Development of multi-growth factor secreted fetal membrane-derived mesenchymal stem cell sheets
Project/Area Number |
23700569
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | National Cardiovascular Center Research Institute |
Principal Investigator |
ISHIKANE Shin 独立行政法人国立循環器病研究センター, 研究所, 客員研究員 (40470190)
|
Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 卵膜 / 間葉系幹細胞 / 細胞シート / パラクライン / 心筋梗塞 / 血管新生効果 / 他家移植 / 前臨床研究 |
Research Abstract |
To develop effective treatments for chronic heart failure, we used fetal membrane (FM) derived mesenchymal stem cell (MSC) sheets. In a rat chronic myocardial infarction (MI) model, the allogeneic FM-MSC sheets transplantation had significantly reversed wall thinning in the scar area and had improved cardiac function as well as the autologous bone marrow derived MSC sheets transplantation. However, the allogeneic FM-MSC sheets transplantation did not improve cardiac function in a mini-pig MI model. To improve secretion of angiogenic factors from the FM-MSC sheets, we transfected siRNA-int6 to the FM-MSC. The siRNA-int6 transfection reduced the int6 expression in the FM-MSC, however did not increase the HIF2alpha, HIF1 and VEGF expressions. In a mini-pig acute MI model, angiogenic effect was observed by intra myocardial injection of the siRNA-int6 plasmid-GenomeONE mixture.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Comparison of angiogenic, cytoprotective, and immunosuppressive properties of human amnion- and chorion-derived mesenchymal stem cells.2014
Author(s)
Yamahara K, Harada K, Ohshima M, Ishikane S, Ohnishi S, Tsuda H, Otani K, Taguchi A, Soma T, Ogawa H, Katsuragi S, Yoshimatsu J, Harada-Shiba M, Kangawa K, Ikeda T.
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Journal Title
PLoS One. 2014 Feb 14;9(2):e88319.
Volume: 9
Issue: 2
Pages: e88319-e88319
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Allogeneic transplantation of fetal membrane-derived mesenchymal stem cell sheets increases neovascularization and improves cardiac function after myocardial infarction in rats.2013
Author(s)
Shin Ishikane, Hiroshi Hosoda, Kenichi Yamahara, Yoshiharu Akitake, Jung Kyoungsook, Kenichi Mishima, Katsunori Iwasaki, Michihiro Fujiwara, Mikiya Miyazato, Kenji Kangawa, Tomoaki Ikeda.
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Journal Title
Transplantation
Volume: 96
Issue: 8
Pages: 697-706
DOI
Related Report
Peer Reviewed
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[Journal Article] Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.2012
Author(s)
Ohshima M, Yamahara K, Ishikane S, Harada K, Tsuda H, Otani K, Taguchi A, Miyazato M, Katsuragi S, Yoshimatsu J, Kodama M, Kangawa K, Ikeda T.
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Journal Title
J Mol Cell Cardiol.
Volume: 53(3)
Issue: 3
Pages: 420-428
DOI
Related Report
Peer Reviewed
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