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Development of an electron paramagnetic resonance spin trapping method for analysis of the scavenging capacities of food samples for multiple ROS

Research Project

Project/Area Number 23700897
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits, studies on eating habits
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KAMEYA HIROMI  独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品安全研究領域, 主任研究員 (20585955)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsESR / スピントラップ法 / 酸化評価 / 国際情報交流 ロシア / 電子スピン共鳴分光 / 抗酸化能 / 機能性 / 国際情報交流 ロシア
Research Abstract

We have developed an electron spin resonance (ESR) spin trapping method for analysis of the scavenging capacities of food samples for multiple ROS, utilizing the same photolysis procedure for generating each type of radical. The optimal measuring conditions of hydroxyl, superoxide, alkoxyl, peroxyl, alkyl radical, and singlet oxygen scavenging capacity were determined. Quantification of radical adducts was found to be highly reproducible, with variations of less than 4%. The optimized EPR spin trapping method was used to analyze the scavenging capacities of 54 different vegetable extracts for multiple radicals, and the results were compared with oxygen radical absorption capacity values. Good correlations between the two methods were observed for superoxide and alkoxyl radicals, but not for hydroxyl.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (8 results)

  • [Journal Article] Comparison of scavenging capacities of vegetables by ORAC and EPR2013

    • Author(s)
      Hiromi Kameya, Jun Watanabe, Yuko Takano-Ishikawa, Setsuko Todoriki
    • Journal Title

      Food Chemistry

      Volume: 145 Pages: 866-873

    • DOI

      10.1016/j.foodchem.2013.09.015

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photochemical method to evaluate oxidation levels in heat-treated vegetable oil : comparison with peroxide-value and acid-value2013

    • Author(s)
      Hiromi Kameya, Mitsuko Ukai
    • Journal Title

      Journal of Food Science and Engineering

      Volume: 3 Pages: 299-307

    • URL

      http://www.davidpublishing.org/show.html?13233

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] キノコ熱水抽出物のESRスピントラップ法によるラジカル捕捉活性評価と ORAC法2013

    • Author(s)
      菅野友美、川村翔栄、原田栄津子、亀谷宏美、鵜飼光子、大澤俊彦
    • Journal Title

      日本食品科学工学会誌

      Volume: 60 Pages: 173-178

    • NAID

      10031163930

    • URL

      https://www.jstage.jst.go.jp/article/nskkk/60/4/60_173/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ESRスピントラップ法による緑茶のスーパーオキシドラジカル捕捉活性計測方法2013

    • Author(s)
      鵜飼光子、川村翔栄、岸田敬吾、亀谷宏美
    • Journal Title

      日本調理科学会誌

      Volume: 46 Pages: 45-50

    • NAID

      110009579078

    • URL

      https://www.jstage.jst.go.jp/article/cookeryscience/46/1/46_45/_pdf

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] キノコ熱水抽出物のESRスピントラップ法によるラジカル捕捉活性評価とORAC法2013

    • Author(s)
      菅野友美、川村翔栄、原田栄津子、亀谷宏美、鵜飼光子、大澤俊彦
    • Journal Title

      日本食品科学工学会誌

      Volume: 60 Pages: 173-178

    • NAID

      10031163930

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photochemical method to evaluate oxidation levels in heat-treated vegetable oil: comparison with peroxide-value and acid-value2013

    • Author(s)
      Hiromi Kameya, Mitsuko Ukai
    • Journal Title

      Journal of Food Science and Engineering

      Volume: 3 Pages: 299-308

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple free-radical scavenging capacity in serum2012

    • Author(s)
      Shigeru Oowada, Nobuyuki Endo, Hiromi Kameya, Masashi Shimmei, Yashige Kotake
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 51 Issue: 2 Pages: 117-121

    • DOI

      10.3164/jcbn.11-113

    • NAID

      130004879294

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ESRスピントラップ法によるインスタントコーヒーのヒドロキシラジカル抗酸化評価2012

    • Author(s)
      亀谷宏美、鵜飼光子
    • Journal Title

      日本調理科学会誌

      Volume: 45 Pages: 33-36

    • URL

      https://www.jstage.jst.go.jp/article/cookeryscience/45/1/45_33/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 澱粉モノアシルグリセロール系における加熱生成ラジカルの解析2012

    • Author(s)
      伊藤友美、亀谷宏美、鵜飼光子
    • Journal Title

      日本調理科学会誌

      Volume: 45 Pages: 285-288

    • URL

      https://www.jstage.jst.go.jp/article/cookeryscience/45/4/45_285/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ESRスピントラップ法によるインスタントコーヒーのヒドロキシルラジカル抗酸化力評価2012

    • Author(s)
      亀谷宏美, 鵜飼光子
    • Journal Title

      日本調理科学会誌

      Volume: 45 Pages: 33-36

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 澱粉-モノアシルジグリセロール系における加熱生成ラジカルの解析2012

    • Author(s)
      伊藤友美, 亀谷宏美, 鵜飼光子
    • Journal Title

      日本調理科学会誌

      Volume: 45 Pages: 285-288

    • NAID

      110009480375

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] New ESR detection method of hydroxyl radical scavenging activity of blue berry2011

    • Author(s)
      Hiromi Kameya, Mitsuko Ukai
    • Journal Title

      Journal of Materials Science and Engineering

      Volume: 1 Pages: 347-351

    • URL

      http://www.davidpublishing.org/show.html?1550

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 食品を試料としたESRスピントラップ法での計測2013

    • Author(s)
      亀谷宏美
    • Organizer
      第1回MULTIS研究会
    • Place of Presentation
      同志社大学(京都府)
    • Year and Date
      2013-04-14
    • Related Report
      2013 Final Research Report
  • [Presentation] ESRスピントラップ法による光照射を用いたスーパーオキシドラジカル捕捉能計測方法2012

    • Author(s)
      亀谷宏美、川村翔栄、岸田敬吾、中村秀夫、鵜飼光子、等々力節子
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      藤女子大学(北海道)
    • Year and Date
      2012-08-28
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ESRを用いた加熱植物オイル中のフリーラジカル検討2012

    • Author(s)
      亀谷宏美、齊藤希巳江、等々力節子
    • Organizer
      日本食品化学会第18回総会・学術大会
    • Place of Presentation
      五島軒(北海道)
    • Year and Date
      2012-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂溶性スピントラップ剤CYPMPO誘導体の合成2012

    • Author(s)
      東谷裕之、亀谷宏美、鵜飼光子、亀谷宏美
    • Organizer
      日本化学会第92春季年回
    • Place of Presentation
      慶應義塾大学(神奈川県)
    • Year and Date
      2012-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂溶性スピントラップ剤CYPMPO 誘導体の合成2012

    • Author(s)
      東谷裕之, 亀谷宏美, 鵜飼光子, 中村秀夫
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] ESRスピントラップ法とORAC法による照射大豆の抗酸化性評価2011

    • Author(s)
      亀谷宏美、齊藤希巳江、等々力節子
    • Organizer
      日本食品科学工学会第58回大会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 食品を試料としたESRスピントラップ法での計測

    • Author(s)
      亀谷宏美
    • Organizer
      第1回MULTIS研究会
    • Place of Presentation
      同志社大学(京都府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ESRを用いた加熱植物オイル中のフリーラジカルの検討

    • Author(s)
      亀谷宏美, 齊藤希巳江, 等々力節子
    • Organizer
      日本食品化学会 第18回総会・学術大会
    • Place of Presentation
      五島軒(北海道)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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