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Search of foods inhibiting 12/15-lipoxygenase for prevention of atherosclerosis.

Research Project

Project/Area Number 23700916
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits, studies on eating habits
Research InstitutionOkayama Prefectural University

Principal Investigator

KAWAKAMI Yuki  岡山県立大学, 保健福祉学部, 助教 (30453202)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsリポキシゲナーゼ / 食品 / 動脈硬化
Research Abstract

Oxidative modification of LDL is one of the critical steps for the development of atherosclerosis. Previous study demonstrated that 12/15-lipoxygenase highly expressed in macrophages is essentially in the process of extracellular LDL oxidation. We evaluated the inhibitory effect of 12 Chinese teas on 12/15-lipoxygenase activity. Tea catechins such as epigallocatechin gallate have been known to exhibit 12/15-lipoxygenase inhibition. Qing Shan Lu Shui, which contains lower catechin levels than the other tested teas, suppressed 12/15-lipoxygenase activity. To identify component(s) that inhibited 12/15-lipoxygenase, we fractionated the extract by reverse-phase HPLC and found fractions containing a potent inhibitory activity. The components in the fractions were isolated and analyzed by electro spray-ionization mass spectrometry, 1H-NMR and 13C-NMR. The results suggested that these compounds were novel monoterpenoid glycosides.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (20 results)

  • [Journal Article] Two New Monoterpene Glycosides with Inhibitory Effects on Leukocyte-type 12-Lipoxygenase Activity from Qing Shan Lu Shui Tea2013

    • Author(s)
      Hideyuki Ito, Akemi Otsuki, Hitomi Mori, Peng Li, Mai Kinoshita, Yuki Kawakami, Hideaki Tsuji, Ding Zhi Fang, Yoshitaka Takahashi
    • Journal Title

      Molecules

      Volume: 18 Issue: 4 Pages: 4257-4266

    • DOI

      10.3390/molecules18044257

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antiatherogenic effect of guava leaf extracts inhibiting leucocyte-type 12-lipoxygenase activity2012

    • Author(s)
      Yuki Kawakami
    • Journal Title

      Food Chemistry

      Volume: 131 Pages: 1069-1075

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒドロペルオキシ基を持つ新規モノテルペン配糖体による白血球型12-リポキシゲナーゼの阻害2014

    • Author(s)
      川上祐生、大槻朱美、木下麻衣、森香子、山本登志子、辻英明、伊東秀之、 FANG Ding Zhi、高橋吉孝
    • Organizer
      日本過酸化脂質・抗酸化物質学会第21回年会
    • Place of Presentation
      仙台
    • Year and Date
      2014-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibitory effect of tea extracts on leukocyte-type 12-lipoxygenase activity2014

    • Author(s)
      Yuki Kawakami, Akemi Otsuki, Mai Kinoshita, Yoshiko Mori, Makiko Suzuki, Toshiko Suzuki-Yamamoto, Masumi Kimoto, Hideaki Tsuji, Hiromi Yamashita, Hideyuki Ito, Ding Zhi Fang, Yoshitaka Takahashi
    • Organizer
      The First Okayama Prefectural University International Workshop with Nanchang University on Foods and Nutritional Sciences
    • Place of Presentation
      Soja, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 白血球型12-リポキシゲナーゼを阻害する新規化合物の解明2013

    • Author(s)
      大槻朱美、森香子、木下麻衣、勝原加奈子、森ひとみ、李鵬、川上祐生、山本登志子、辻英明、伊東秀之、 Ding Zhi Fang、高橋吉孝
    • Organizer
      おかやまバイオアクティブ研究会第42回シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] リポキシゲナーゼ阻害による動脈硬化症予防を目指した研究2013

    • Author(s)
      川上祐生、山本登志子、高橋吉孝、伊東秀之
    • Organizer
      岡山県立大学OPUフォーラム2013
    • Place of Presentation
      総社
    • Year and Date
      2013-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] リポキシゲナーゼ阻害をターゲットにした生活習慣病予防に関する研究2013

    • Author(s)
      川上祐生、高橋吉孝
    • Organizer
      第8回県立研究機関協議会研究交流発表会
    • Place of Presentation
      赤磐
    • Year and Date
      2013-01-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibitory effect of Qing Shan Lu Shui Tea on leukocyte-type 12-lipoxygenase activity2013

    • Author(s)
      Yuki Kawakami, Akemi Otsuki, Yoshiko Mori, Hideyuki Ito, Toshiko Suzuki-Yamamoto, Hideaki Tsuji, Ding Zhi Fang, Yoshitaka Takahashi
    • Organizer
      ISNFF2013
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] 中国茶に含まれる白血球型12-リポキシゲナーゼ阻害成分の解析第86回日本生化学会2013

    • Author(s)
      大槻朱美、森香子、森ひとみ、李鵬、川上祐生、山本登志子、辻英明、伊東秀之、方定志、高橋吉孝
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規モノテルペン配糖体による白血球型12-リポキシゲナーゼ阻害2013

    • Author(s)
      川上祐生、大槻朱美、森香子、伊東秀之、山本登志子、辻英明、 Ding Zhi Fang、高橋吉孝
    • Organizer
      日本油化学会第52回年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] 白血球型12-リポキシゲナーゼに対する中国茶葉抽出物の阻害効果2013

    • Author(s)
      川上祐生、大槻朱美、平野詩織、木下麻衣、山本登志子、辻英明、木本眞順美、山下広美、伊東秀之、 Ding Zhi Fang、高橋吉孝
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Inhibitory effect of Qing Shan Lu Shui Tea on leukocyte-type 12-lipoxygenase activity2013

    • Author(s)
      Yuki Kawakami, Akemi Otsuki, Yoshiko Mori, Hideyuki Ito, Toshiko Suzuki-Yamamoto, Hideaki Tsuji, Ding Zhi Fang, Yoshitaka Takahashi
    • Organizer
      ISNFF2013
    • Place of Presentation
      Taipei
    • Related Report
      2013 Annual Research Report
  • [Presentation] 中国茶に含まれる白血球型12-リポキシゲナーゼ阻害成分の解析2013

    • Author(s)
      大槻朱美、森香子、森ひとみ、李鵬、川上祐生、山本登志子、辻英明、伊東秀之、方定志、高橋吉孝
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新規モノテルペン配糖体による白血球型12-リポキシゲナーゼ阻害2013

    • Author(s)
      川上祐生、大槻朱美、森香子、伊東秀之、山本登志子、辻英明、Ding Zhi Fang、高橋吉孝
    • Organizer
      日本油化学会 第52回年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 白血球型12リポキシゲナーゼ阻害作用を有する青山緑水の成分研究2012

    • Author(s)
      伊東秀之、大槻朱美、川上祐生、辻英明、 Ding Zhi Fang、高橋吉孝
    • Organizer
      第45回日本栄養・食糧学会中国・四国支部大会
    • Place of Presentation
      松山
    • Year and Date
      2012-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibitory effect of Chinese tea on leukocyte-type 12-lipoxygenase activity2012

    • Author(s)
      Akemi Otsuki, Shiori Hirano, Mai Kinoshita, Ichika Shimoda, Yuki Kawakami, Toshiko Suzuki-Yamamoto, Hideaki Tsuji, Hideyuki Ito, Ding Zhi Fang, Yoshitaka Takahashi
    • Organizer
      Bioactive Okayama 2012
    • Place of Presentation
      Okayama
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 生活習慣病予防を目指したリポキシゲナーゼ阻害食品の探索2012

    • Author(s)
      川上祐生、山本登志子、辻英明、高橋吉孝
    • Organizer
      OPUフォーラム 2012
    • Place of Presentation
      総社
    • Year and Date
      2012-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 中国茶に含まれる白血球型12リポキシゲナーゼ阻害成分の解析2012

    • Author(s)
      川上祐生、大槻朱美、平野詩織、木下麻衣、下田一花、山本登志子、辻英明、木本眞順美、伊東秀之、 Ding Zhi Fang、高橋吉孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 中国茶に含まれる白血球型12-リポキシゲナーゼ阻害成分の解析2012

    • Author(s)
      川上祐生、大槻朱美、平野詩織、木下麻衣、下田一花、山本登志子、辻英明、木本眞順美、伊東秀之、Ding Zhi Fang、高橋吉孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Inhibitory effect of the extracts from Psidium guajava on the catalytic activity of leukocyte-type 12-lipoxygenase2011

    • Author(s)
      Yuki Kawakami
    • Organizer
      ICoFF2011
    • Place of Presentation
      Taipei International Convention Cneter (Taipei)
    • Related Report
      2011 Research-status Report
  • [Presentation] グァバ葉抽出物に含まれる没食子酸エチルによる白血球型12-リポキシゲナーゼ阻害2011

    • Author(s)
      川上祐生
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒドロペルオキシ基を持つ新規モノテルペン配糖体による白血球型12-リポキシゲナーゼの阻害

    • Author(s)
      川上祐生、大槻朱美、木下麻衣、森香子、山本登志子、辻英明、伊東秀之、FANG Ding Zhi、高橋吉孝
    • Organizer
      日本過酸化脂質・抗酸化物質学会 第21回年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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