The effects of early exercise on brain damage and recovery after focal cerebral infarction in rats.

• Project/Area Number: 23700609
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Kagoshima University
• Principal Investigator: MATSUDA Fumiyo 鹿児島大学, 医学部, 医学部 (70437953)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 実験的脳梗塞ラットモデル / 早期運動療法 / 神経栄養因子 / 理学療法学 / MidKine / 脳梗塞 / 運動療法 / 早期 / Midkine

Research Abstract

The purpose of this study was investigate that the effect of early exercise (this study used the treadmill exercise) after cerebral infarction in the rat. Next day after surgery, stroke rats were randomly assigned to two groups: treadmill-exercised group or non-exercised controls. After ischemia, the rats were received to run on a treadmill for 20 min once a day. All rats were observed the absence of motor coordination and neurological deficits after surgery. Over time motor coordination and neurological deficits improved more in the exercised group than in the non-exercised group. The cellular expression levels of MK were significantly increased in the peri-infarct area of the exercised rats. Therefore The purpose is to confirm the effect of MK gene knockout on cerebral infarction in mouse and the relation of MK with theregeneration. The ratios of infarct volume to cerebral hemisphere were not different between KO and wild type mice at 3 days and 7days. The expression of glial fibrially acidic protein (GFAP) was more and more expressed in periinfarct area after insult. GFAP staining areas in astrocytes (calculated as a ratio of stained areas to hemisphere)were significantly decreased in parietal lobe of KO mice compared with that in wild type mice at 3 days, however, it was not significantly different at 7 days. Since MK has a role as achemotaxis of microglias and macrophages, the Iba1 expression was studied with immunostaining, which showed no significant difference. The results were suggestive that in KO mice the expression of 機関番号：17701 研究種目：若手研究(B) 研究期間：2011 ～ 2012 課題番号：23700609 研究課題名（和文） 脳血管障害後の早期運動療法介入が脳の損傷と回復に与える影響について 研究課題名（英文） The effects of early exercise on brain damage and recovery after focal cerebral infarction in rats. 研究代表者 松田 史代（MATSUDA FUMIYO）鹿児島大学・医学部・助教 研究者番号：70437953 GFAP was delayed compared with in wild type mice. MK participates in the development of the reactive astrocytes after cerebral infarction.

Research Products

• [Journal Article] Potential of edaravone for neuroprotection in neurologic diseases that do not involve cerebral infarction (2011)
  • Author(s): Kikuchi K, Kawahara KI, Uchikado H, Miyagi N, Kuramoto T, Miyagi T, Morimoto Y, Ito T, Tancharoen S, Miura N, Takenouchi K, Oyama Y, Shrestha B, Matsuda F, Yoshida Y, Arimura S, Mera K, Tada KI, Yoshinaga N, Maenosono R, Ohno Y, Hashiguchi T, Maruyama I, Shigemori M
  • Journal Title: Exp Ther Med Volume: 2(5) Pages: 771-775
  • Peer Reviewed
• [Journal Article] 虚血条件下での神経様細胞におけるエダラボンの HMGB1 に対する効果(解説) (2011)
  • Author(s): 菊池清志, 川原幸一, 松田史代, 伊藤隆史, 森元陽子, TancharoenSalunya, 三浦直樹, 竹重暢之, 折戸公彦, 山下伸, 内門久明, 宮城尚久, 塩見直人, 倉本晃一, 徳富孝志, 重森稔, 坂本六大, 菊池千恵美, 飯田成美, 吉田義弘, 橋口照人, 丸山征郎, 森岡基浩, 田中永一郎
  • Journal Title: 神経外傷(0389-5610) Volume: 34巻 2 号 Pages: 225-227
  • Peer Reviewed
• [Presentation] 成長因子ミッドカイン(MK)の欠損がマウス骨格筋損傷病態に与える影響 (2012)
  • Author(s): 生友聖子、榊間春利、松田史代、甲斐千尋、吉田義弘
  • Organizer: 第35回日本神経科学学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-18
• [Presentation] 中大脳動脈閉塞血流再開の有無による梗塞巣周囲の組織学的変化について (2012)
  • Author(s): 福田祐子, 松田史代, 榊間春利, 吉田義弘, 米和徳, 三宅智
  • Organizer: 第47回日本理学療法学術大会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-05-26
• [Presentation] 中大脳動脈閉塞血流再開の有無による梗塞巣周囲の組織学的変化について. (2012)
  • Author(s): 福田祐子
  • Organizer: 第47回日本理学療法学術大会
  • Place of Presentation: 神戸
• [Presentation] 成長因子ミッドカイン（MK）の欠損がマウス骨格筋損傷病態に与える影響 (2012)
  • Author(s): 生友聖子
  • Organizer: 第35回日本神経科学学会
  • Place of Presentation: 名古屋
• [Presentation] 神経栄養因子ミッ ドカイン遺伝子の欠損がマウス実験的脳梗塞の病態に与える影響について (2011)
  • Author(s): 吉田義弘、生友聖子、宮崎晋宏、山崎芳樹、沢田浩暢、木下香菜、園知佐子、用皆正文、榊間春利、松田史代
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-13
• [Presentation] The effects of early exercise on brain damage and recovery after forcal cerebral infarction in rats (2011)
  • Author(s): Fumiyo Matsuda, Harutoshi Sakakima, Kazunori Yone, Yoshihiro Yoshida
  • Organizer: WCPT
  • Place of Presentation: Amsterdam
  • Year and Date: 2011-06-21
• [Presentation] The effects of early low-intensity exercise on rat soleus after focal cerebral infarction in rats (2011)
  • Author(s): Megumi Sakasegawa, Fumiyo Matsuda, Ai Irie, Keiko Ikeda, Harutoshi Sakakima, Kazunori Yone, Yoshihiro Yoshida
  • Organizer: WCPT
  • Place of Presentation: Amsterdam
  • Year and Date: 2011-06-21