Elucidation of insecticide detoxification by hydrolases and evaluation of species difference between humans and experimental animals

• Project/Area Number: 23710042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: Ehime University
• Principal Investigator: NISHI Kosuke 愛媛大学, 農学部, 助教 (80578097)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 健康影響評価 / エステラーゼ / 殺虫剤 / ピレスロイド / 加水分解 / 種差

Research Abstract

A library of recombinant hydrolases expressed in human liver was generated using the baculovirus expression system. When the library was screened with pyrethroids and their derivatives containing a fluorochrome, novel enzymes that hydrolyze the insecticides were discovered. Biochemical characteristics of the enzymes was analyzed.

Research Products

• [Journal Article] Phenylhydroquinone induces loss of thymocytes through cell cycle arrest and apoptosis elevation in p53-dependent pathway (2013)
  • Author(s): Nakata Y, Nishi K, Nishimoto S, Sugahara T
  • Journal Title: J Toxicol Sci Volume: 38 Pages: 325-335
  • NAID: 10031158898
  • Peer Reviewed
• [Journal Article] Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages. (2013)
  • Author(s): Kumalasari ID, Nishi K, Harmayani E, Raharjo S, Sugahara T
  • Journal Title: J Funct Foods Volume: 5 Pages: 582-589
  • Peer Reviewed
• [Journal Article] Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells (2013)
  • Author(s): Ishida M, Nishi K, Watanabe H, Sugahara T
  • Journal Title: Food Chem Volume: 136 Pages: 322-327
  • Peer Reviewed
• [Journal Article] Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells (2013)
  • Author(s): Momoko Ishida et al.
  • Journal Title: Food Chemistry Volume: 136 Issue: 2 Pages: 322-327
  • DOI: 10.1016/j.foodchem.2012.08.079
  • Peer Reviewed
• [Journal Article] Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages (2013)
  • Author(s): Ika Dyah Kumalasari et al.
  • Journal Title: Journal of Functional Foods Volume: in press Issue: 2 Pages: 582-589
  • DOI: 10.1016/j.jff.2012.12.005
  • Peer Reviewed
• [Journal Article] Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo (2013)
  • Author(s): Ika Dyah Kumalasari et al.
  • Journal Title: Cytotechnology Volume: in press Issue: 1 Pages: 75-85
  • DOI: 10.1007/s10616-013-9539-5
  • Peer Reviewed
• [Journal Article] Phenylhydroquinone induces loss of thymocytes through cell cycle arrest and apoptosis elevation in p53-dependent pathway (2013)
  • Author(s): Yuichiro Nakata et al.
  • Journal Title: Journal of Toxicological Sciences Volume: in press
  • Peer Reviewed
• [Journal Article] Structural characterization of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016 (2012)
  • Author(s): Park S-Y, Lee H-J, Song J-M, Sun J, Hwang H-J, Nishi K, Kim J-S
  • Journal Title: Acta Crystallogr Sect Volume: D 68 Pages: 1570-1577
  • Peer Reviewed
• [Journal Article] Immunomodulatory effects of lactate dehydrogenase in vitro and in vivo. (2012)
  • Author(s): Daifuku M, Nishi K, Okamoto T, Nishimoto S, Sugahara T
  • Journal Title: J Funct Foods Volume: 4 Pages: 972-978
  • Peer Reviewed
• [Journal Article] Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo. (2012)
  • Author(s): Nishimoto S, Muranaka A, Nishi K, Kadota A, Sugahara T
  • Journal Title: J Funct Foods Volume: 4 Pages: 883-890
  • Peer Reviewed
• [Journal Article] Biologically active ester derivatives as potent inhibitors of the soluble epoxide hydrolase (2012)
  • Author(s): Kim I-H, Nishi K, Kasagami T, Morisseau C, Liu J-Y, Tsai H-J, Hammock BD
  • Journal Title: Bioorg Med Chem Lett Volume: 22 Pages: 5889-5892
  • Peer Reviewed
• [Journal Article] Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction (2012)
  • Author(s): Kanda K, Nishi K, Kadota A, Nishimoto S, Liu M-C, Sugahara T
  • Journal Title: Biochim Biophys Acta Volume: 1820 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Immunostimulatory effect of β-cryptoxanthin in vitro and in vivo (2012)
  • Author(s): Nishi K, Muranaka A, Nishimoto S, Kadota A, Sugahara T
  • Journal Title: J Funct Foods Volume: 4 Pages: 618-625
  • Peer Reviewed
• [Journal Article] Effect of collagens from jellyfish and bovine Achilles tendonon the activity of J774.1 and mouse peritoneal macrophage cells. (2012)
  • Author(s): Putra ABN, Morishige H, Nishimoto S, Nishi K, Shiraishi R, Doi M, Sugahara T
  • Journal Title: J Funct Foods Volume: 4 Pages: 504-512
  • Peer Reviewed
• [Journal Article] Immunochromatographic assay of cadmium levels in oysters (2012)
  • Author(s): Nishi K, Kim I-H, Itai T, Sugahara T, Takeyama H, Ohkawa H
  • Journal Title: Talanta Volume: 97 Pages: 262-266
• [Journal Article] Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo (2012)
  • Author(s): Kumalasari ID, Harmayani E, Lestari LA, Raharjo S, Asmara W, Nishi K, Sugahara T
  • Journal Title: Cytotechnology Volume: 64 Pages: 131-137
  • Peer Reviewed
• [Journal Article] Enzyme-linked immunosorbent assay with monoclonal and single-chain variable fragment antibodies selective to coplanar polychlorinated biphenyls (2012)
  • Author(s): Inui H, Takeuchi T, Uesugi A, Doi F, Takai M, Nishi K, Miyake S, Ohkawa H
  • Journal Title: J Agric Food Chem Volume: 60 Pages: 1605-1612
  • Peer Reviewed
• [Journal Article] (-)-Secoisolariciresinol attenuates high-fat diet-induced obesity in C57BL/6 mice (2012)
  • Author(s): Tominaga S, Nishi K, Nishimoto S, Akiyama K, Yamauchi S, Sugahara T
  • Journal Title: Food Funct Volume: 3 Pages: 76-82
  • Peer Reviewed
• [Journal Article] Immunostimulatory effects of water extract from bulbus arteriosus in tuna in vitro (2012)
  • Author(s): Daifuku M, Nishi K, Okamoto T, Nakano H, Nishimoto S, Sugahara T
  • Journal Title: J Funct Foods Volume: 4 Pages: 263-270
  • Peer Reviewed
• [Journal Article] Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction (2012)
  • Author(s): Kota Kanda et al.
  • Journal Title: Biochimica et Biophysica Acta - General Subjects Volume: 1820 Issue: 4 Pages: 461-468
  • DOI: 10.1016/j.bbagen.2011.11.015
  • Peer Reviewed
• [Journal Article] Effect of collagens from jellyfish and bovine Achilles tendon on the activity of J774.1 and mouse peritoneal macrophage cells (2012)
  • Author(s): Agus Budiawan Naro Putra et al.
  • Journal Title: Journal of Functional Foods Volume: 4 Issue: 2 Pages: 504-512
  • DOI: 10.1016/j.jff.2012.02.011
  • Peer Reviewed
• [Journal Article] Immunostimulatory effect of β-cryptoxanthin in vitro and in vivo (2012)
  • Author(s): Kosuke Nishi et al.
  • Journal Title: Journal of Functional Foods Volume: 4 Issue: 3 Pages: 618-625
  • DOI: 10.1016/j.jff.2012.04.001
  • Peer Reviewed
• [Journal Article] Biologically active ester derivatives as potent inhibitors of the soluble epoxide hydrolase (2012)
  • Author(s): In-Hae Kim et al.
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 22 Issue: 18 Pages: 5889-5892
  • DOI: 10.1016/j.bmcl.2012.07.074
  • Peer Reviewed
• [Journal Article] Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo (2012)
  • Author(s): Sogo Nishimoto et al.
  • Journal Title: Journal of Functional Foods Volume: 4 Issue: 4 Pages: 883-890
  • DOI: 10.1016/j.jff.2012.06.005
  • Peer Reviewed
• [Journal Article] Immunomodulatory effects of lactate dehydrogenase in vitro and in vivo (2012)
  • Author(s): Miho Daifuku et al.
  • Journal Title: Journal of Functional Foods Volume: 4 Issue: 4 Pages: 972-978
  • DOI: 10.1016/j.jff.2012.07.005
  • Peer Reviewed
• [Journal Article] Structural characterization of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016 (2012)
  • Author(s): Suk-Youl Park et al.
  • Journal Title: Acta Crystallographica Section D: Biological Crystallography Volume: 68 Issue: 11 Pages: 1570-1577
  • DOI: 10.1107/s0907444912038826
  • Peer Reviewed
• [Journal Article] (-)-Secoisolariciresinol attenuates high-fat diet-induced obesity in C57BL/6 mice (2012)
  • Author(s): Tominaga, S.
  • Journal Title: Food and Function Volume: 3 Issue: 1 Pages: 76-82
  • DOI: 10.1039/c1fo10166h
  • Peer Reviewed
• [Journal Article] Enzyme-linked immunosorbent assay with monoclonal and single-chain variable fragment antibodies selective to coplanar polychlorinated biphenyls (2012)
  • Author(s): Inui, H.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 60 Issue: 7 Pages: 1605-1612
  • DOI: 10.1021/jf203585b
  • Peer Reviewed
• [Journal Article] Immunomodulatory effect of (-)-matairesinol in vivo and ex vivo (2011)
  • Author(s): Yamawaki M, Nishi K, Nishimoto S, Yamauchi S, Akiyama K, Kishida T, Maruyama M, Nishiwaki H, Sugahara T
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 859-863
  • NAID: 10028272385
  • Peer Reviewed
• [Journal Article] Immunostimulation effects of yellowtail heart extracts in vitro and in vivo (2011)
  • Author(s): Daifuku M, Yaguchi Y, Nishi K, Okamoto T, Nakano H, Nishimoto S, Nishikawa S, Yamashita H, Takimoto S, Sugahara T
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 638-645
  • NAID: 10028271208
  • Peer Reviewed
• [Journal Article] Structure-activity relationships of cycloalkylamide derivatives as inhibitors of the soluble epoxide hydrolase (2011)
  • Author(s): Kim I-H, Park Y-K, Hammock BD, Nishi K
  • Journal Title: J Med Chem Volume: 54 Pages: 1752-1761
  • Peer Reviewed
• [Journal Article] Immunostimulatory in vitro and in vivo effects of a water-soluble extract from kale (2011)
  • Author(s): Nishi K, Kondo A, Okamoto T, Nakano H, Daifuku M, Nishimoto S, Ochi K, Takaoka T, Sugahara T
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 40-46
  • NAID: 10027896556
  • Peer Reviewed
• [Journal Article] Immunostimulation Effects of Yellowtail Heart Extracts in Vitro and in Vivo (2011)
  • Author(s): Daifuku, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 4 Pages: 638-645
  • DOI: 10.1271/bbb.100713
  • NAID: 10028271208
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Immunomodulatory Effect of (-)-Matairesinol in Vivo and ex Vivo (2011)
  • Author(s): M.Yamawaki, et al
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 5 Pages: 859-863
  • DOI: 10.1271/bbb.100781
  • NAID: 10028272385
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo
  • Author(s): Kumalasari ID, Nishi K, Harmayani E, Raharjo S, Sugahara T
  • Journal Title: Cytotechnology Volume: (in press)
  • Peer Reviewed
• [Presentation] Cytotoxic activity of stereoisomers of 1,7-seco-2,7'-cyclolignane (2013)
  • Author(s): Wukirasari T, Nishiwaki H, Nishi K, Sugahara T, Akiyama K, Kishida T, Yamauchi S
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
• [Presentation] Cytotoxic activity of stereoisomers of 1,7-seco-2,7'-cyclolignane (2013)
  • Author(s): Tuti Wukirasari et al.
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台市
• [Presentation] メトキシクロルのアレルギー惹起に関する研究 (2012)
  • Author(s): 安永翔, 西本壮吾, 西甲介, 菅原卓也
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] 乳酸脱水素酵素のマクロファージ活性化機構に関する研究 (2012)
  • Author(s): 大福美帆, 西甲介, 岡本威明, 菅原卓也
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] ノビレチンの脂肪細胞分化抑制効果と脂質代謝に与える影響 (2012)
  • Author(s): 大田美和, 西甲介, 門田歩, 菅原卓也
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] ホウレンソウ抽出物の脱顆粒抑制効果に関する研究 (2012)
  • Author(s): 石田萌子, 西甲介, 渡辺久, 菅原卓也
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] イムノクロマト法による牡蠣中カドミウムの測定 (2012)
  • Author(s): 西甲介, 金仁惠, 板井啓明, 菅原卓也, 竹山春子, 大川秀郎
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] Studies on the immunostimulatory effects of jellyfish collagen on macrophage (2012)
  • Author(s): Putra ABN, Morishige H, Nishimoto S, Nishi K, Shiraishi R, Doi M, Sugahara T
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: Nagoya
• [Presentation] Inhibitory effect ofaqueous spinach extract on degranulation of RBL-2H3 cells (2012)
  • Author(s): Ishida M, Nishi K, Watanabe H, Sugahara T
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: Nagoya
• [Presentation] Immunostimulatory activity of bengkoang (Pachyrhizus erosus L. Urban) fiber extracts on lymphocytes and macrophages (2012)
  • Author(s): Kumalasari ID, Nishi K, Harmayani E, Raharjo S, Sugahara T
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: Nagoya
• [Presentation] Studies on immunotoxicity of o-phenylphenol (OPP) and metabolite (2012)
  • Author(s): Sugahara T, Nakata Y, Nishi K, Nishimoto S
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: Nagoya
• [Presentation] Studies on immunotoxicity of o-phenylphenol (OPP) and metabolite (2012)
  • Author(s): Takuya Sugahara et al.
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋市
• [Presentation] Immunostimulatory activity of bengkoang (Pachyrhizus erosus L. Urban) fiber extracts on lymphocytes and macrophages (2012)
  • Author(s): Ika Dyah Kumalasari et al.
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋市
• [Presentation] Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells (2012)
  • Author(s): Momoko Ishida et al.
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋市
• [Presentation] Studies on the immunostimulatory effects of jellyfish collagen on macrophage (2012)
  • Author(s): Agus Budiawan Naro Putra et al.
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋市
• [Presentation] マグロ動脈球抽出物の免疫促進活性に関する研究 (2011)
  • Author(s): 大福美帆, 西甲介, 岡本威明, 中野博之, 西本壮吾, 菅原卓也
  • Organizer: 2011年度生物機能研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-07-02
• [Presentation] Effect of flaxseed lignan on lipid accumulation in adipocytes (2011)
  • Author(s): Sugahara T, Tominaga S, Yamauchi S, Nishimoto S, Nishi K
  • Organizer: International Conference on Food Factors (ICoFF) 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Immunoglobulin production-stimulating activity of the starch extracts from Indonesian arrowroot (Marantha arundinacea. L) (2011)
  • Author(s): Kumalasari ID, Nishi K, Harmayani E, Lestari LA, Raharjo S, Asmara W, Sugahara T
  • Organizer: International Conference on Food Factors (ICoFF) 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Immunostimulating effect of yellowtail heart extracts (2011)
  • Author(s): Daifuku M, Nishi K, Okamoto T, Nakano H, Nishimoto S, NishikawaS, Yamashita H, Takimoto S, Sugahara T
  • Organizer: International Conference on Food Factors (ICoFF) 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] ブタノール型セコシクロリグナンの合成と培養細胞に対する活性 (2011)
  • Author(s): 富山千聖, 西脇寿, 西甲介, 菅原卓也, 山内聡
  • Organizer: 2011年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎
• [Presentation] 温州ミカン果皮抽出物の免疫促進活性に関する研究 (2011)
  • Author(s): 大西紗与, 村中理子, 西甲介, 門田歩, 菅原卓也
  • Organizer: 2011年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎
• [Presentation] ハマチ心臓中に含まれる免疫促進物質に関する研究 (2011)
  • Author(s): 大福美帆, 西甲介, 菅原卓也, 岡本威明, 中野裕之, 西本壮吾, 西川智, 山下浩史, 滝本真一
  • Organizer: 2011年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎
• [Presentation] Immunoglobulin production-stimulating activity of the starch extracts from Indonesian arrowroot (Marantha arundinacea. L). (2011)
  • Author(s): Kumalasari, I. D.
  • Organizer: International Conference on Food Factors (ICoFF) 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] イムノクロマト法による牡蠣中カドミウムの測定
  • Author(s): 西甲介ら
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 愛媛県松山市
• [Presentation] ホウレンソウ抽出物の脱顆粒抑制効果に関する研究
  • Author(s): 石田萌子ら
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 愛媛県松山市
• [Presentation] ノビレチンの脂肪細胞分化抑制効果と脂質代謝に与える影響
  • Author(s): 大田美和ら
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 愛媛県松山市
• [Presentation] 乳酸脱水素酵素のマクロファージ活性化機構に関する研究
  • Author(s): 大福美帆ら
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 愛媛県松山市
• [Presentation] メトキシクロルのアレルギー惹起に関する研究
  • Author(s): 安永翔ら
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 愛媛県松山市