Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction
| Project/Area Number |
23700566
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AKIMOTO Aya 東京大学, 大学院・工学系研究科, 助教 (10585805)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | バイオマテリアル / 刺激応答性表面 / 生体分子応答性高分子 / コントロールドリリース / 遺伝子導入 / リバーストランスフェクション |
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| Research Abstract |
In this study, we aimed to improve the efficiency of reverse transfection, one of the economic and low cytotoxic gene introduction methods, by preparing novel stimuli-responsive polycation releasing surface. Polycation release was examined by using cysteine as stimuli. As a result, polycation was released from the surface by applying cysteine containing aqueous solution which was nontoxic to cells. Moreover, 293T cells were well adhered to the surface and surface showed any cytotoxic activities. This functional surface would be potentially useful as experimental platform for gene introduction.
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Report
(3 results)
Research Products
(2 results)
