Establishment of the primary culture of precursor grand in genetical engineering mice

• Project/Area Number: 23701042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Asahikawa Medical College
• Principal Investigator: SASAJIMA Junpei 旭川医科大学, 大学病院, 医員 (80451467)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 前癌病変 / 膵臓癌 / マイクロRNA / 遺伝子改変マウス / 3次元培養 / ３次元培養 / マイクロＲＮＡ

Research Abstract

This study established the methods of isolation of precoursor gland from genetical engineering mice. Subject to differentiation of isolated gland, ductal morphology was observed in the three dimension culture system. We detected candidates of micro RNA which accelerate or suppress its carcinogenesis.