Development of molecular-targeted bubbles based on clinically available ultrasound contrast agent

• Project/Area Number: 23700591
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical systems
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: OTANI Kentaro 独立行政法人国立循環器病研究センター, 研究所, 研究員 (50470191)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクロ・ナノデバイス / 生体分子 / 超音波 / 分子イメージング / 薬学

Research Abstract

In the present study, we developed the preparation method of targeted-bubbles for ultrasound molecular imaging based on clinically available ultrasound contrast agent, Sonazoid. By modifying Sonazoid with lactadherin, the number of adhered bubbles to integrin avb3-expresssing endothelial cells was increased significantly. Our results would imply the feasibility of lactadherin-bearing Sonazoid as ultrasound contrast agent for angiogenesis. The future in vivo studies would be of benefit.

Research Products

• [Journal Article] Feasibility of lactadherin-bearing clinically available microbubbles as ultrasound contrast agent for angiogenesis (2013)
  • Author(s): Kentaro Otani, Kenichi Yamahara
  • Journal Title: Mol Imaging Biol Volume: 15(5) Pages: 534-41
  • Peer Reviewed
• [Journal Article] Development of antibody-carrying microbubbles based on clinically available ultrasound contrast agent for targeted molecular imaging : a preliminary chemical study (2011)
  • Author(s): Kentaro Otani, Kenichi Yamahara
  • Journal Title: Mol Imaging Biol Volume: 13(2) Pages: 250-6
  • Peer Reviewed
• [Presentation] Development of integrin avb3-targeted microbubbles based on clinically available ultrasound contrast agent (2014)
  • Author(s): Kentaro Otani
  • Organizer: 19th European Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: Hilton Hotel Rotterdam(ロッテルダム)
• [Presentation] Development of molecular targeted-bubbles based on Sonazoid (2014)
  • Author(s): Kentaro Otani
  • Organizer: The 6th Asian Conference on Ultrasound Contrast Imaging
  • Place of Presentation: パシフィコ横浜 (横浜)
• [Presentation] Development of integrin avb3-targeted microbubbles based on clinically available ultrasound contrat agent (2014)
  • Author(s): Kentaro Otani
  • Organizer: 19th European Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: Hilton Hotel Rotterdam
• [Presentation] Sonazoidを基盤とした分子標的気泡作成法の開発とその特性評価 (2013)
  • Author(s): 大谷健太郎
  • Organizer: 日本超音波医学会第40回関西地方会学術集会
  • Place of Presentation: 大阪国際会議場 (大阪)
• [Presentation] Sonazoidを基盤とした分子標的気泡作成法の開発とその特性評価 (2013)
  • Author(s): 大谷健太郎
  • Organizer: 日本超音波医学会 第40回関西地方会学術集会 シンポジウム２
  • Place of Presentation: 大阪国際会議場
• [Presentation] Feasibility of lactadherinbearing clinically available microbubbles as an ultrasound contrast agent for angiogenesis (2012)
  • Author(s): Kentaro Otani
  • Organizer: 61st Annual Scientific Session of American College of Cardiology
  • Place of Presentation: McComick Place (ロサンゼルス)
• [Presentation] Feasibility of lactadherin-bearing clinically available microbubbles as an ultrasound contrast agent for angiogenesis (2012)
  • Author(s): Kentaro Otani
  • Organizer: American College of Cardiology 61st Annual Scientific Session
  • Place of Presentation: McCormick Place, Chicago
• [Presentation] Sonazoidと生体内分子の混合による簡便な分子標的気泡作成法の開発 (2011)
  • Author(s): 大谷健太郎、池田智明
  • Organizer: 第22回日本心エコー図学会学術集会
  • Place of Presentation: 鹿児島市民文化ホール (鹿児島)
• [Presentation] Sonazoidと生体内分子の混合による簡便な分子標的気泡作成法の開発 (2011)
  • Author(s): 大谷健太郎、池田智明
  • Organizer: 第22回日本心エコー図学会学術集会
  • Place of Presentation: 鹿児島市民文化ホール
• [Presentation] Sonazoidを基盤としたインテグリンavb3標的気泡作成法の開発 (2011)
  • Author(s): 大谷健太郎、池田智明
  • Organizer: 日本超音波医学会第84回学術集会
  • Place of Presentation: グランドプリンスホテル新高輪
• [Book] Feasibility of clinical application of ultrasound molecular imaging. Ultrasound imaging-medical applications. (2011)
  • Author(s): Kentaro Otani
  • Publisher: InTech, Rijeka, Croatia
• [Book] Feasibility of clinical application of nultrasound molecular imaging (2011)
  • Author(s): Kentaro Otani
  • Total Pages: 18
  • Publisher: Intech
• [Remarks] 平成24年度日本心エコー図学会海外学会発表優秀論文賞受賞