Novel multi-omics approach for discovery of splicing variants in cancer stem cells

• Project/Area Number: 23701092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Shizuoka Cancer Center Research Institute
• Principal Investigator: HATAKEYAMA Keiichi 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (20564157)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 癌 / バイオマーカー / オミクス / がん幹細胞 / スプライシング / Splicing variant / Proteomics / Transcriptmics / Cancer stem cell

Research Abstract

This research aim is development of novel multi-omics approach for discovery of splicing variants in cancer stem cells. Our approach is based on transcriptomics and proteomics. Using the multi-omics analysis, we identified novel cancer-specific aberrant transcripts (splice variants) and variant-derived proteins (protein isoforms) in cancer cells and patients with cancer.

Research Products

• [Journal Article] Novel protein isoforms of carcinoembryonic antigen are secreted from pancreatic, gastric and colorectal cancer cells (2013)
  • Author(s): Hatakeyama K, Wakabayashi-Nakao K, Ohshima K, Sakura N, Yamaguchi K, Mochizuki T
  • Journal Title: BMC Res. Notes Volume: 6 Issue: 1 Pages: 381-381
  • DOI: 10.1186/1756-0500-6-381
  • Peer Reviewed
• [Journal Article] Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells. (2013)
  • Author(s): Hatakeyama K, Fukuda Y, Ohshima K, Terashima M, Yamaguchi K, MochizukiT
  • Journal Title: BMC Res Notes Volume: 5 Issue: 1 Pages: 666-666
  • DOI: 10.1186/1756-0500-5-666
  • Peer Reviewed
• [Journal Article] Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections (2012)
  • Author(s): Keiichi Hatakeyama, Kanako Wakabayashi-Nakao, Yutaka Aoki, Shun-ichiro Ogura, Ken Yamaguchi, Takashi Nakajima, Taka-Aki Sato, Tohru Mochizuki and Isamu Hayashi
  • Journal Title: Proteome Science Volume: 10 Issue: 1 Pages: 19-25
  • DOI: 10.1186/1477-5956-10-19
  • Peer Reviewed
• [Journal Article] Identification of a novel protein isoform derived from cancer-related splicing variants using combined analysis of transcriptome and proteome. (2011)
  • Author(s): Hatakeyama K, Ohshima K, Fukuda Y, Ogura S, Terashima M, Yamaguchi K, Mochizuki T
  • Journal Title: Proteomic Volume: 11 Issue: 11 Pages: 2275-2282
  • DOI: 10.1002/pmic.201100016
  • Peer Reviewed
• [Presentation] Survivin expression in circulating tumor cells of patients with gastric cancer (2014)
  • Author(s): Masanori Terashima, Keiichi Hatakeyama, Yushi Yamakawa, Norihiko Sugisawa, Yuichiro Miki, Rie Makuuchi, Hironobu Goto, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Keiichi Oshima and Toru Mochizuki
  • Organizer: 2014 ASCO Gastrointestinal Cancers Symposium
  • Place of Presentation: San Francisco, CA
• [Presentation] Survivin expression in circulating tumor cells of patients with gastric cancer (2014)
  • Author(s): Masanori Terashima, Keiichi Hatakeyama, Yushi Yamakawa, Norihiko Sugisawa, Yuichiro Miki, Rie Makuuchi, Hironobu Goto, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Keiichi Oshima, Toru Mochizuki
  • Organizer: 2014 ASCO Gastrointestinal Cancers Symposium
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Identification of novel protein isoforms in secretome using combined analysis of microarray and massspectrometry (2013)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Yutaka Tanizawa, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県,パシフィコ横浜
• [Presentation] Identification of the novel CEACAM5 protein isoforms ingastrointestinal cancer cells (2013)
  • Author(s): Kanako Nakao, Keiichi Hatakeyama, Keiichi Ohshima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県,パシフィコ横浜
• [Presentation] Identification of novel protein isoforms in secretome using combined analysis of microarray and mass spectrometry (2013)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Yutaka Tanizawa, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県, パシフィコ横浜
• [Presentation] Identification of the novel CEACAM5 protein isoforms in gastrointestinal cancer cells (2013)
  • Author(s): Kanako Nakao, Keiichi Hatakeyama, Keiichi Ohshima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県, パシフィコ横浜
• [Presentation] 胃癌における新規スプライシングバリアントの臨床病理学的検討 (2012)
  • Author(s): 山川雄士,谷澤豊,畠山慶一,坂東悦郎,川村泰一,徳永正則,絹笠祐介,上坂克彦,金本秀行,望月徹,寺島雅典
  • Organizer: 第84回日本胃癌学会総会
  • Place of Presentation: 大阪府,大阪国際会議場
• [Presentation] Quantification of splicing variant expression levels in peripheral bloodof patients with gastric cancer (2012)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Yutaka Tanizawa, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道,ロイトン札幌,さっぽろ芸文館,札幌市教育文化会館
• [Presentation] 癌細胞から放出されるエクソソーム中のプロテオーム解析 (2012)
  • Author(s): 大島啓一,畠山慶一,中尾香菜子,寺島雅典,山口建,望月徹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道,ロイトン札幌,さっぽろ芸文館,札幌市教育文化会館
• [Presentation] CEACAM4の甲状腺髄様癌における特異的発現 (2012)
  • Author(s): 中尾香菜子,畠山慶一,大島啓一,山口建,望月徹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道,ロイトン札幌,さっぽろ芸文館,札幌市教育文化会館
• [Presentation] 胃癌における新規スプライシングバリアントの臨床病理学的検討 (2012)
  • Author(s): 山川 雄士, 谷澤 豊, 畠山 慶一, 坂東悦郎, 川村泰一, 徳永正則 , 絹笠祐介, 上坂克彦, 金本秀行, 望月 徹, 寺島雅典
  • Organizer: 第84回日本胃癌学会総会
  • Place of Presentation: 大阪国際会議場 (大阪府)
• [Presentation] Quantification of splicing variant expression levels in peripheral blood of patients with gastric cancer (2012)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Yutaka Tanizawa, Masanori Terashima, Ken Yamaguchi, Tohru Mochizuki
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌, さっぽろ芸文館, 札幌市教育文化会館 (北海道)
• [Presentation] 癌細胞から放出されるエクソソーム中のプロテオーム解析 (2012)
  • Author(s): 大島啓一, 畠山慶一, 中尾香菜子, 寺島雅典, 山口建, 望月徹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌, さっぽろ芸文館, 札幌市教育文化会館 (北海道)
• [Presentation] CEACAM4の甲状腺髄様癌における特異的発現 (2012)
  • Author(s): 中尾香菜子, 畠山慶一, 大島啓一, 山口建, 望月徹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌, さっぽろ芸文館, 札幌市教育文化会館 (北海道)
• [Presentation] Identification of a Novel Protein Isoform Derived from Cancer-related Splicing Variants Using a Proteomics Approach (2011)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知県,名古屋国際会議場
• [Presentation] Novel Proteomic Approach for Identification of Protein Isoform Derived from Cancer-related SplicingVariants (2011)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: がん若手研究者ワークショップ文部科学省科学研究費新学術領域研究「がん研究分野の特性等を踏まえた支援活動」
  • Place of Presentation: 長野県,アートランドホテル蓼科
• [Presentation] がん関連スプライシングバリアント由来の新規プロテインアイソフォームの同定に向けた新規プロテオーム解析 (2011)
  • Author(s): 畠山慶一,大島啓一,中尾香菜子,寺島雅典,山口建,望月徹
  • Organizer: 日本プロテオーム学会2011年大会
  • Place of Presentation: 新潟県,朱鷺メッセ
• [Presentation] Identification of a Novel Protein Isoform Derived from Cancer-related Splicing Variants Using a Proteomics Approach (2011)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知県, 名古屋国際会議場
• [Presentation] Novel Proteomic Approach for Identification of Protein Isoform Derived from Cancer-related Splicing Variants (2011)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: がん若手研究者ワークショップ 文部科学省科学研究費新学術領域研究「がん研究分野の特性等を踏まえた支援活動」
  • Place of Presentation: 長野県, アートランドホテル蓼科
• [Presentation] がん関連スプライシングバリアント由来の新規プロテインアイソフォームの同定に向けた新規プロテオーム解析 (2011)
  • Author(s): 畠山慶一, 大島啓一, 中尾香菜子, 寺島雅典, 山口建, 望月徹
  • Organizer: 日本プロテオーム学会2011年大会
  • Place of Presentation: 新潟県, 朱鷺メッセ
• [Patent(Industrial Property Rights)] CEACAM5遺伝子のスプライシングバリアント (2013)
  • Inventor(s): 中尾香菜子,望月徹,畠山慶一
  • Industrial Property Rights Holder: 静岡県
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-035758
  • Filing Date: 2013-02-26
• [Patent(Industrial Property Rights)] 甲状腺髄様がん判定方法 (2013)
  • Inventor(s): 中尾香菜子,望月徹,畠山慶一
  • Industrial Property Rights Holder: 静岡県
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-12-26
• [Patent(Industrial Property Rights)] スプライスバリアント (2013)
  • Inventor(s): 望月徹,畠山慶一
  • Industrial Property Rights Holder: 静岡県
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-02-28
• [Patent(Industrial Property Rights)] 甲状腺髄様がん判定方法 (2013)
  • Inventor(s): 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Holder: 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-04-25
  • Acquisition Date: 2013-12-26
• [Patent(Industrial Property Rights)] ＣＥＡＣＡＭ５遺伝子のスプライシングバリアント (2013)
  • Inventor(s): 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Holder: 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-035758
  • Filing Date: 2013-02-26
• [Patent(Industrial Property Rights)] 甲状腺髄様がん判定方法 (2012)
  • Inventor(s): 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Holder: 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-113732
  • Filing Date: 2012-05-17
• [Patent(Industrial Property Rights)] ホルマリン固定パラフィン包埋組織切片中の蛋白質の賦活化処理システム及び賦活化処理方法 (2011)
  • Inventor(s): 青木豊,海野ゆかり,佐藤孝明,林勇,畠山慶一,小倉俊一郎
  • Industrial Property Rights Holder: 静岡県,島津製作所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2011-117334
  • Filing Date: 2011-05-25
• [Patent(Industrial Property Rights)] スプライシングバリアント (2011)
  • Inventor(s): 望月 徹, 畠山 慶一
  • Industrial Property Rights Holder: 静岡県
  • Industrial Property Number: 2011-179788
  • Filing Date: 2011