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Muscle tissue construction by label-free cell assembly based on the magneto-Archimedes effect

Research Project

Project/Area Number 23700557
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University (2012)
Tokyo University of Agriculture and Technology (2011)

Principal Investigator

AKIYAMA Yoshitake  大阪大学, 大学院・工学研究科, 講師 (80585878)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords組織工学 / 磁気アルキメデス効果 / 細胞アセンブリ / ラベルフリー
Research Abstract

In the present study, a cell assembly device for in-vitro three-dimensional (3D) tissue construction based on the magneto-Archimedes effect was designed and fabricated. So as to utilize the magneto-Archimedes effect, it is necessary to add paramagnetic compound like an MRI contrast agent to the culture medium. We confirmed that its cytotoxicity was low enough. We also aggregated myoblast cells with the developed device and tried construct a 3D muscle tissue.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (22 results)

  • [Journal Article] Evaluation of mechanical stimulation effect on cellular orientation under confluence based on 2D-FFT and principal component analysis2012

    • Author(s)
      Y. Akiyama, T. Hoshino, M. Hashimoto, K. Morishima
    • Journal Title

      Journal of Micro-Nano Mechatronics

      Volume: 7(1-3) Pages: 69-77

    • Related Report
      2012 Final Research Report
  • [Journal Article] Room temperature operable autonomously moving bio-microrobot powered by insect dorsal vessel tissue2012

    • Author(s)
      Y. Akiyama, T. Hoshino, K. Iwabuchi, K. Morishima
    • Journal Title

      PLoS ONE

      Volume: 7(7)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Evaluation of mechanical stimulation effect on cellular orientation under confluence based on 2D-FFT and principal component analysis2012

    • Author(s)
      Y. Akiyama, T. Hoshino, M. Hashimoto, K. Morishima
    • Journal Title

      Journal of Micro-Nano Mechat ronics

      Volume: 7 Issue: 1-3 Pages: 69-77

    • DOI

      10.1007/s12213-012-0045-1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapidly-moving insect muscle-powered microrobot and its chemical acceleration.2012

    • Author(s)
      Y. Akiyama, K. Odaira, K. Sakiyama, T. Hoshino, K. Iwabuchi, K. Horishima
    • Journal Title

      Biomedical Microdevices

      Volume: 14 Issue: 6 Pages: 979-986

    • DOI

      10.1007/s10544-012-9700-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell patteming through inkjet printing of one cell per droplet2012

    • Author(s)
      S. Yamaguchi, A. Ueno, Y. Akiyama, K. Morishima
    • Journal Title

      Biofabrication

      Volume: 4 Issue: 4 Pages: 45005-45005

    • DOI

      10.1088/1758-5082/4/4/045005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 磁気アルキメデス効果を用いた電磁石による3次元細胞凝集体構築に関する検討2013

    • Author(s)
      正 典子
    • Organizer
      ロボティクス・メカトロニクス講演会2013
    • Place of Presentation
      つくば国際会議場(茨城)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 磁気アルキメデス効果を用いた電磁石による細胞凝集法の検討2012

    • Author(s)
      正典子, 森島圭祐,秋山佳丈
    • Organizer
      日本バイオマテリアル学会大会シンポジウム2012
    • Place of Presentation
      仙台国際センター(宮城)
    • Year and Date
      2012-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of 3D cell aggregation system using magneto-Archimedes effect2012

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      International Conference on Biofabrication 2012 (Biofabrication 2012)
    • Place of Presentation
      Manchestor (UK)
    • Year and Date
      2012-10-31
    • Related Report
      2012 Final Research Report
  • [Presentation] 磁気アルキメデス効果によるマイクロ流体チップ内での超高速スフェロイドアレイ形成と回収2012

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Label-free ultrarapid spheroid formation in microfluidic chip using magneto-Archimedes effect2012

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems (MEMS)
    • Place of Presentation
      Paris (France)
    • Year and Date
      2012-02-01
    • Related Report
      2012 Final Research Report
  • [Presentation] マイクロ流体チップ内でのノンラベル高速スフェロイドアレイ形成2012

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第21回インテリジェント材料/システムシンポジウム
    • Place of Presentation
      東京女子医科大学(東京)
    • Year and Date
      2012-01-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 磁気アルキメデス効果によるマイクロ流体チップ内での超高速スフェロイドアレイ形成と回収2012

    • Author(s)
      秋山 佳丈
    • Organizer
      第 64 回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of 3D cell aggregation system using magnetoArchimedes effect2012

    • Author(s)
      Y. Akiyama
    • Organizer
      International Conference on Biofabrication 2012
    • Place of Presentation
      Manchester (UK)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Label-free three-dimensional cell aggregation system using magneto-Archimedes effect2012

    • Author(s)
      Y. Akiyama
    • Organizer
      International Symposium at Frontier Research Base for Global Young Researchers
    • Place of Presentation
      大阪大学(大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 磁気アルキメデス効果を用いた電磁石による細胞凝集法の検討2012

    • Author(s)
      正 典子
    • Organizer
      日本バイオマテリアル学会大会シンポジウム2012
    • Place of Presentation
      仙台国際センター(宮城)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Label-free ultrarapid spheroid formation in microfluidic chip using magneto-Archimedes effect2012

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems (MEMS 2012)
    • Place of Presentation
      Marriott Paris Rive Gauche Hotel (Paris, France)
    • Related Report
      2011 Research-status Report
  • [Presentation] マイクロ流体チップ内でのノンラベル高速スフェロイドアレイ形成2012

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第21回インテリジェント材料/システムシンポジウム
    • Place of Presentation
      東京女子医科大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 磁気アルキメデス効果を用いた超高速ラベルフリー細胞アセンブリ法の有用性の検証2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テレサ(京都)
    • Year and Date
      2011-11-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Spheroid array formation by non-label cell manipulation using magneto-Archimedes effect2011

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      The 23th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2011)
    • Place of Presentation
      Nagoya (Japan)
    • Year and Date
      2011-11-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Hollow cylinder-shaped tissue formed by magneto-Archimedes cellular assembly2011

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      International Conference on Biofabrication 2011(Biofabrication 2011)
    • Place of Presentation
      Toyama (Japan)
    • Year and Date
      2011-10-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 磁気アルキメデス効果を用いた非標識超高速細胞アセンブリ法による生体外 3 次元組織構築2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第63回 日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Year and Date
      2011-09-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 磁気アルキメデス細胞アセンブリ法による多層スフェロイドの形成2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第29回日本ロボット学会学術講演会
    • Place of Presentation
      芝浦工業大学(東京)
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Hollow cylinder-shaped tissue formed by magneto-Archimedes cellular assembly2011

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場(富山)
    • Related Report
      2011 Research-status Report
  • [Presentation] Spheroid array formation by non-label cell manipulation using magneto-Archimedes effect2011

    • Author(s)
      Y. Akiyama, K. Morishima
    • Organizer
      The 23th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2011)
    • Place of Presentation
      名古屋大学 (愛知)
    • Related Report
      2011 Research-status Report
  • [Presentation] 磁気アルキメデス効果を用いた超高速ラベルフリー細胞アセンブリ法の有用性の検証2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テルサ(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 磁気アルキメデス効果を用いた非標識超高速細胞アセンブリ法による生体外 3 次元組織構築2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第63回 日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 磁気アルキメデス細胞アセンブリ法による多層スフェロイドの形成2011

    • Author(s)
      秋山佳丈,森島圭祐
    • Organizer
      第29回日本ロボット学会学術講演会
    • Place of Presentation
      芝浦工業大学 (東京)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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