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Identification of CTL epitope using mass spectrometer.

Research Project

Project/Area Number 23701079
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor immunology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

OKAMURA Ayako  愛知県がんセンター(研究所), 腫瘍免疫学部, 研究員 (10546948)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords腫瘍免疫 / 細胞傷害性Tリンパ球 / オートファジー / 抗原提示 / がん / 免疫 / CTL / エピトープ / マススペクトロメトリー
Research Abstract

Cytotoxic T lymphocytes (CTLs) exert anti-tumor effects through recognition of tumor antigen-derived peptides bound to human leukocyte antigens (HLAs) on cell surfaces. To identify diverse CTL epitopes of tumor antigens, mass spectrometry-based approach are examined. To investigate CTL epitope panel through tumor-specific antigen presenting machinery, MCF10A cells were transduced mutated K-ras gene. As a result, the gene-modified MCF10A cells had constitutively active autophagosomes and produced autophagosome-dependent epitopes. These data suggest that the cells having tumor-specific antigen presenting machinery provide us information on various CTL epitopes processed through the machinery.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results)

  • [Journal Article] Autophagy creates a CTL epitope that mimics tumor-associated antigens.2012

    • Author(s)
      Demachi-Okamura A
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 10 Pages: e47126-e47126

    • DOI

      10.1371/journal.pone.0047126

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 人工抗原提示細胞システムを利用した新規腫瘍抗原探索2012

    • Author(s)
      岡村文子
    • Organizer
      第34回日本造血細胞移植学会総会
    • Place of Presentation
      大阪、大阪国際会議場
    • Year and Date
      2012-02-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 人工抗原提示細胞システムを利用した新規腫瘍抗原探索2012

    • Author(s)
      岡村文子
    • Organizer
      第34回日本造血細胞移植学会総会(招待講演)
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 内在性HLAの発現を抑制し目的のHLA-A24を発現する人工抗原提示細胞を用いた卵巣がんを障害するCTL の誘導2011

    • Author(s)
      近藤紳司
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋、名古屋国際会議場
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 膵がん細胞における恒常的高活性オートファジーによるCTLエピトープの産生2011

    • Author(s)
      岡村文子
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋、名古屋国際会議場
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Artificial antigen presenting cells as tools for defining cancer-specific antigens2011

    • Author(s)
      葛島清隆
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋、名古屋国際会議場
    • Year and Date
      2011-10-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 内在性HLAの発現を抑制し目的のHLA-A24を発現する人工抗原提示細胞を用いた卵巣がんを障害するCTLの誘導2011

    • Author(s)
      近藤紳司
    • Organizer
      第15回日本がん免疫学会総会
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Year and Date
      2011-07-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 内因性HLAの発現を抑制し目的のHLA-A24を発現する人工抗原提示細胞を用いた卵巣癌を傷害するCTLの誘導2011

    • Author(s)
      近藤紳司
    • Organizer
      日本がん免疫学会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Related Report
      2011 Research-status Report
  • [Presentation] Induction of cancer-specific CTL using aAPCs expressing an extrinsic HLA of interest while silencing intrinsic ones2011

    • Author(s)
      近藤紳司
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] Artificial antigen presenting cells as tools for defining cancer-spcific antigens2011

    • Author(s)
      葛島清隆
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] Constitutively active autophagy causes CTL epitope generation in pancreatic cancer cells2011

    • Author(s)
      岡村文子
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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