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Functional analysis of phosphoinositides in invasion and metastasis of breast cancer cells

Research Project

Project/Area Number 23701069
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionNational Cancer Center Japan

Principal Investigator

YAMAGUCHI Hideki  独立行政法人国立がん研究センター, 研究所, ユニット長 (10345035)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords浸潤 / 癌 / 浸潤突起 / イノシトールリン脂質
Research Abstract

Invadopodia are membrane protrusions formed by invasive cancer cells that mediate degradation of pericellular ECM and have been implicated in local invasion during cancer metastasis. In this study, we investigated the role of phosphoinositide metabolism in invadopodium formation. We found that PI3 kinase p110α, a frequently mutated gene product in human cancers, is involved in invadopodium formation and invasion by breast cancer cells. This finding suggests that the signaling pathway via p110α is a potential therapeutic target for the treatment of cancer invasion and metastasis.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] CDCP1 regulates the function of MT1-MMP and invadopodia-mediated invasion of cancer cells2013

    • Author(s)
      Miyazawa Y
    • Journal Title

      Mol. Cancer Res.

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidermal phospholipase C δ1 regulates granulocyte counts and systemic interleukin-17 levels in mice.2012

    • Author(s)
      Kaori Kanemaru
    • Journal Title

      Nat. Commun.

      Volume: 3 Issue: 1 Pages: 963-963

    • DOI

      10.1038/ncomms1960

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pathological roles of invadopodia in cancer invasion and metastasis.2012

    • Author(s)
      Yamaguchi H
    • Journal Title

      Eur. J. Cell. Biol.

      Volume: 91 Pages: 902-907

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-WASP-mediated invadopodium formation is necessary for intravasation and lung metastasis of mammary tumors.2012

    • Author(s)
      Gligorijevic B, Wyckoff J, Yamaguchi H, Wang Y, Roussos E, and Condeelis J
    • Journal Title

      J. Cell Sci.

      Volume: 125 Pages: 724-734

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] がん細胞による浸潤突起形成の分子機構2012

    • Author(s)
      山口英樹
    • Journal Title

      生化学.日本生化学会

      Volume: Vol.84

    • NAID

      10030346185

    • Related Report
      2012 Final Research Report
  • [Journal Article] Pathological roles of invadopodia in cancer invasion and metastasis2012

    • Author(s)
      Yamaguchi H
    • Journal Title

      Eur. J. Cell. Biol.

      Volume: 91 Issue: 11-12 Pages: 902-907

    • DOI

      10.1016/j.ejcb.2012.04.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-WASP-mediated invadopodium formation is necessary for intravasation and lung metastasis of mammary tumors2012

    • Author(s)
      Gligorijevic B, Wyckoff J, Yamaguchi H, Wang Y, Roussos E, Condeelis J
    • Journal Title

      Journal of Cell Science

      Volume: 125 Issue: 3 Pages: 724-734

    • DOI

      10.1242/jcs.092726

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] がん細胞による浸潤突起形成の分子機構2012

    • Author(s)
      山口英樹
    • Journal Title

      生化学

      Volume: 84 Pages: 35-38

    • NAID

      10030346185

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
  • [Journal Article] PI3-kinase signaling pathway mediated by p110α regulates invadopodia formation.2011

    • Author(s)
      Yamaguchi H, Yoshida S, Muroi E, Yoshida N, Kawamura M, Yoshida N, Kouchi Z, Nakamura Y, Sakai R, and Fukami K
    • Journal Title

      J. Cell. Biol.

      Volume: 193 Pages: 1275-1288

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic defect in phospholipase Cδ1 protects mice from obesity by regulating thermogenesis and adipogenesis.2011

    • Author(s)
      Hirata M, Suzuki M, Ishii R, Kitazumi T, Sasaki T, Kitamura T, Yamaguchi H, Nakamura Y, and Fukami K
    • Journal Title

      Diabetes

      Volume: 60 Pages: 1926-1937

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 5-phosphate 4-kinase IIbeta promotes vitamin D receptor-dependent E-cadherin expression in SW480 cells.2011

    • Author(s)
      Kouchi Z, Fujiwara Y, Yamaguchi H, Nakamura Y, and Fukami K
    • Journal Title

      Biochem. Biophys. Res. Comm.

      Volume: 408 Pages: 523-529

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase signaling and neurite outgrowth.2011

    • Author(s)
      Kouchi Z, Igarashi T, Shibayama N, Inanobe S, Sakurai K, Yamaguchi H, Fukuda T, Yanagi S, Nakamura Y, and Fukami K
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 8459-8471

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phospholipase Cd3 is a novel binding partner of Myosin VI and functions as anchoring of Myosin VI on plasma membrane.2011

    • Author(s)
      Sakurai K, Hirata M, Yamaguchi H, Nakamura Y, and Fukami K
    • Journal Title

      Advan. Enzyme Regul.

      Volume: 51 Pages: 171-181

    • Related Report
      2012 Final Research Report
  • [Journal Article] Phosphoinositide 3-kinase signaling pathway mediated by p110α regulates invadopodia formation2011

    • Author(s)
      Yamaguchi H, Yoshida S, Muroi E, Yoshida N, Kawamura M, Kouchi Z, Nakamura Y, Sakai R, Fukami K
    • Journal Title

      J.Cell Biol.

      Volume: 193 Issue: 7 Pages: 1275-1288

    • DOI

      10.1083/jcb.201009126

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] CDCP1 regulates the function of MT1-MMP and invadopodia-mediated invasion of cancer cells. Mol.

    • Author(s)
      Miyazawa Y, Uekita T, Ito Y, Seiki M, Yamaguchi H, and Sakai R
    • Journal Title

      Cancer Res.

      Volume: (In press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Differential sensitivities to molecular target drugs in scirrhous gastric carcinoma cell lines2013

    • Author(s)
      Yamaguchi H, Yoshida N, Takanashi M, and Sakai R
    • Organizer
      The 9th AACR-Japanese Cancer Association Joint Conference: Breakthroughs in Basic and Translational Cancer Research
    • Place of Presentation
      Maui, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of PI3-kinase signaling pathway in invadopodia formation2013

    • Author(s)
      Yamaguchi H, Yoshida N, and Sakai R
    • Organizer
      AACR Special Conference of Tumor Invasion and Metastasis
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] スキルス胃癌細胞と間質線維芽細胞の相互作用による細胞外基質リモデリングと浸潤2012

    • Author(s)
      山口英樹、吉田那智、高梨美帆、深見希代子、柳原五吉、八代正和、堺隆一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] スキルス胃癌細胞株におけるc-Met 受容体型チロシンキナーゼ依存性の検討2012

    • Author(s)
      山口英樹、柳原五吉、堺隆一
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] スキルス胃癌細胞におけるMet シグナル伝達系の機能解析2012

    • Author(s)
      山口英樹、堺隆一
    • Organizer
      第21回日本がん転移学会学術集会・総会
    • Place of Presentation
      広島
    • Related Report
      2012 Final Research Report
  • [Presentation] Cancer-associated fibroblasts mediate extracellular matrix remodeling and three-dimensional invasion of scirrhous gastric carcinoma cells2012

    • Author(s)
      山口英樹、柳原五吉、八代正和、堺隆一
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Cancer-associated fibroblasts mediate extracellular matrix remodeling and three-dimensional invasion of scirrhous gastric carcinoma cells2012

    • Author(s)
      Yamaguchi H, Yanagihara K, Yashiro M, and Sakai R
    • Organizer
      103rd Annual meeting of the American Association for Cancer Research
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of PI3-kinase signaling pathway in invadopodia formation2011

    • Author(s)
      Yamaguchi H
    • Organizer
      Podosomes, Invadopodia, and Focal Adhesions in Physiology and Pathology
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] 腫瘍由来線維芽細胞との共培養によるスキルス胃癌浸潤機構の解析2011

    • Author(s)
      山口英樹、柳原五吉、八代正和、堺隆一
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞膜脂質による癌細胞浸潤の制御機構2011

    • Author(s)
      山口英樹、深見希代子、堺隆一
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] スキルス胃癌細胞の胃癌由来線維芽細胞を介した細胞外基質リモデリングと浸潤2011

    • Author(s)
      山口英樹、堺隆一
    • Organizer
      第20回日本がん転移学会学術集会・総会
    • Place of Presentation
      浜松
    • Related Report
      2012 Final Research Report
  • [Presentation] Phosphoinositide 3-kinase signaling pathway mediated by p110alpha regulates invadopodia formation2011

    • Author(s)
      Yamaguchi H, Fukami K, and Sakai R
    • Organizer
      102nd Annual meeting of the American Association for Cancer Research
    • Place of Presentation
      Orlando, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Phosphoinositide 3-kinase signaling pathway mediated by p110α regulates invadopodia formation2011

    • Author(s)
      山口英樹
    • Organizer
      第102回米国がん学会大会
    • Place of Presentation
      オーランド、米国
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞膜脂質による癌細胞浸潤の制御機構2011

    • Author(s)
      山口英樹
    • Organizer
      第63回日本細胞生物学会大会(招待講演)
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] The role of PI3-kinase signaling pathway in invadopodia formation2011

    • Author(s)
      山口英樹
    • Organizer
      Podosomes, Invadopodia, and Focal Adhesions in Physiology and Pathology(招待講演)
    • Place of Presentation
      マドリード、スペイン
    • Related Report
      2011 Research-status Report
  • [Presentation] The role of PI3-kinase signaling pathway in invadopodia formation

    • Author(s)
      Yamaguchi H
    • Organizer
      AACR Special Conference of Tumor Invasion and Metastasis
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncc.go.jp/jp/nccri/divisions/07grow/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.ncc.go.jp/jp/nccri/divisions/07grow/07grow.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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