Roles of laminin g2 chain in tumor growth and invasion.
| Project/Area Number |
23701059
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | がん微小環境 / ラミニン / プロセシング / がん / 浸潤 / ガレクチン / ECM / 細胞内シグナル |
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| Research Abstract |
To analyze the effect of Lm g2 chain monomer on tumor growth and invasion in vivo, we overexpressed of the g2 chain in the human bladder carcinoma cell line EJ-1 and human embryonic kidney cell line HEK-293. When implanted into nude mice subcutaneously or intraperitoneally, gamma2 overexpressed cells grew faster and produced much larger solid tumors than the control transfectant. These data strongly suggest that the g2 monomer contribute to the invasive growth of tumor cells in stromal tissues. Next, we overexpressed three different 2 flagments in the HEK-293 and analyzed the active site of the 2 chain. It was found that domein V of 2 chain (2dV) strongly induced the tumor growth. Then, we established monoclonal antibodies against the domain V of human laminin 2 chain. Further detailed characterization of the antibody is in progress.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Self-association of the galectin-9 C-terminal domain via the opposite surface of the sugar-binding site2013
Author(s)
Nonaka, Y., Ogawa, T., Oomizu, S., Nakakita, S., Nishi, N., Kamitori, S., Hirashima, M. & Nakamura, T
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Journal Title
J. Biochem
Volume: 153
Issue: 5
Pages: 463-471
DOI
Related Report
Peer Reviewed
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