The mechanism of the skeletal muscle atrophy; comparing rats and mice, medaka.

• Project/Area Number: 23700856
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Japan Aerospace Exploration Agency
• Principal Investigator: TERADA Masahiro 独立行政法人宇宙航空研究開発機構, 宇宙科学研究所, 研究員 (10553422)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 筋萎縮 / 骨格筋 / 萎縮 / メダカ

Research Abstract

We can simulate the skeletal muscle atrophy by using hindlimb suspension model. It was known that the skeletal muscle was atrophy during space staying. In this experiment, we compare these two models, hindlimb suspension and space flown mice, to investigate the mechanism of the skeletal muscle atrophy. We extracted RNA form these samples. RNA was hybridized to the Whole Mice Genome (4 X 44K) Oligo Microarray (Agilent Technologies) according to the manufacturer's protocol. Slide scanning was performed using the Agilent DNA Microarray Scanner.

Research Products

• [Journal Article] Genetic analysis of the human hair roots as a tool for spaceflight experiments (2013)
  • Author(s): Terada, M., Seki, M., Higashibata, A., Yamada, S., Takahashi, R., Majima, H., Yamazaki, T., Watanabe-Asaka, T., Niihori, M., Mukai, C., Ishioka, N.
  • Journal Title: Advances in Bioscience and Biotechnology Volume: 4 Pages: 75-88
• [Journal Article] Genetic analysis of the human hair roots as a tool for spaceflight experiments. (2013)
  • Author(s): Terada, M., Seki, M., Higashibata, A., Yamada, S., Takahashi, R., Majima, H., Yamazaki, T., Watanabe-Asaka, T., Niihori, M., Mukai, C., Ishioka, N.
  • Journal Title: Advances in Bioscience and Biotechnology Volume: 4 Pages: 75-88
  • Peer Reviewed
• [Journal Article] Effects of mechanical over-loading on the properties of soleus muscle fibers, with or without damage, in wild type and mdx mice. (2012)
  • Author(s): Terada M, Kawano F, Ohira T, Nakai N, Nishimoto N, Ohira Y.
  • Journal Title: PLoS One Volume: 7(4)
  • Peer Reviewed
• [Journal Article] Biomedical analysis of rat body hair after (2012)
  • Author(s): M. Terada, et al.
  • Journal Title: Acta Astronautica Volume: 73 Pages: 23-29
  • URL: http://www.sciencedirect.com/science/article/pii/S0094576511003766
  • Peer Reviewed
• [Presentation] The Effects on Gene Expression on Human Hair and Mice Skin by Spaceflight (poster) (2013)
  • Author(s): Terada, M., Yamada, S., Seki, M., Takahashi, R., Higashibata, A., Majima, H., Ohshima, H., Ohira, Y., Ishioka, N., Mukai, C.
  • Organizer: American Society for Gravitational and Space Biology, 29th Annual Meeting
  • Place of Presentation: Orland, FL, USA
• [Presentation] The Genetic Analyses on Human Hair and Mice Skin (Poster) (2013)
  • Author(s): Terada, M., Yamada, S., Seki, M., Takahashi, R., Higashibata, A., Majima, H., Ohshima, H., Ohira, Y., Ishioka, N., Mukai, C.
  • Organizer: 19th IAA Humans in Space Symposium
  • Place of Presentation: Cologne, Germany
• [Presentation] 宇宙飛行による毛髪での遺伝子変化(ポスター) (2013)
  • Author(s): 寺田昌弘、山田深、関真也、高橋理佳、東端晃、馬嶋秀行、大島博、石岡憲昭、向井千秋
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] The Effects on Gene Expression on Human Hair and Mice Skin by Spaceflight (2013)
  • Author(s): Terada, M., Yamada, S., Seki, M., Takahashi, R., Higashibata, A., Majima, H., Ohshima, H., Ohira, Y., Ishioka, N., Mukai, C.
  • Organizer: American Society for Gravitational and Space Biology, 29th Annual Meeting
  • Place of Presentation: Orland, FL, USA
• [Presentation] The Genetic Analyses on Human Hair and Mice Skin (2013)
  • Author(s): Terada, M., Yamada, S., Seki, M., Takahashi, R., Higashibata, A., Majima, H., Ohshima, H., Ohira, Y., Ishioka, N., Mukai, C.
  • Organizer: 19th IAA Humans in Space Symposium
  • Place of Presentation: Cologne, Germany
• [Presentation] 宇宙飛行による毛髪での遺伝子変化 (2013)
  • Author(s): 寺田昌弘、山田深、関真也、高橋理佳、東端晃、馬嶋秀行、大島博、石岡憲昭、向井千秋
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Genetic and Proteomics Analyses of Space flown Mice Skin (2012)
  • Author(s): Masahiro Terada, Rika Takahashi, Masaya Seki, Akira Higashibata, Hideyuki J. Majima, Yoshinobu Ohira, Chiaki Mukai, Noriaki Ishioka.
  • Organizer: The 33rd Annual International Gravitational Physiology Meeting
  • Place of Presentation: Aberdeen, United Kingdom
• [Presentation] The change of gene expression on the mice skin exposed to various stress environments (2012)
  • Author(s): Masahiro Terada, Rika Takahashi, Masaya Seki, Akira Higashibata, Hideyuki J. Majima, Yoshinobu Ohira, Chiaki Mukai, Noriaki Ishioka.
  • Organizer: 28th Annual Meeting of the American Society for Gravitational and Space Research
  • Place of Presentation: New Orleans, USA
• [Presentation] Genetic Analyses of Long- and Short-Term Space Flown Mice Skin (2012)
  • Author(s): Masahiro Terada, Rika Takahashi, Masaya Seki, Akira Higashibata, Hideyuki J. Majima, Yoshinobu Ohira, Chiaki Mukai, Noriaki Ishioka.
  • Organizer: 第26回日本宇宙生物科学会学術集会
  • Place of Presentation: 徳島、日本