| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
MicroRNAs (miRNAs) are endogenously expressed small non-coding RNAs that function as negative regulators of gene expression. Posttranscriptional regulation by miRNAs is important for many aspects of development and disease. Sveral miRNAs are highly expressed in endothelial cells. Whereas the specific functions of miRNAs for tumor angiogenesis are less clear.
Here, we identified miRNAs that were enriched in endothelial cells derived from LLC tumor. We found that miR-125b regulated the expression of endothelial cell adherent protein VE-cadherin. Additionally, overexpression of miR-125b in endothelial cells resulted in inhibition of proliferation, tube formation and migration activity. Furthermore, in vivo transfection of mir-125b in subcutaneous implanted LLC tumor, inhibited neo vascular formation and suppressed tumor growth.
These findings illustrate that mir-125b act as negative regulator of tumor angiogenesis and providing a new target for modulating vascular formation and function.
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