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Identification of food-derived peptide and its bioactivities after oral ingestion of hydrolysate prepared from connective tissues

Research Project

Project/Area Number 23700944
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits, studies on eating habits
Research InstitutionTokyo Kasei Junior College (2012)
Osaka Yuhigaoka Gakuen College (2011)

Principal Investigator

SHIGEMURA Yasutaka  東京家政大学短期大学部, 栄養科, 講師 (20373178)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsペプチド / エラスチン / Pro-Gly / 食事由来ペプチド / プロテオグリカン / サメ軟骨加水分解物 / 結合組織タンパク質 / タンパク質加水分解物 / コラーゲン / ヒドロキシプロリン
Research Abstract

In the present study, we identified prolyl-glycine (Pro-Gly) as the food-derived peptide in human blood after oral ingestion of elastin hydrolysate. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reverse phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 minutes after ingestion, and decreasing approximately20% at 4 hours after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (9 results) Book (5 results)

  • [Journal Article] Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly),in Human Blood after Ingestion of Elastin Hydrolysate.2012

    • Author(s)
      Shigemura, Y.; Nakaba, M.; Shiratsuchi,E.; Suyama, M.; Yamada, M.; Kiyono, T.;Fukamizu, K.; Park, E. Y.; Nakamura, Y.;Sato, K.
    • Journal Title

      Journal of agricultural and food chemistry

      Volume: 60 (20) Pages: 5128-5133

    • Related Report
      2012 Final Research Report
  • [Journal Article] ヒト血液中からの食事由来エラスチンペプチドの検出2012

    • Author(s)
      重村泰毅・佐藤健司
    • Journal Title

      食品加工技術

      Volume: 32(2) Pages: 10-18

    • NAID

      40019416291

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Identification of food-derived elastin peptide, prolyl-glycine (Pro-Gly), in human blood after ingestion of elastin hydrolysate2012

    • Author(s)
      Shigemura,Y., Nakaba, M., Shiratsuchi, E., Suyama, M., Yamada, M., Kiyono, T. Fukamizu, K., Park, E. Y., Nakamura, Y., Sato, K.
    • Journal Title

      J. Agric. Food Chem.

      Volume: 60 Issue: 20 Pages: 5128-5133

    • DOI

      10.1021/jf300497p

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a Novel Food-Derived Collagen Peptide, Hydroxyprolyl-Glycine, in Human Peripheral Blood by Pre-column Derivatization with Phenyl Isothiocyanate2011

    • Author(s)
      Shigemura Y, Akaba S, Kawashima E, Park EY, Nakamura Y, Sato K.
    • Journal Title

      Food Chemistry

      Volume: 129 Issue: 3 Pages: 10191024-10191024

    • DOI

      10.1016/j.foodchem.2011.05.066

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] An Evaluation of Protein Intake for Metabolic Demands and the Quality of Dietary Protein in Rats Using an Indicator Amino Acid Oxidation Method2011

    • Author(s)
      Ogawa A, Naruse Y, Shigemura Y, Kobayashi Y, Suzuki I, Wada S, Hayamizu K, Kuwahata M, Kido Y.
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 57 Issue: 6 Pages: 418-425

    • DOI

      10.3177/jnsv.57.418

    • NAID

      130002103052

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Kenji Sato Effect of ingestion of elastin hydrolysate on skin condition2012

    • Author(s)
      Yasutaka Shigemura, Eri Shiratsuchi
    • Organizer
      ISNFF 2012
    • Place of Presentation
      サンフランシスコハワイ州コナ
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of ingestion of elastin hydrolysate on skin condition2012

    • Author(s)
      Yasutaka Shigemura, Eri Shiratsuchi, Kenji Sato
    • Organizer
      ISNFF 2012
    • Place of Presentation
      ハワイ州 コナ
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト血中における食事由来大豆ペプチドの検出2012

    • Author(s)
      木村 桂太、重村 泰毅、宮地 美里、朴 恩榮、中村 考志、佐藤 健司
    • Organizer
      第51回栄養食糧学会 近畿支部大会
    • Place of Presentation
      甲子園大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト血液中食事由来大豆ペプチドの検出2012

    • Author(s)
      重村泰毅、深水和菜、清野珠美、木村桂太、中森俊宏、馬場俊充、前渕元宏、朴恩榮、中村考志、佐藤健司
    • Organizer
      第59回食品科学工学会
    • Place of Presentation
      藤女子大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 佐藤健司 ヒト血液中の食事由来エラスチンペプチド検出とそのペプチドが及ぼす線維芽細胞への影響2011

    • Author(s)
      重村泰毅 、白土絵理、深水和菜、朴恩榮、中村考志
    • Organizer
      第58回食品科学工学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 摂取期間と被験者年齢がおよぼす血液中食事由来コラーゲンペプチド濃度と組成への影響2011

    • Author(s)
      重村泰毅、深水和菜、川口友彰、黒川美保子、朴恩榮、中村考志、佐藤健司
    • Organizer
      栄養食糧学会 近畿支部大会
    • Place of Presentation
      近畿大学 奈良
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒト血液からの食事由来エラスチンペプチドの検出2011

    • Author(s)
      重村泰毅
    • Organizer
      美容・アンチエイジング食品研究会&機能性食品用ペプチド研究会 合同研究会(招待講演)
    • Place of Presentation
      大阪国際会議場 大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒト血液中の食事由来エラスチンペプチド検出とそのペプチドが及ぼす線維芽細胞への影響2011

    • Author(s)
      重村泰毅、白土絵理、深水和菜、朴恩榮、中村考志、佐藤健司
    • Organizer
      第58回食品科学工学会
    • Place of Presentation
      東北大学 仙台
    • Related Report
      2011 Research-status Report
  • [Presentation] コラーゲンペプチドの4週間連続摂取によるヒト血中食事由来ペプチドの組成変化2011

    • Author(s)
      重村泰毅・川口友彰・黒川美保子・朴恩榮・中村考志・佐藤健司
    • Organizer
      第65回日本栄養食糧学会
    • Place of Presentation
      御茶ノ水大学 東京
    • Related Report
      2011 Research-status Report
  • [Book] Marine Proteins and Peptides: Biological Activities and Applications2013

    • Author(s)
      Eri Shiratsuchi, Misako Nakaba, Yasutaka Shigemura, Michio Yamada and Kenji Sato
    • Total Pages
      816
    • Publisher
      Wiley-Blackwell
    • Related Report
      2012 Annual Research Report
  • [Book] 食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―  第一章 6 機能性ペプチド探索のための新しいアプローチ2011

    • Author(s)
      重村泰毅
    • Total Pages
      6
    • Publisher
      CMC出版
    • Related Report
      2011 Research-status Report
  • [Book] Marine Cosmeceuticals: Trends and Prospects, (chapter 10) Effect of MarineCollagen peptide on skin condition.2011

    • Author(s)
      Kenji Sato, Yasutaka Shigemura
    • Total Pages
      8
    • Publisher
      CRC PRESS
    • Related Report
      2011 Research-status Report
  • [Book] 栄養科学シリーズNEXT「栄養生理学・生化学実験」第2章 基本操作と測定原理2011

    • Author(s)
      重村泰毅
    • Total Pages
      8
    • Publisher
      講談社
    • Related Report
      2011 Research-status Report
  • [Book] Wiley-Blackwell. Marine Proteins and Peptides: Biological Activities and Applications.

    • Author(s)
      Eri Shiratsuchi,Misako Nakaba, Yasutaka Shigemura, Michio Yamada and Kenji Sato
    • Publisher
      Fish elastin hydrolysate-Development of product and its impact on skin and blood vessel.
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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