Functional intravital imaging of the breast tumor invasion process in tumor microenvironment
| Project/Area Number |
23701053
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kyoto University |
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Principal Investigator |
KAMIOKA Yuji 京都大学, 医学(系)研究科(研究院), 助教 (50511424)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | FRET / 蛍光共鳴エネルギー移動 / イメージング / 癌微小環境 / 生体イメージング / ERK / PKA / 低分子量Gタンパク質 / Rhoファミリー / 乳癌 / 国際情報交流 |
|---|
| Research Abstract |
A variety of fluorescent biosensors based on the principle of Fluorescence Resonance Energy Transfer (FRET) have been well used to visualize the activities of various signaling molecules in vitro. However, living tissues actually consist of many kinds of cells and cellular interactions in three-dimensional environments.
Recent studies show the importance of tumor microenvironment comprising of tumor cells and stroma cells. In this project, molecular activities of ERK, PKA and Rac1 were visualized by using genetically-encoded FRET biosensors in living mice under two-photon microscope for the understanding of tumor microenvironment in vivo. We have developed transgenic mice which express FRET biosensors for ERK and PKA in this project. In addition, imaging tools, stabilizer and windows were developed to get motion-less images and keep mice alive. These results achieved by intravital imaging can progress the studies about tumor microenvironment.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo.2014
Author(s)
Kagawa Y, Matsumoto S, Kamioka Y, Mimori M, Naito Y, Ishii T, Okuzaki D, Nishida N, Maeda S, Naito A, Kikuta J, Nishikawa K, Nishimura J, Haraguchi N, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Ishii H, Doki Y, Matsuda M, Kikuchi A, Mori M, Ishii M
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Journal Title
PLoS One
Volume: 8
Issue: 12
Pages: e83629-e83629
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors2012
Author(s)
Kamioka, Y., Sumiyama, K., Mizuno, R., Sakia, Y., Hirata, E., Kiyokawa, E., Matsuda, M.
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Journal Title
Cell Structure and Function
Volume: 37
Issue: 1
Pages: 65-73
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed
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