Investigation of nucleic acid type ligand which can use in vivo
Project/Area Number |
23700552
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Hokkaido University |
Principal Investigator |
HYODO Mamoru 北海道大学, 大学院・薬学研究院, 特任助教 (30548186)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | バイオコンジュゲート材料 / アプタマー / リガンド / DDS / 腫瘍血管内皮細胞 / active targeting / Troponin T / リポソーム / SELEX / 脈管形成阻害 / 内在化 |
Research Abstract |
We have isolated the DNA aptamer named AraHHOOI from 12rounds of selection to tumor endothelial cells and counter selection with normal endothelialcells and tumor parenchymal cells. AraHHOOl aptamer showed 44 nM dissociationconstant to tumor endothelial cells and incorporated into tumor endothelial cells.AraHHOOI aptamer binds to Troponin T protein on tumor endothelial cells and AraHHOOlaptamer bound liposome showed 3.5 times higher uptake efficacy compared to normalliposome.
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Report
(3 results)
Research Products
(17 results)
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[Patent(Industrial Property Rights)] トロポニンTと結合するDNA、これを用いた腫瘍組織における血管新生および/または脈管形成抑制剤ならびにこれを用いた細胞内へ目的物質が取り込まれることを促進する剤2012
Inventor(s)
アラ ナズニン,兵藤宣,畠山浩人,樋田京子,大賀則孝,原島秀吉
Industrial Property Rights Holder
バイオメッドコア,アラ ナズニン,兵藤立,畠山浩人,樋田京子,大賀則孝,原島秀吉
Filing Date
2012-06-21
Related Report
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