| Project/Area Number |
23710011
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
YOSHIDA Mitsuhiro 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, ポストドクトラル研究員 (60565555)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 物質循環 / 溶存有機物 / プロテオミクス / 微生物ループ / 海洋 / シアノバクテリア / ファージ |
|---|
| Research Abstract |
In this study, the comprehensive metaproteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was performed to investigate the dissolved (< 0.2 um) proteomic composition of viral lysates occurring during infection of a marine cyanobacterial Synechococcus sp. The analysis of the lysate proteome revealed that photosynthetic proteins, metabolic enzymes involved in amino acids and carbohydrates, and viral capsids were major components, possibly indicating the formation of dissolved proteins during viral infection was influenced by differences in the nature and subcellular localization of protein molecules.
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