| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Oxidative modification of LDL is one of the critical steps for the development of atherosclerosis. Previous study demonstrated that 12/15-lipoxygenase highly expressed in macrophages is essentially in the process of extracellular LDL oxidation. We evaluated the inhibitory effect of 12 Chinese teas on 12/15-lipoxygenase activity. Tea catechins such as epigallocatechin gallate have been known to exhibit 12/15-lipoxygenase inhibition. Qing Shan Lu Shui, which contains lower catechin levels than the other tested teas, suppressed 12/15-lipoxygenase activity. To identify component(s) that inhibited 12/15-lipoxygenase, we fractionated the extract by reverse-phase HPLC and found fractions containing a potent inhibitory activity. The components in the fractions were isolated and analyzed by electro spray-ionization mass spectrometry, 1H-NMR and 13C-NMR. The results suggested that these compounds were novel monoterpenoid glycosides.
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