PREPARATION OF SMALL PLASMID COMPLEX FOR CANCER THERAPY AND APPLICATION TO MEDIUM ANIMAL CLINICAL STUDY
| Project/Area Number |
23701103
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Clinical oncology
|
|---|
| Research Institution | Otsuma Women's University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KOYAMA Yoshiyuki 大妻女子大学, 家政学部, 教授 (00162090)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 遺伝子治療 |
|---|
| Research Abstract |
We have established a DNA/PEI/chondroitin sulfate ternary complex system which mediates highly efficient tumor transfection in the living body.In this study, we found that transfection of the tumor cells with a pathogenic gene could effectively enhance the anti-tumor immunity. Those antigen expressed on the tumor cell surface would be recognized by APCs as a danger signal to provoke the immune response. We prepared the plasmid encoding pathogenic genes. Tumor-bearing mice were prepared, and transfected with those plasmids to examine the anti-tumor therapeutic effects. Transfection of the viral or bacterial antigenic protein genes showed highly effective anti-tumor activity. Co-transfection of the cytokine-genes with the pathogenic antigen-gene showed much higher anti-tumor therapeutic effect than pathogenic- orcytokine-genes alone.Transfection of the pathogenic gene was effective also on the primary tumor in dog, and apparent suppression of the tumor growth was observed.
|
Report
(4 results)
Research Products
(28 results)
