CD133-responsive conditionally replicating adenovirus targeting cancer stem cells

• Project/Area Number: 23701062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Kagoshima University
• Principal Investigator: WANG Yuqing 鹿児島大学, 医歯(薬)学総合研究科, 特任研究員 (20505143)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腫瘍生物学 / 癌幹細胞 / 遺伝子治療 / アデノウイルス / 国際研究者交流 / アデノウイルスベクター

Outline of Final Research Achievements

Cancer stem cells has been note as an important therapeutic target in cancer, and gene therapy such as by virotherapy focused on cancer stem cell maybe provides a novel therapeutic approach to anticancer. CD133 is known as a cancer stem marker expressed in nervous system tumors, colon cancer and prostate cancer, etc. A novel conditionally replicating adenovirus vector CD133pr.m-CRA was constructed and showed cancer stem cell-specific therapy effect in vitro and in vivo tumor model without toxicity to normal cells. Our date indicate that CD133.m-CRAs is a useful strategy for treating cancer stem cells.

Research Products

• [Journal Article] Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny (2014)
  • Author(s): Tanoue K, Wang Y, Ikeda M , Mitsui K, Irie R , Setoguchi T, Komiya S, Natsugoe S, Kosai K
  • Journal Title: J Trans Med Volume: 12 Issue: 1 Pages: 27-27
  • DOI: 10.1186/1479-5876-12-27
  • Peer Reviewed / Open Access
• [Presentation] Immunohistochemical comparative analysis of the sphere cells of CD133-positive pancreatic cancer cells with iPS cells. (2013)
  • Author(s): 宮崎 優美、王 宇清、三井 薫、丁 強、政 幸一郎、松原 修一郎、小戝 健一郎、高尾 尊身。
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜）
• [Presentation] Survivin-responsive conditionally replicating adenovirus efficiently kills rhabdomyosarcoma-initiating cells: a promising m-CRA strategy for treating cancer stem cells. (2013)
  • Author(s): 王 宇清、田上 聖徳、池田 美奈子、小戝 健一郎等。
  • Organizer: がん若手研究者ワークショップ
  • Place of Presentation: 蓼科グランドホテル滝の湯(長野)
• [Presentation] 独自開発のサバイビン依存性増殖型アデノウイルスベクターは横紋筋肉腫幹細胞に対して有効な治療薬として期待できる。 (2012)
  • Author(s): 田上 聖徳、王 宇清、瀬戸口 啓夫、小戝 健一郎等
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] ヒトES/iPS細胞での再生医療の課題を克服する独自のアデノウイルスベクターと発現技術の開発。 (2012)
  • Author(s): 小戝 健一郎、三井 薫、王 宇清、高橋 知之
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 独自開発の増殖型アデノウイルスベクターで癌幹細胞は効果的に治療できる。 (2012)
  • Author(s): 田上 聖徳、王 宇清、池田 美奈子、小戝 健一郎等
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] Survivin-responsive conditionally replicating adenovirus efficiently kills rhabdomyosarcoma-initiating cells: a promising m-CRA strategy for treating cancer stem cells. (2012)
  • Author(s): Kiyonori Tanoue, Yuqing Wang, Minako Ikeda, Ken-ichiro Kosai, et al.
  • Organizer: 第18回日本遺伝子治療学会
  • Place of Presentation: 熊本
• [Presentation] Efficient treatment of Rhabdomyosarcoma-initiating cells by surviving-responsive conditionally replicating adenovirus: Promising m-CRA strategy for treating cancer stem cells. (2012)
  • Author(s): Kiyonori Tanoue, Yuqing Wang, Minako Ikeda, Ken-ichiro Kosai, et al.
  • Organizer: American Society of Gene & Cell Therapy (ASGCT) 15th Annual Meeting
  • Place of Presentation: Philadelphia, アメリカ合衆国
• [Patent(Industrial Property Rights)] 癌幹細胞を標的とするウイルスベクター (2013)
  • Inventor(s): 小戝 健一郎、王 宇清
  • Industrial Property Rights Holder: 小戝 健一郎、王 宇清
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-09-24
  • Overseas