Identification of food-derived peptide and its bioactivities after oral ingestion of hydrolysate prepared from connective tissues
| Project/Area Number |
23700944
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Tokyo Kasei Junior College (2012)
Osaka Yuhigaoka Gakuen College (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ペプチド / エラスチン / Pro-Gly / 食事由来ペプチド / プロテオグリカン / サメ軟骨加水分解物 / 結合組織タンパク質 / タンパク質加水分解物 / コラーゲン / ヒドロキシプロリン |
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| Research Abstract |
In the present study, we identified prolyl-glycine (Pro-Gly) as the food-derived peptide in human blood after oral ingestion of elastin hydrolysate. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reverse phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 minutes after ingestion, and decreasing approximately20% at 4 hours after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly),in Human Blood after Ingestion of Elastin Hydrolysate.2012
Author(s)
Shigemura, Y.; Nakaba, M.; Shiratsuchi,E.; Suyama, M.; Yamada, M.; Kiyono, T.;Fukamizu, K.; Park, E. Y.; Nakamura, Y.;Sato, K.
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Journal Title
Journal of agricultural and food chemistry
Volume: 60 (20)
Pages: 5128-5133
Related Report
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[Journal Article] Identification of food-derived elastin peptide, prolyl-glycine (Pro-Gly), in human blood after ingestion of elastin hydrolysate2012
Author(s)
Shigemura,Y., Nakaba, M., Shiratsuchi, E., Suyama, M., Yamada, M., Kiyono, T. Fukamizu, K., Park, E. Y., Nakamura, Y., Sato, K.
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Journal Title
J. Agric. Food Chem.
Volume: 60
Issue: 20
Pages: 5128-5133
DOI
Related Report
Peer Reviewed
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