Analysis for crosstalk between estrogen and Wnt signalings

• Project/Area Number: 23701070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2012); National Institutes of Natural Sciences Okazaki Research Facilities (2011)
• Principal Investigator: MIYAGAWA Shinichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (30404354)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 女性ホルモン / Wnt / 女性生殖器官 / 女性生殖器

Research Abstract

An integrated network of endocrine chemicals (hormones) modulates the reproductive systems in most animals. Estrogens, one class of these endocrine hormones, are responsible for the induction of behavioral and physiological processes involving many organ systems. The female reproductive tract provides the sites for gamete fertilization, implantation and subsequent development of the embryo and delivery of the fetus. Organs in the female reproductive tract are also involved in women’s health issues including and tumorigenesis. Growth factors and ER signaling cooperate to play essential roles in cell proliferation, differentiation and tumor progression in mouse reproductive organs. Treatment of neonatal mice with diethylstilbestrol (DES) induces an estrogen-independent persistent proliferation and cornification of the vaginal epithelium, which typically results in cancerous lesions later in life. However, the mechanisms of the estrogen-dependent and -independent pathways had remained essentially unknown. We found that Wnt/beta-catenin signaling is involved in estrogen-independent phenomena in mouse vagina developmentally exposed to estrogens.。

Research Products

• [Journal Article] Sequential changes in the expression of Wnt- and Notch-related genes in the vagina and uterus of ovariectomized mice after estrogen exposure (2012)
  • Author(s): Nakamura T, Miyagawa S, Katsu Y, Sato T, Iguchi T, Ohta Y
  • Journal Title: In Vivo Volume: 26 Pages: 899-906
  • Peer Reviewed
• [Journal Article] P21 and Notch signalings in the persistently altered vagina induced by neonatal diethylstilbestrol exposure in mice (2012)
  • Author(s): Nakamura T, Miyagawa S, Katsu Y, Mizutani T, Sato T, Takeuchi T, Iguchi T, Ohta Y
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 1589-1595
  • NAID: 130001879705
  • Peer Reviewed
• [Journal Article] WNT family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice (2012)
  • Author(s): Nakamura T, Miyagawa S, Katsu Y, Watanabe H, Mizutani T, Sato T, Morohashi KI, Takeuchi T, Iguchi T, Ohta Y
  • Journal Title: Toxicology Volume: 296 Pages: 13-19
  • Peer Reviewed
• [Journal Article] p21 and Notch signalings in the persistently altered vagina induced by neonatal diethylstilbestrol exposure in mice (2012)
  • Author(s): Nakamura et al.
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 1589-1595
  • Peer Reviewed
• [Journal Article] Wnt family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice. (2012)
  • Author(s): Nakamura T, Miyagawa S, Katsu Y, Watanabe H, Mizutani T, Sato T, Morohashi KI, Takeuchi T, Iguchi T, Ohta Y
  • Journal Title: Toxicology Volume: 296 Issue: 1-3 Pages: 13-19
  • DOI: 10.1016/j.tox.2012.02.010
  • Peer Reviewed
• [Journal Article] The role of sonic hedgehog-gli2 pathway in the masculinization of external genitalia (2011)
  • Author(s): Miyagawa S, Matsumaru D, Murashima A, Omori A, Satoh Y, Haraguchi R, Motoyama J, Iguchi T, Nakagata N, Hui CC, Yamada G
  • Journal Title: Endocrinology Volume: 152 Pages: 2894-2903
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of induction of persistent changes by estrogenic chemicals on female reproductive tracts and external genitalia (2011)
  • Author(s): Miyagawa S, Sato M, Iguchi T
  • Journal Title: J Steroid Biochem Mol Biol Volume: 127 Pages: 51-57
  • Peer Reviewed
• [Journal Article] Essential functions of androgen signaling emerged through the developmental analysis of vertebrate sex characteristics (2011)
  • Author(s): Ogino Y, Miyagawa S, Katoh H, Prins GS, Iguchi T, Yamada G
  • Journal Title: Evol Dev Volume: 13 Pages: 315-325
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of induction of persistent ch (2011)
  • Author(s): Miyagawa, S. et al.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: 127 Pages: 51-57
  • Peer Reviewed
• [Journal Article] The role of sonic hedgehog-gli2 pathway in the mas (2011)
  • Author(s): Miyagawa, S. et al.
  • Journal Title: Endocrinology Volume: 152 Pages: 2894-2903
  • Peer Reviewed
• [Journal Article] Essential functions of androgen signaling emerged (2011)
  • Author(s): Ogino, Y., Miyagawa, S. et al.
  • Journal Title: Evo Dev Volume: 13 Pages: 315-325
  • Peer Reviewed
• [Presentation] Indispensable roles of estrogen receptor a for persistent vaginal change induced by neonatal 5a-dihydrotestosterone exposure (2012)
  • Author(s): Miyagawa S., Ohta Y., Iguchi T
  • Organizer: Gordon Research Conference
  • Place of Presentation: ウェストドーバー、バーモント州、アメリカ
  • Year and Date: 2012-06-05
• [Presentation] Indispensable roles of estrogen receptor α for persistent vaginal change induced by neonatal 5α-dihydrotestosterone exposure (2012)
  • Author(s): Miyagawa er al.
  • Organizer: Gordon Research Conference
  • Place of Presentation: Mount Snow Resort (アメリカバーモント州)
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html
• [Remarks] Iguchi Lab on Web
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html