)2013

    • Author(s)
      橋本謙、氏原嘉洋、毛利聡
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Preliminary analysis of differentiation and proliferation program of cardiomyocytes in developmental stages.2013

    • Author(s)
      Hashimoto K
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Real-time observation of actin dynamics in migrating monocytic cells with Lifeact-GFP fusion protein2012

    • Author(s)
      Noriyuki Kataoka, Ken Hashimoto, Takeaki Okamoto, Satoshi Mohri, Fumihiko Kajiya
    • Organizer
      Biomedical Engineering Society AnnualMeeting
    • Place of Presentation
      アトランタ
    • Related Report
      2012 Final Research Report
  • [Presentation] 動脈硬化初期の単球-血管内皮間相互作用におけるメカノセンサー(TRPV2)の役割2012

    • Author(s)
      橋本謙、氏原嘉洋、片野坂友紀、毛利聡
    • Organizer
      第64会日本生理学会中国四国地方会
    • Place of Presentation
      高知
    • Related Report
      2012 Final Research Report
  • [Presentation] Real-time observation of actin dynamics in migrating monocytic cells with Lifeact-GFP fusion protein.2012

    • Author(s)
      Noriyuki Kataoka
    • Organizer
      Biomedical Engineering Society Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 動脈硬化初期の単球-血管内皮間相互作用におけるメカノセンサー(TRPV2)の役割2012

    • Author(s)
      Hashimoto K
    • Organizer
      第64会日本生理学会中国四国地方会
    • Place of Presentation
      高知
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロピペットを用いたケモカイン投与による白血球の遊走制御 Observation of leukocyte migration induced by chemoattractant injection from micropipette2011

    • Author(s)
      片岡則之、橋本謙、毛利聡、梶谷文彦
    • Organizer
      第50回日本生体医工学会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Observation of leukocyte migration induced by chemoattractant injection from micropipette.2011

    • Author(s)
      片岡 則之
    • Organizer
      第50回日本生体医工学会
    • Place of Presentation
      東京電機大学 神田キャンパス
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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