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Induction of tooth constitution cell derived from ES/iPS cells

Research Project

Project/Area Number 23700562
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionKagoshima University

Principal Investigator

AKASAKA Eri  鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (20597562)

Co-Investigator(Renkei-kenkyūsha) SATO Masahiro  鹿児島大学, 医用ミニブタ先端開発研究センター, 教授 (30287099)
SAITOH Issei  新潟大学, 医歯学総合研究科, 准教授 (90404540)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsES細胞 / iPS細胞 / 歯形成細胞 / in vivtro組織再構成 / in vitro組織再構成
Research Abstract

In order to specifically and noninvasively detect a tooth constitution cell among teratoma cells, a construct (pT-ARIP), which connected the fluorescent gene and the drug resistance gene that were present downstream from the specific gene in the ameloblast, was introduced via a gene introduction system using the piggyback transposon. A teratoma was not formed via the introduction of pT-ARIP into iPS cells. Hence, RT-PCR and immunohistological analyses of the primary cell culture of the teratoma derived from iPS cells was conducted before transgenesis. We confirmed the existence of DSPP and AMELX. As mentioned above, a possibility that the differentiation induction of the cell group concerning tooth formation could be carried out from an iPS cell in vitro was shown.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2014 2013 2012 2011 Other

All Journal Article (27 results) (of which Peer Reviewed: 27 results,  Open Access: 3 results) Presentation (15 results)

  • [Journal Article] Novel cancer vaccination system based on human endo-β-N-Acetyl glucosaminidase gene delivery2014

    • Author(s)
      Watanabe S, Haraguchi S, Nakamura S, Sakurai T, Mugikura S, Kajiwara K, Minoiru K, Sato M
    • Journal Title

      Journal of Glycobiology

      Volume: 3 Issue: 01 Pages: 1-1

    • DOI

      10.4172/2168-958x.1000106

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of theα-1,3-galactosyltransferase gene in porcine embryonic fibroblasts2014

    • Author(s)
      Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      Xenotransplantation

      Volume: 21 FEB( Article first published online) Issue: 3 Pages: 291-300

    • DOI

      10.1111/xen.12089

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PiggyBac-mediated generation of stable transfectants with surface HLA expression from a small number of cells.2013

    • Author(s)
      Miura H., Inoko H., Inoue I., Okada Y., Tanaka M., Sato M. and Ohtsuka M.
    • Journal Title

      Anal. Biochem

      Volume: 437 Issue: 1 Pages: 29-31

    • DOI

      10.1016/j.ab.2013.02.003

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mosaic expression of pluripotency-related proteins oct-3/4 and alkaline phosphatase in human pancreatic carcinoma cell PANC-12013

    • Author(s)
      Sato M, Maeda S, Inada E, Saitoh I, Kubota N
    • Journal Title

      Advanced Studies in Biology

      Volume: 5 Pages: 157-172

    • URL

      http://www.doaj.org/doaj?func=issueTOC&isId=150538&uiLanguage=en

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hela cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study.2013

    • Author(s)
      Sato M, Kubota N, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      Advances in Stem Cells

      Volume: 2013 Pages: 15-15

    • DOI

      10.5171/2013.208514

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression2013

    • Author(s)
      Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      Biology

      Volume: 2 Issue: 1 Pages: 341-355

    • DOI

      10.3390/biology2010341

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of hydrodynamics-based gene transfer of nonviral DNA targeted to murine hepatocytes2013

    • Author(s)
      Nakamura S, Maehara T, Watanabe S, Ishihara M, Sato M
    • Journal Title

      Biomed Res Int

      Volume: 2013(2013) Pages: 9-9

    • DOI

      10.1155/2013/928790

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination2013

    • Author(s)
      Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H
    • Journal Title

      Transgenic Research

      Volume: 22 Issue: 4 Pages: 873-875

    • DOI

      10.1007/s11248-013-9703-x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.2013

    • Author(s)
      Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T and Watanabe S
    • Journal Title

      Biotechnology Journal

      Volume: 8 Issue: 11 Pages: 1355-1361

    • DOI

      10.1002/biot.201300169

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] STO feeder cells are useful for propagation of primarily cultured human deciduous dental pulp cells in view of elimination of contaminated bacteria and promotion of cellular outgrowth.2013

    • Author(s)
      Murakami T, Saitoh I, Inada E, Kurosawa M, Iwase Y, Noguchi H, Terao Y, Yamasaki Y, Hayasaki H, Sato M
    • Journal Title

      Cell Medicine

      Volume: 7 Issue: 1-2 Pages: 75-81

    • DOI

      10.3727/215517913x674234

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial and enzyme technology : an efficient and convenient method for MiniPrep analysis of recombinant plasmids2013

    • Author(s)
      Sato M, Inada E, Saitoh I, Matsumoto Y
    • Journal Title

      Journal of Biomedical Science and Engineering

      Volume: 7 Issue: 03 Pages: 105-107

    • DOI

      10.4236/jbise.2014.73013

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary2012

    • Author(s)
      Sato M, Akasaka E, Saitoh I, Ohtsuka M, Watanabe S
    • Journal Title

      Systems Biology in Reproductive Medicine

      Volume: 58 Issue: 3 Pages: 136-141

    • DOI

      10.3109/19396368.2012.656796

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of an α-1,3-galactosyltransferase, an enzyme that creates xenotransplantation-related α-Gal epitope, in pig preimplantation embryos2012

    • Author(s)
      Chi H
    • Journal Title

      Animal Science Journal

      Volume: 83 Issue: 1 Pages: 88-93

    • DOI

      10.1111/j.1740-0929.2011.00964.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Successful Suppression of Endogenous <i>&alpha;-1,3-Galactosyltransferase</i> Expression by RNA Interference in Pig Embryos Generated <i>In Vitro</i>2012

    • Author(s)
      Chi H
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Issue: 1 Pages: 69-76

    • DOI

      10.1262/jrd.10-165M

    • NAID

      130001170088

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequent in vivo electroporation2012

    • Author(s)
      Sato M
    • Journal Title

      Syst Biol Reprod Med

      Volume: 58 Issue: 5 Pages: 278-287

    • DOI

      10.3109/19396368.2012.688088

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the Optimal Concentration of Several Selective Drugs Useful for Generating Multi-Transgenic Porcine Embryonic Fibroblasts2012

    • Author(s)
      Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S.
    • Journal Title

      Reproduction in Domestic Animals/John Wiley & Sons, Inc.

      Volume: 47 Issue: 5 Pages: 759-765

    • DOI

      10.1111/j.1439-0531.2011.01964.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Cre Knock-in Mouse Line on the Sickle Tail Locus Induces Recombination in the Notochord and Intervertebral Discs.2012

    • Author(s)
      Abe, K. et al.
    • Journal Title

      Genesis

      Volume: 50 Issue: 10 Pages: 758-765

    • DOI

      10.1002/dvg.22035

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] CD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyt2012

    • Author(s)
      Ohnami N, Nakamura A, Miyado M, Sato M, Kawano N, Yoshida K, Harada Y, Takezawa Y, Kanai S, Ono C, Takahashi Y, Kimura K, Shida T, Miyado K, Umezawa A
    • Journal Title

      Biol Open

      Volume: 1(7) Issue: 7 Pages: 640-647

    • DOI

      10.1242/bio.20121420

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates2012

    • Author(s)
      Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      J Biomed Sci Eng.

      Volume: 5 Issue: 07 Pages: 406-408

    • DOI

      10.4236/jbise.2012.57051

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of a set of mouse STO feeder cell lines that confer resistance to several types of selective drugs2012

    • Author(s)
      Saitoh I, Sato M, Iwase Y, Akasaka E, Yamasaki Y, Noguchi H
    • Journal Title

      Cell Medicine

      Volume: 3(1) Issue: 1-3 Pages: 97-102

    • DOI

      10.3727/215517912x639414

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice2012

    • Author(s)
      Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB
    • Journal Title

      Experimental Animals

      Volume: 61 Issue: 5 Pages: 489-502

    • DOI

      10.1538/expanim.61.489

    • NAID

      10031121853

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent transgenic mice suitable for multi-color aggregation chimera studies2012

    • Author(s)
      Ohtsuka M, Miura H, Gurumurthy CB, Kimura M, Inoko H, Yoshimura S, Sato M
    • Journal Title

      Cell and Tissue Research

      Volume: 350 Issue: 2 Pages: 251-260

    • DOI

      10.1007/s00441-012-1470-0

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of limb loss in an experimentally induced hindlimb ischemic model by fibroblast growth factor-2/fragmin/protamine microparticles as a delivery system2012

    • Author(s)
      Nakamura S, Ishihara M, Takikawa M, Kishimoto S, Isoda S, Fujita M, Sato M, Maehara T
    • Journal Title

      Tissue Engineering. Part A

      Volume: 18(21-22) Issue: 21-22 Pages: 2239-2247

    • DOI

      10.1089/ten.tea.2011.0741

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Delivery system for autologous growth factors fabricated with low-molecular-weight heparin and protamine to attenuate ischemic hind-limb loss in a mouse model2012

    • Author(s)
      Nakamura S, Takikawa M, Ishihara M, Nakayama T, Kishimoto S, Isoda S, Ozeki Y, Sato M, Maehara T
    • Journal Title

      Journal of Artificial Organs

      Volume: 15(4) Issue: 4 Pages: 375-385

    • DOI

      10.1007/s10047-012-0658-0

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs2011

    • Author(s)
      Ohtsuka, M., Miura, H., Nakaoka, H., Kimura, M., Sato, M., Inoko, H.
    • Journal Title

      Transgenic Research

      Volume: 21:1 Issue: 1 Pages: 225-226

    • DOI

      10.1007/s11248-011-9505-y

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Whole-genome amplification-based GenomiPhi for multiple genomic analysis of individual early porcine embryos2011

    • Author(s)
      Akasaka E
    • Journal Title

      Theriogenology

      Volume: 75 Issue: 8 Pages: 1543-1549

    • DOI

      10.1016/j.theriogenology.2010.12.018

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Liver lobe-and strain-difference in gene expression after hydrodynamics-based gene delivery in mice

    • Author(s)
      Nakamura S, Maehara T, Ishihara M, Sato M
    • Journal Title

      Animal Biotechnology

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Multipotency of juvenile human buccal epithelial cells2014

    • Author(s)
      Murakami T, Saitoh I, Iwase Y, Inada E, Mastuyama J, Ohshima H, Hayasaki H, Sato M
    • Organizer
      AADR
    • Place of Presentation
      North Carolina
    • Related Report
      2013 Final Research Report
  • [Presentation] gcr2タンパクは糖鎖を介してI型BMP受容体と結合しシグナル伝達制御に関与する2013

    • Author(s)
      渡部聡、梶原景正、麥倉真一郎、桜井敬之、中村伸吾、木村穣、佐藤正宏
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] RISPR/Cas9による遺伝子編集と標的毒素法との組み合わせは、α-1,3-galactosyltransferase遺伝子を完全に KOしたブタ胎仔性線維芽細胞の効率的作製に有効である2013

    • Author(s)
      佐藤正宏、三好和睦、長尾洋三、西洋平、大塚正人、中村伸吾、桜井敬之、渡部聡
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] ハイドロダイナミクスに基づく生体内遺伝子導入における外来遺伝子発現のマウス系統差および肝臓ローブ間の差2013

    • Author(s)
      中村伸吾、前原正明、渡部聡、石原雅之、佐藤正宏
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Syngenic grafting of a whole male juvenile gonadal tissue into the adult testes confers successful spermatogenesis in mice2013

    • Author(s)
      Tada N, Kanai F, Nakamura E, Lu H, Saito M, Sato M
    • Organizer
      The 3rd World Congress of the International Society for Fertility Preservation
    • Place of Presentation
      Malia Valencia Hotel, Valencia, Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013

    • Author(s)
      郡山美優,稲田絵美,齋藤一誠,三浦浩美,大塚正人,中村伸吾,桜井敬之,渡部聡,三好和睦,佐藤正宏
    • Organizer
      第106回日本繁殖生物学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト乳歯歯髄由来iPS細胞樹立におけるフィーダー細胞選択の重要性2013

    • Author(s)
      村上智哉,齊藤一誠,稲田絵美,岩瀬陽子,長谷川大子,窪田直子,松本祐子,大島邦子,岡暁子,山崎要一,早崎治明
    • Organizer
      第51回日本小児歯科学会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] 受精卵へのiCre mRNA注入によるターゲットトランスジェ寝詩ス法(PITT法)の効率改善2013

    • Author(s)
      大塚正人、三浦浩美、佐藤正宏、木村穣
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      茨城
    • Related Report
      2013 Final Research Report
  • [Presentation] Attempts to produce transgenic mice by a novel in vivo method, as an alternative to the pre-existing pronuclear microinjection-based transgenesis2013

    • Author(s)
      Sato M, Akasaka E, Saito I, Ohtsuka M, Watanabe S
    • Organizer
      11th Transgenic Technology Meeting
    • Place of Presentation
      GuangzhouCity,P.R.China,中国
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス成熟卵胞細胞への生体内遺伝子導入法の開発2012

    • Author(s)
      佐藤正宏、赤坂恵理、齋藤一誠、大塚正人、渡部聡
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス肝臓を標的としたplasmidDNAの効率的な遺伝子導入法の開発2012

    • Author(s)
      中村伸吾、前原正明、渡部聡、石原雅之、佐藤正宏
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトEndoβ-N-acetylglucosaminidase遺伝子を用いた新たなCancer vaccination法の開発2012

    • Author(s)
      渡部聡、原口清輝、中村伸吾、桜井敬之、梶原景正、麦倉信一郎、佐藤正宏
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] PITT法を利用して作製したノックダウンマウスにおけるノックダウン効果の比較解析2012

    • Author(s)
      三浦浩美、佐藤正宏、大塚正人
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] ENDOGALC/TARGETED TOXIN-BASED SELECTION SYSTEM AS A USEFUL TOOL FOR ENRICHMENT OF TRANSGENE-HIGH-EXPRESSING MAMMALIAN CELLS2012

    • Author(s)
      Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Organizer
      9th Asian Reproductive Biotechnology Society (ARBS) conference
    • Place of Presentation
      Edsa Shangri-La Hotel, Mandaluyong City, Manila, Phillipine
    • Related Report
      2013 Final Research Report
  • [Presentation] プラスミドDNAの卵管内注入、続く電気穿孔法によるマウス着床前胚への遺伝子導入法の開発2012

    • Author(s)
      佐藤正宏、赤坂恵理、齋藤一誠、大塚正人、渡部聡
    • Organizer
      第59回日本実験動物学会総会
    • Place of Presentation
      大分
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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