| Project/Area Number |
23700588
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Medical systems
|
|---|
| Research Institution | Himeji Dokkyo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 検査 / 診断システム ユビキチン化 / ユビキチン化 |
|---|
| Research Abstract |
Protein ubiquitination is undertaken through an enzymatic cascade comprising ubiquitin-activating (E1), ubiquitin-conjugating (E2), and ubiquitin-ligating (E3) enzymes. This ubiquitination reaction is related to several diseases such as bladder cancer, prostate cancer, kidney cancer, leukemia. The high sensitive detection of the degree of the ubiquitination leads to the diagnosis of these diseases. In this study, (1) the artificial E3 was designed for the detection of the ubiquitination without a substrate and a tag (2) the micro bioactivity analyzer (AMIS-101) had a high sensitivity for detecting the degree of the ubiquitination of the artificial E3 in the solution including the purified ubiquitin, E1, E2. (3) in human acute myelogenous leukemia cells with treatment of the cancer drugs, the detection system of the ubiquitination of the artificial E3 using AMIS-101 made it possible to the observation of the small amount of the E2 enzyme activity.
|
