| Project/Area Number |
23700569
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
ISHIKANE Shin 独立行政法人国立循環器病研究センター, 研究所, 客員研究員 (40470190)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 卵膜 / 間葉系幹細胞 / 細胞シート / パラクライン / 心筋梗塞 / 血管新生効果 / 他家移植 / 前臨床研究 |
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| Research Abstract |
To develop effective treatments for chronic heart failure, we used fetal membrane (FM) derived mesenchymal stem cell (MSC) sheets. In a rat chronic myocardial infarction (MI) model, the allogeneic FM-MSC sheets transplantation had significantly reversed wall thinning in the scar area and had improved cardiac function as well as the autologous bone marrow derived MSC sheets transplantation. However, the allogeneic FM-MSC sheets transplantation did not improve cardiac function in a mini-pig MI model. To improve secretion of angiogenic factors from the FM-MSC sheets, we transfected siRNA-int6 to the FM-MSC. The siRNA-int6 transfection reduced the int6 expression in the FM-MSC, however did not increase the HIF2alpha, HIF1 and VEGF expressions. In a mini-pig acute MI model, angiogenic effect was observed by intra myocardial injection of the siRNA-int6 plasmid-GenomeONE mixture.
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