| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
We have established a DNA/PEI/chondroitin sulfate ternary complex system which mediates highly efficient tumor transfection in the living body.In this study, we found that transfection of the tumor cells with a pathogenic gene could effectively enhance the anti-tumor immunity. Those antigen expressed on the tumor cell surface would be recognized by APCs as a danger signal to provoke the immune response. We prepared the plasmid encoding pathogenic genes. Tumor-bearing mice were prepared, and transfected with those plasmids to examine the anti-tumor therapeutic effects. Transfection of the viral or bacterial antigenic protein genes showed highly effective anti-tumor activity. Co-transfection of the cytokine-genes with the pathogenic antigen-gene showed much higher anti-tumor therapeutic effect than pathogenic- orcytokine-genes alone.Transfection of the pathogenic gene was effective also on the primary tumor in dog, and apparent suppression of the tumor growth was observed.
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